Visual detection method of Pb <2+> of polydiacetylene lipidosome in water environment

Abstract

The invention discloses a visual detection method of Pb <2+> of polydiacetylene lipidosome in a water environment. The method comprises the following steps of firstly, preparing chemical reagent raw materials required by an experiment and a dry glass instrument; then, successively carrying out synthesis of PCDA-NS, synthesis of PCDA-EDEA and synthesis of PCDA-TAA; putting a synthesized compound into a corresponding sample bottle; then, preparing the polydiacetylene lipidosome, observing forms of PDA lipidosome prepared in different proportions through a transmission electron microscope, and photographing before and after the Pb<2+> is added, and obtaining an optimal PDA lipidosome sensor according to characteristics of the forms and an appearance and color changes. By using the visual detection method of Pb <2+> of the polydiacetylene lipidosome in the water environment, large instruments and equipment are not needed, complex sample pretreatment is not needed, an analysis period is short, rapid real-time analysis can be achieved, convenience is brought to operation of workers, and practicability of a detection experiment is also improved.

Description

technical field [0001] The invention relates to the technical field of polydiacetylene liposome detection, in particular to the detection of Pb in a polydiacetylene liposome in a water environment. 2+ visual detection method. Background technique [0002] Polydiacetylene (PDA) is a conjugated polymer with special optical properties, which is widely used in the field of sensors. PDA monomers are easily dispersed in aqueous solution and can self-assemble into liposome structures. Under the irradiation of 254nm ultraviolet light, the interior of the liposome will accelerate the polymerization to form a polymer, so as to obtain a PDA polymer that changes from colorless and transparent to blue. After adding an external stimulus to the system, PDA can experience a change from blue to red. The colorimetric transition and the change from non-fluorescent to fluorescent, therefore, not only the optical changes can be easily detected using UV-vis absorption spectrum and fluorescence s...

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of polydiacetylene liposome in water environment Pb 2+ A visual detection method to solve the problem that the existing polydiacetylene liposomes in the above-mentioned background technology have low sensitivity and low recognition rate, and the presence of Pb in polydiacetylene liposomes in the water environment 2+ The visual detection of fluorophore is easily affected by the water environment, which seriously restricts the application of small molecule fluorescent probes in the water environment.