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Preparation method for microfluidic photoelectrochemical sensor for heart failure marker B-type natriuretic peptide

A photoelectrochemical and microfluidic technology, used in chemical instruments and methods, laboratory containers, scientific instruments, etc., can solve the problems of cumbersome detection methods, high detection limits, and high costs, achieving high repeatability, The effect of low detection limit and high detection sensitivity

Active Publication Date: 2019-11-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many detection methods for heart failure markers, such as radioimmunoassay, immunoradiometry, electrochemiluminescence analysis, etc., but most of the detection methods are cumbersome, complicated to operate, expensive, and have high detection limits. A fast, simple and sensitive detection method is of great significance

Method used

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  • Preparation method for microfluidic photoelectrochemical sensor for heart failure marker B-type natriuretic peptide
  • Preparation method for microfluidic photoelectrochemical sensor for heart failure marker B-type natriuretic peptide
  • Preparation method for microfluidic photoelectrochemical sensor for heart failure marker B-type natriuretic peptide

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Embodiment 1

[0036] A method for preparing a microfluidic photoelectrochemical sensor for detecting heart failure marker B-type natriuretic peptide, the preparation steps are as follows:

[0037] (1) Use the computer design software AUTOCAD to design and draw the channel graphics of the microfluidic chip;

[0038] (2) Use the designed graphics to draw the mask plate, and use standard soft lithography technology to process the microfluidic polydimethylsiloxane PDMS chip, including the microfluidic upper chip, the lower chip, and the gap between the upper chip and the lower chip diaphragm;

[0039] (3) The 8 cm × 3 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 min, respectively, and dried with nitrogen;

[0040] (4) Drop-coat 20 µL, 6.0 mg / mL zinc oxide ZnO solution on the conductive surface of ITO conductive glass, let it dry at room temperature, continue to drop-coat 20 µL, 3.0 mg / mL bismuth oxyiodide / bismuth sulfide BiOI / Bi 2 S 3 ...

Embodiment 2

[0046] A method for preparing a microfluidic photoelectrochemical sensor for detecting heart failure marker B-type natriuretic peptide, the preparation steps are as follows:

[0047] (1) Use the computer design software AUTOCAD to design and draw the channel graphics of the microfluidic chip;

[0048] (2) Use the designed graphics to draw the mask plate, and use standard soft lithography technology to process the microfluidic polydimethylsiloxane PDMS chip, including the microfluidic upper chip, the lower chip, and the gap between the upper chip and the lower chip diaphragm;

[0049] (3) The 8 cm × 3 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 min, respectively, and dried with nitrogen;

[0050] (4) Drop-coat 20 µL, 7.0 mg / mL zinc oxide ZnO solution on the conductive surface of ITO conductive glass, let it dry at room temperature, continue to drop-coat 20 µL, 4.0 mg / mL bismuth oxyiodide / bismuth sulfide BiOI / Bi 2 S 3 ...

Embodiment 3

[0056] A method for preparing a microfluidic photoelectrochemical sensor for detecting heart failure marker B-type natriuretic peptide, the preparation steps are as follows:

[0057] (1) Use the computer design software AUTOCAD to design and draw the channel graphics of the microfluidic chip;

[0058] (2) Use the designed graphics to draw the mask plate, and use standard soft lithography technology to process the microfluidic polydimethylsiloxane PDMS chip, including the microfluidic upper chip, the lower chip, and the gap between the upper chip and the lower chip diaphragm;

[0059] (3) The 8 cm × 3 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 min, respectively, and dried with nitrogen;

[0060] (4) Drop-coat 20 µL, 8.0 mg / mL zinc oxide ZnO solution on the conductive surface of ITO conductive glass, let it dry at room temperature, continue to drop-coat 20 µL, 5.0 mg / mL bismuth oxyiodide / bismuth sulfide BiOI / Bi 2 S 3 ...

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Abstract

The invention relates to a preparation method for a microfluidic photoelectrochemical sensor for heart failure marker B-type natriuretic peptide, which comprises four parts of a microfluidic bottom plate, a microfluidic upper chip, a microfluidic lower chip, and a diaphragm between the upper chip and the lower chip, wherein the microfluidic upper chip includes electrode slots for accommodating a counter electrode, a reference electrode, a working electrode, an injection port and microchannel and a cleaning port and microchannel; the microfluidic lower chip comprises a working electrode slot and gas valve ports and microchannels; and three photoelectrochemical electrodes are integrated on the microfluidic sensor. By using the pump control, automatic detection can be realized, and an accurate detection result can be obtained quickly without human interference. The microfluidic photoelectrochemical sensor can realize fast, efficient, sensitive, and automated detection of B-type natriuretic peptides.

Description

technical field [0001] The invention relates to a microfluidic photoelectrochemical sensor, specifically, a method for preparing the microfluidic photoelectrochemical sensor for detecting heart failure marker B-type natriuretic peptide is designed. Background technique [0002] Heart failure is the severe and terminal stage of various cardiovascular diseases, and it is an important part of the prevention and treatment of chronic cardiovascular diseases worldwide. According to statistics, the prevalence rate of heart failure in my country is 0.9%, and the prevalence rate of women is higher than that of men, and with the increase of age, the prevalence rate of heart failure increases significantly; experts said: heart failure is preventable and curable, as long as early Discovery and early treatment can greatly increase the cure rate; therefore, early diagnosis has important clinical significance for the prevention and treatment of heart failure. [0003] B-type natriuretic pe...

Claims

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Application Information

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IPC IPC(8): G01N27/327B01L3/00
CPCB01L3/502707B01L2200/027B01L2200/10G01N27/3271
Inventor 魏琴冯金慧马洪敏王雪莹马英明吴丹任祥魏贻成
Owner UNIV OF JINAN
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