Method for acquiring microbes capable of degrading octachlorodipropyl ether from soil or sludge, and octachlorodipropyl ether-degrading bacterium

A technology of octachlorodipropyl ether and microbial strains, applied in the field of microorganisms, can solve problems such as long use time, endangering human health, and difficult to decompose

Active Publication Date: 2016-11-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although octachlorodipropyl ether has been banned, because it has been used in my country for a long time, its properties are stable, it is not easy to be decomposed, it can exist in the environment for a long time, and it can endanger human health through bioaccumulation. , The effective method of degrading octachlorodipropyl ether has very important necessity and practical significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Extraction of Octachlorodipropyl Ether Degrading Bacteria

[0062] 1. A method for obtaining microorganisms capable of degrading octachlorodipropyl ether from soil or sludge, comprising the following steps:

[0063] S1. Collect soil samples or sludge from fields where pesticides have been used for a long time and mosquito coil production plants, and number them.

[0064] S2. Prepare dilution water: take a 250mL Erlenmeyer bottle and fill it with 90mL distilled water, seal it with tin foil and sterilize it with damp heat; take another 10 test tubes of 18mm×180mm each, fill it with 9mL distilled water, put rubber stoppers on it, wrap it with tin foil and tie it with a rubber band Sterilize at 121°C and 0.15MPa for 20 minutes. After sterilization, put it on the sterile operating table, turn on the ultraviolet lamp of the operating table, and cool it to room temperature for later use.

[0065] S3.LB solid medium preparation:

[0066] Recipe: tryptone 10g, yeast...

Embodiment 2

[0107] Example 2 Optimization of degradation conditions for octachlorodipropyl ether degradation solution

[0108] 1. The method of utilizing Enterobacter aerogenes strain M2016517 to degrade octachlorodipropyl ether is:

[0109] The soil containing octachlorodipropyl ether was first sterilized, and the octachlorodipropyl ether degradation solution with a concentration of 40ppm was prepared, and then the Enterobacter aerogenes strain M2016517 was inoculated in the In the degradation solution of ether, the degradation is carried out under the conditions of rotation speed of 125-175 r / min, pH of 5-9, and temperature of 20-30°C.

[0110] 2. Optimization of inoculation volume

[0111] The optimal inoculum volume was screened with different gradients of 1%, 2%, 4%, 6%, and 8% inoculum volumes as variables.

[0112] The results showed that the degradation rate was 20.7% when the inoculation amount was 1%, the highest degradation rate was 56.5% when the inoculum amount was 2%, the ...

Embodiment 3

[0126] Example 3 Octachlorodipropyl ether addition recovery extraction

[0127] 1, according to the preparation method of the octachlorodipropyl ether degradation liquid in embodiment 2, prepare 2 parts of octachlorodipropyl ether concentration and be the degradation liquid sample of 40ppm, and add quantitative octachlorodipropyl ether standard to one of them Then through the pretreatment process such as extraction, rotary evaporation, dilution, etc., take 1ml sample into the sample bottle, seal it and put it in the refrigerator to be used on the machine.

[0128] 2. The results showed that the recovery rate of standard addition was 84%.

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Abstract

The invention discloses a method for acquiring microbes capable of degrading octachlorodipropyl ether from soil or sludge, and a high-efficiency octachlorodipropyl ether-degrading bacterium According to the method, sludge or soil containing octachlorodipropyl ether and acquired from a petrochemical factory, a mosquito-repellent incense factory, farmland or other places is used as a sample, and culture of a microbial strain, separating, purifying, domestication and screening are successively carried out so as to select out the octachlorodipropyl ether-degrading bacterium. The method provided by the invention extracts from soil and sludge an Enterobacter aerogenes strain M2016517 with high octachlorodipropyl ether degradability, and the Enterobacter aerogenes strain M2016517 is preserved in Guangdong Microbiological Culture Collection Center on May 17, 2016, with an accession number of GDMCC No. 60041. The octachlorodipropyl ether degradation rate of the strain is as high as 95%. The method provided by the invention can achieve the purposes of screening and domestication of the strain at the same time, so the domestication period is obviously shortened; moreover, the method is simple, convenient and efficient, and the screened octachlorodipropyl ether-degrading bacterium has high octachlorodipropyl ether degradation rate.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a method for obtaining microorganisms capable of degrading octachlorodipropyl ether from soil or sludge and octachlorodipropyl ether degrading bacteria. Background technique [0002] Octachlorodipropyl ether (S421 for short), molecular formula C 6 h 6 Cl 8 O. Octachlorodipropyl ether is a pesticide synergist that can activate and synergize various pyrethroid pesticides; it is a light yellow liquid. It has a fragrance and is widely used in pesticide sprays, mosquito coils, etc. [0003] However, octachlorodipropyl ether is an organochlorine compound whose environmental behavior is similar to that of DDT. It has stable performance and a long retention time in the environment, which belongs to the category of persistent organic pollutants. Moreover, octachlorodipropyl ether will bioaccumulate in the biological chain, resulting in residues in the human bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 吕辉雄周耀红黄雪晶陈少华杨启平邵力恒刘志健
Owner SOUTH CHINA AGRI UNIV
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