Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for obtaining microorganisms capable of degrading octachlorodipropyl ether from soil or sludge and octachlorodipropyl ether degrading bacteria

A technology of octachlorodipropyl ether and microbial strains, applied in the field of microorganisms, can solve the problems of long use time, endangering human health, and being difficult to decompose.

Active Publication Date: 2019-09-20
SOUTH CHINA AGRI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although octachlorodipropyl ether has been banned, because it has been used in my country for a long time, its properties are stable, it is not easy to be decomposed, it can exist in the environment for a long time, and it can endanger human health through bioaccumulation. , The effective method of degrading octachlorodipropyl ether has very important necessity and practical significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Extraction of Octachlorodipropyl Ether Degrading Bacteria

[0062] 1. A method for obtaining microorganisms capable of degrading octachlorodipropyl ether from soil or sludge, comprising the following steps:

[0063] S1. Collect soil samples or sludge from fields where pesticides have been used for a long time and mosquito coil production plants, and number them.

[0064] S2. Prepare dilution water: take a 250mL Erlenmeyer bottle and fill it with 90mL distilled water, seal it with tin foil and sterilize it with damp heat; take another 10 test tubes of 18mm×180mm each, fill it with 9mL distilled water, put rubber stoppers on it, wrap it with tin foil and tie it with a rubber band Sterilize at 121°C and 0.15MPa for 20 minutes. After sterilization, put it on the sterile operating table, turn on the ultraviolet lamp of the operating table, and cool it to room temperature for later use.

[0065] S3.LB solid medium preparation:

[0066] Recipe: tryptone 10g, yeast...

Embodiment 2

[0107] Example 2 Optimization of degradation conditions for octachlorodipropyl ether degradation solution

[0108] 1. The method of utilizing Enterobacter aerogenes strain M2016517 to degrade octachlorodipropyl ether is:

[0109] The soil containing octachlorodipropyl ether was first sterilized, and the octachlorodipropyl ether degradation solution with a concentration of 40ppm was prepared, and then the Enterobacter aerogenes strain M2016517 was inoculated in the In the degradation solution of ether, the degradation is carried out under the conditions of rotation speed of 125-175 r / min, pH of 5-9, and temperature of 20-30°C.

[0110] 2. Optimization of inoculation volume

[0111] The optimal inoculum volume was screened with different gradients of 1%, 2%, 4%, 6%, and 8% inoculum volumes as variables.

[0112] The results showed that the degradation rate was 20.7% when the inoculation amount was 1%, the highest degradation rate was 56.5% when the inoculum amount was 2%, the ...

Embodiment 3

[0126] Example 3 Octachlorodipropyl ether addition recovery extraction

[0127] 1, according to the preparation method of the octachlorodipropyl ether degradation liquid in embodiment 2, prepare 2 parts of octachlorodipropyl ether concentration and be the degradation liquid sample of 40ppm, and add quantitative octachlorodipropyl ether standard to one of them Then through the pretreatment process such as extraction, rotary evaporation, dilution, etc., take 1ml sample into the sample bottle, seal it and put it in the refrigerator to be used on the machine.

[0128] 2. The results showed that the recovery rate of standard addition was 84%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for obtaining microorganisms capable of degrading octachlorodipropyl ether from soil or sludge, and octachlorodipropyl ether-degrading bacteria with high efficiency. The method is to use the sludge or soil containing octachlorodipropyl ether collected from petrochemical plants, mosquito coil factories, farmland and other places as samples, and through the cultivation, separation, purification, domestication and screening of microbial strains, eight Chlorodipropyl ether degrading bacteria. Enterobacter aerogenes strain M2016517 with high degradability to octachlorodipropyl ether was extracted from soil and sludge for the first time in the present invention, and was preserved in Guangdong Microbial Culture Collection Center on May 17, 2016, with the preservation number GDMCC No: 60041. The degradation rate of the strain to octachlorodipropyl ether was as high as 95%. Moreover, the screening method of the present invention can achieve the purpose of strain screening and domestication at the same time, obviously shortening the domestication cycle; and it is simple, convenient and efficient, and the degrading bacteria obtained by screening have a high degradation rate to octachlorodipropyl ether.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a method for obtaining microorganisms capable of degrading octachlorodipropyl ether from soil or sludge and octachlorodipropyl ether degrading bacteria. Background technique [0002] Octachlorodipropyl ether (S421 for short), molecular formula C 6 h 6 Cl 8 O. Octachlorodipropyl ether is a pesticide synergist that can activate and synergize various pyrethroid pesticides; it is a light yellow liquid. It has a fragrance and is widely used in pesticide sprays, mosquito coils, etc. [0003] However, octachlorodipropyl ether is an organochlorine compound whose environmental behavior is similar to that of DDT. It has stable performance and a long retention time in the environment, which belongs to the class of persistent organic pollutants. Moreover, octachlorodipropyl ether will bioaccumulate in the biological chain, resulting in residues in the human body ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 吕辉雄周耀红黄雪晶陈少华杨启平邵力恒刘志健
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com