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Preparation method of piezoelectric enhanced microfluidic photoelectrochemical sensor

A photoelectrochemical and microfluidic technology, applied in chemical instruments and methods, electrochemical variables of materials, scientific instruments, etc., can solve the problems of difficult to realize full automation, narrow linear range, low sensitivity, etc., to improve signal stability and Reproducibility, realization of sensitive detection, effect of accelerating electron transfer rate

Inactive Publication Date: 2021-03-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are many detection methods for sepsis markers, such as enzyme-linked immunoassay, electrochemiluminescence analysis, etc., but enzyme-linked immunoassay has methodological limitations such as low sensitivity and narrow linear range; although electrochemiluminescence analysis The detection linear range is wide and the operation is simple, but it is not easy to realize full automation

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  • Preparation method of piezoelectric enhanced microfluidic photoelectrochemical sensor

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Effect test

Embodiment 1

[0033] A method for preparing a piezoelectric-enhanced nano-array microfluidic photoelectrochemical sensor for the detection of sepsis markers, the preparation steps are as follows:

[0034] (1) Design and draw microfluidic channel graphics with computer design software AUTOCAD;

[0035] (2) Use the designed pattern to draw the mask, and process the microfluidic polydimethylsiloxane PDMS chip with standard soft lithography technology;

[0036] (3) The 5 cm × 4 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 minutes, and then dried with nitrogen, and the cleaned ITO conductive glass was etched in sequence, and the Ag / AgCI slurry The bottom plate of the micro working electrode 1 and the micro reference electrode 2 is obtained by screen printing;

[0037] (4) Drop 20 µL of 0.1 mol / L zinc nitrate and 0.1 mol / L cyclohexamethylenetetramine mixed solution on the micro-working electrode, dry it at room temperature and anneal at 300 ...

Embodiment 2

[0044] A method for preparing a piezoelectric-enhanced nano-array microfluidic photoelectrochemical sensor for the detection of sepsis markers, the preparation steps are as follows:

[0045] (1) Design and draw microfluidic channel graphics with computer design software AUTOCAD;

[0046] (2) Use the designed pattern to draw the mask, and process the microfluidic polydimethylsiloxane PDMS chip with standard soft lithography technology;

[0047] (3) The 5 cm × 4 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 minutes, and then dried with nitrogen, and the cleaned ITO conductive glass was etched in sequence, and the Ag / AgCI slurry The bottom plate of the micro working electrode 1 and the micro reference electrode 2 is obtained by screen printing;

[0048] (4) Drop 20 µL of 0.5 mol / L zinc nitrate and 0.5 mol / L cyclohexamethylenetetramine mixed solution on the micro-working electrode, dry it at room temperature and anneal at 300 ...

Embodiment 3

[0055] A method for preparing a piezoelectric-enhanced nano-array microfluidic photoelectrochemical sensor for the detection of sepsis markers, the preparation steps are as follows:

[0056] (1) Design and draw microfluidic channel graphics with computer design software AUTOCAD;

[0057] (2) Use the designed pattern to draw the mask, and process the microfluidic polydimethylsiloxane PDMS chip with standard soft lithography technology;

[0058] (3) The 5 cm × 4 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 minutes, and then dried with nitrogen, and the cleaned ITO conductive glass was etched in sequence, and the Ag / AgCI slurry The bottom plate of the micro working electrode 1 and the micro reference electrode 2 is obtained by screen printing;

[0059] (4) Drop 20 µL of 0.5 mol / L zinc nitrate and 0.5 mol / L cyclohexamethylenetetramine mixed solution on the micro-working electrode, dry it at room temperature and anneal at 300 ...

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Abstract

The invention relates to a preparation method of a piezoelectric enhanced nano array micro-fluidic photoelectrochemical sensor for procalcitonin (PCT) detection. According to the invention, a nano array ZnO / WO3 is used as a substrate material, and a piezoelectric built-in electric field assisted self-enhanced photocurrent is obtained under the pressure generated by fluid collision and the irradiation of visible light. The micro-fluidic biosensor consists of three parts, namely micro-fluidic bottom plate ITO (Indium Tin Oxide) conductive glass, a micro-fluidic chip and a silk-screen printing microelectrode, and the micro-fluidic chip comprises an electrode groove for placing a counter electrode, a reference electrode, a working electrode, a sample inlet, a micro-channel, a sample outlet anda micro-channel; iTO conductive glass is etched, Ag / AgCI slurry silk-screen printing and ZnO / WO3 nano material modification are sequentially carried out, and a bottom plate of a micro reference electrode and a bottom plate of a micro working electrode are obtained; photoelectrochemical three electrodes are integrated on the micro-fluidic biosensor, automatic detection can be realized by utilizingcontrol of a pump, and an accurate detection result can be quickly obtained without human interference. The piezoelectric enhanced micro-fluidic photoelectrochemical sensor can realize rapid, efficient, sensitive and automatic detection of a septicemia marker procalcitonin.

Description

technical field [0001] The invention relates to a piezoelectric-enhanced nano-array microfluidic photoelectrochemical sensor, in particular, a preparation method of the piezoelectric-enhanced nano-array microfluidic photoelectrochemical sensor for the detection of sepsis markers is designed. Background technique [0002] Sepsis refers to the acute systemic infection caused by pathogenic bacteria or opportunistic pathogenic bacteria invading the blood circulation, growing and multiplying in the blood, and producing toxins. Sepsis is divided into bacteremia without obvious symptoms of toxemia and sepsis with multiple abscesses. If sepsis is not quickly controlled, it can lead to tissue damage, organ dysfunction and even death. Therefore, early detection and early treatment are of great significance to the prevention and treatment of sepsis. [0003] Procalcitonin is currently one of the best markers for the diagnosis of sepsis, and it is of great significance to the early dia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/36B01L3/00
CPCG01N27/416G01N27/36B01L3/5027
Inventor 魏琴程乾冯金慧任祥马洪敏王雪莹吴丹孙旭
Owner UNIV OF JINAN
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