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Method for detecting miRNA-155 by using integrated paper-based dual-mode biosensor

A technology of miRNA-155 and biosensors, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of insufficient sensitivity, limited application range, large background interference, etc., to improve sensitivity, reduce background fluorescence, and reduce experimental cost effect

Inactive Publication Date: 2019-11-26
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, due to the trace amount of miRNAs in biological samples and their susceptibility to degradation, as well as the fact that these methods usually involve complex manipulations, insufficient sensitivity, expensive equipment and large background interference, their application range is very limited.

Method used

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  • Method for detecting miRNA-155 by using integrated paper-based dual-mode biosensor
  • Method for detecting miRNA-155 by using integrated paper-based dual-mode biosensor
  • Method for detecting miRNA-155 by using integrated paper-based dual-mode biosensor

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Embodiment 1

[0025] Example 1 (from human serum)

[0026] A method for constructing an integrated paper-based dual-mode device with reasonable design, low cost, easy operation and high sensitivity, which is characterized by comprising the following steps:

[0027] (1) Synthesis of CuCo-CeO by hydrothermal method 2 Nano material, and connect it with S1 chain, defined as chain 1, its base sequence is as shown in the nucleotide sequence table, as a signal probe, for H 2 o 2 Catalytic oxidation; first, 500 mg Ce (NO 3 ) 3 ·6H 2 O and 200 mg PVP were dissolved in 14 mL ethylene glycol and stirred at room temperature for 30 min; then, 0.5 mL of 20 mg / mL CuCl 2 2H 2 O and 0.5 mL of 20 mg / mL CoCl 2 ·6H 2 O solution was added to the above solution; next, the mixed solution was transferred to a Teflon-lined autoclave with a capacity of 20 mL, and heated at 160 °C for 8 h; after the reaction was completed, the autoclave was naturally cooled to At room temperature, the product was collected a...

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Abstract

The invention provides a method for detecting miRNA-155 by using an integrated paper-based dual-mode biosensor and belongs to the technical field of miRNA detection. According to the method of the invention, the integrated paper-based dual-mode biosensor is constructed; according to the integrated paper-based dual-mode biosensor, an S1@CuCo-CeO2 nano material is adopted as a signal amplifier and is used for the ultra-sensitive sensing of miRNA-155. In order to achieve the objective, firstly, CuCo-CeO2 nanospheres are synthesized to serve as signal probes, and then gold nanoparticles grow on the surface of a paper-based working electrode in situ so as to improve conductivity and promote the modification of miRNA-155 modified hairpin probes; through a hybridization chain reaction, the CuCo-CeO2 can be successfully fixed on the surface of the paper-based working electrode and is used for quantitatively detecting the miRNA-155; and dual-mode signal reading may be achieved by simply converting the spatial configuration of the paper-based biosensor.

Description

technical field [0001] The invention relates to a method for detecting miRNA-155 by an integrated paper-based dual-mode biosensor, belonging to the technical field of miRNA detection. Background technique [0002] MicroRNAs (miRNAs), endogenous and single-stranded RNAs of approximately 22 nucleotides in length, are critical for post-transcriptional regulation of gene expression and are associated with the development of various cancers. MicroRNA-155, as one of the typical non-protein-coding miRNAs involved in various biological processes, has become a potential biomarker for early screening and diagnosis of cancers such as liver cancer, leukemia and cardiovascular diseases. Therefore, it is of great significance to explore strategies for rapid, precise and ultrasensitive detection of miRNA-155. [0003] To achieve ultrasensitive detection of miRNA-155, several analytical techniques for detecting miRNA-155 have been developed, including plasmon resonance, electrochemilumines...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/26
CPCG01N27/26G01N27/3278
Inventor 于京华周晨曦崔康李丽刘悦刘婷婷
Owner UNIV OF JINAN
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