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Combined diagnosis paper-base micro fluidic chip, and preparation method thereof

A microfluidic chip and paper-based technology, applied in the field of biomedical detection, can solve the problems of inability to achieve quantitative detection, long detection time, and complicated operation, and achieve the effects of saving detection time, low cost, and simplifying detection steps.

Active Publication Date: 2019-11-26
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the turbidimetric and ELISA methods have high accuracy, the operation is cumbersome and the detection takes a long time
Although the colloidal gold method has short detection time and simple operation, it has low sensitivity and cannot achieve quantitative detection

Method used

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  • Combined diagnosis paper-base micro fluidic chip, and preparation method thereof
  • Combined diagnosis paper-base micro fluidic chip, and preparation method thereof
  • Combined diagnosis paper-base micro fluidic chip, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Quantum dot immunochromatography was used to detect C-reactive protein. The quantum dots were labeled on the CRP mouse monoclonal antibody and sprayed on the quantum dot reaction channel (3-3). At the same time, the CRP mouse monoclonal antibody was used as the detection line, and the goat anti-mouse IgG secondary antibody was used as the quality control line to coat the nitrocellulose membrane, and assembled with the absorbent pad and the optimized sample pad to form a test strip.

[0045] 2. Synthesis of quantum dot-labeled CRP antibody

[0046] Activation: Add the water-soluble quantum dots coated with carboxyl groups on the surface to the BS solution, add the mixed solution of NHS and BS and the solution of EDC and MES, vortex and mix, and activate by ultrasonic. Increase the volume, use a low-temperature ultracentrifuge for centrifugation, and discard the supernatant.

[0047] Coupling: Add BS solution to the activated quantum dots, reconstitute under vortex a...

Embodiment 2

[0049] Synthetic quantum dot-labeled PCT antibody

[0050] Water-soluble method of oil-soluble CdSe / ZnS quantum dots, or metal nanocluster quantum dots, or CdSe / ZnS quantum dots combined with metal nanoclusters: Precipitate with acetone and redisperse in chloroform, add an appropriate amount of mercapto Acetic acid, mix well and leave to react for 2h. Centrifuge, discard the supernatant, add buffer solution to dissolve completely, then add acetone to purify, repeat this process 2-3 times, and finally disperse the precipitate in phosphate buffer solution and store for later use.

[0051] The use of anti-anti-calcitonin monoclonal antibody and anti-calcitonin polyclonal antibody bound to two different sites of PCT, respectively, can basically rule out cross-reactivity. The monoclonal antibody is linked to the quantum dot surface by covalent cross-linking method to obtain the quantum dot-labeled monoclonal antibody. The specific process is: add quantum dots, EDC, 15 μg NHS solut...

Embodiment 3

[0053] A combined diagnostic paper-based microfluidic chip: In one embodiment, the chip is mainly composed of various functional areas of the paper base (7), and the sample feeding part (2) and the sample dividing part (6). That is, the chip (1) is disc-shaped, with a paper base (7) and a chip substrate (9) on which the paper base (7) is placed, and a sealing film (8) on the paper base (7). Three layers; set a round hole at the center of the chip (1), place the sampling part (2) in the round hole, arrange more than 2 samples evenly and symmetrically along the diameter extension line around the sampling part (2) processing area (3); each sample processing area (3) is connected to a sample dividing part (6) at the outlet along the diameter extension line; each sample dividing part is connected to a detection area (5); More than 2 screw holes (4) are set along the direction of the extension of the diameter for fixing.

[0054] In one of the embodiments, the sampling part (2) is ...

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Abstract

The invention belongs to the technical field of biomedical detection, and relates to a combined diagnosis paper-base micro fluidic chip, and a preparation method thereof. The combined diagnosis paper-base micro fluidic chip is mainly composed of a plurality of function zones of a paper base (7), a sampling part (2), and sample dividing parts (6); the combined diagnosis paper-base micro fluidic chip (1) is designed to shape like a disc, the disc is provided with three layers including the paper base (7), a chip base sheet (9) used for placing the paper base (7), and a sealing film (8) which isarranged on the paper base (7); the circular center of the chip (1) is provided with a circular hole; the sampling part (2) is arranged in the circular hole; more than 2 sample processing zones (3) are uniformly symmetrically arranged around the sampling part (2) along the diameter extending lines; a port of each sample processing zone (3) along the diameter extending line is connected with a sample dividing part (6); each sample dividing part is connected with a detection zone (5); and the chip (1) is provided with more than two screw holes (4) along the diameter extending lines for fixing.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and relates to a microfluidic chip for detecting human whole blood samples and a preparation method thereof. Background technique [0002] Immuno-chromatography (immuno-chromatography) is a rapid diagnostic technique that has risen abroad in recent years. Its principle is an immunoassay technology based on chromatography technology and antigen-antibody specific immune reaction. The stationary phase, the analyte is the mobile phase, the analyte moves on the chromatographic strip through capillary action, the analyte has a specific immune reaction at the T line, and the free substance reacts at the C line. In recent years, the rapid development of fluorescence technology has promoted the continuous breakthrough of fluorescence immunochromatography technology, and promoted the rapid development of new detection methods for the shortcomings of common methods. [0003] At present, in pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707
Inventor 徐文峰廖晓玲张念林徐鹤丹
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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