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Preparation method of OCMCS-modified iron oxide nanoparticles coupling folic acid and transferrin

A technology of iron oxide nanometer and transferrin, which is applied in the field of medical materials, can solve the problems of unstable surface chemical properties, easy aggregation of nanoparticles, lack of active functional groups, etc., to increase blood circulation time, high reaction temperature, and improve water solubility Effect

Pending Publication Date: 2019-11-29
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the easy aggregation of nanoparticles, unstable surface chemical properties, easy oxidation and lack of active functional groups on the surface, it is difficult to apply directly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) Weigh 15.0g of PEG into a 50mL three-neck flask, heat to 80°C under constant stirring and mixing, and add 0.7g of Fe(acac) into the flask 3 , keep warm for 10 minutes, pass argon gas in the three-necked flask to prevent the reactants from being oxidized, and the flow rate is 200mL / min;

[0042] 2) Heat up to 260°C at a heating rate of 7.2°C / min, stop heating after 1 hour of reaction, and take out the sample after air cooling to 60°C;

[0043] 3) Transfer the cooled sample into a beaker, add 60mL of toluene and perform ultrasonic dispersion, then magnetically separate to obtain a black precipitate, discard the supernatant, and wash twice;

[0044] 4) The obtained black precipitate was washed twice with acetone again, and the sample was vacuum-dried at 60° C. to obtain a black powder;

[0045] 5) Weigh 10 mg of iron oxide and 50 mg of carboxymethyl chitosan (OCMCS), dissolve them in chloroform by ultrasonication, add 10 mL of deionized water dropwise while ultrasonica...

Embodiment 2

[0055] 1) Weigh 17.5g of PEG into a 50mL three-necked flask, heat to 80°C under constant stirring and mixing, and add 1.05g of Fe(acac) into the flask 3 , keep warm for 15 minutes, pass argon gas in the three-necked flask to prevent the reactants from being oxidized, and the flow rate is 200mL / min;

[0056] 2) Heat up to 260°C at a heating rate of 7.2°C / min, stop heating after 1 hour of reaction, and take out the sample after air cooling to 60°C;

[0057] 3) Transfer the cooled sample into a beaker, add 90mL of toluene and perform ultrasonic dispersion, then magnetically separate to obtain a black precipitate, discard the supernatant, and wash repeatedly 2 times;

[0058] 4) The obtained black precipitate was washed twice with acetone again, and the sample was vacuum-dried at 60° C. to obtain a black powder;

[0059] 5) Weigh 12.5 mg of iron oxide and 65 mg of carboxymethyl chitosan (OCMCS), dissolve them in chloroform by ultrasonication, add 15 mL of deionized water dropwise...

Embodiment 3

[0068] 1) Weigh 20.0g of PEG into a 50mL three-neck flask, heat to 80°C under constant stirring and mixing, and add 1.4g of Fe(acac) into the flask 3 , keep warm for 20min, pass argon gas in the three-necked flask so that the reactants will not be oxidized, and the flow rate is 200mL / min;

[0069] 2) Heat up to 260°C at a heating rate of 7.2°C / min, stop heating after 1 hour of reaction, and take out the sample after air cooling to 60°C;

[0070] 3) Transfer the cooled sample into a beaker, add 120mL of toluene and perform ultrasonic dispersion, then magnetically separate to obtain a black precipitate, discard the supernatant, and wash repeatedly 2 times;

[0071] 4) The obtained black precipitate was washed twice with acetone again, and the sample was vacuum-dried at 60° C. to obtain a black powder;

[0072] 5) Weigh 15 mg of iron oxide and 80 mg of carboxymethyl chitosan (OCMCS), dissolve them in chloroform by ultrasonication, add 20 mL of deionized water dropwise while ultr...

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Abstract

The invention relates to the field of medical materials, and discloses a preparation method of OCMCS-modified iron oxide nanoparticles coupling folic acid and transferrin. The method comprises the following steps of: firstly, preparing superparamagnetic nano iron oxide particles by using iron acetylacetonate as an iron source through a high-temperature thermal decomposition method, wherein carboxymethyl chitosan has amino groups and hydroxyl groups and further modifies the surfaces of the iron oxide particles so as to obtain Fe3O4@OCMCS; then adding transferrin, and performing ultrasonic shaking table incubation to obtain Fe3O4@OCMCS-Tf; finally, adding activated folic acid, and performing reaction in the dark under strong stirring so as to obtain Fe3O4@OCMCS-Tf-FA which has multiple targeting properties.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a method for preparing iron oxide nanoparticles modified with OCMCS and coupled with folic acid and transferrin. Background technique [0002] Cancer (malignant tumor) is one of the refractory diseases that seriously endanger human health, with high morbidity and mortality every year. Therefore, the study of tumor formation and treatment methods has become the research focus and hotspot of researchers. In recent years, the combination of biomedicine and nanotechnology has brought new opportunities for cancer treatment. Nanomedicines with unique optical, magnetic, electrical, and acoustic properties have brought new ideas to the prevention, diagnosis, and treatment of major diseases. [0003] Superparamagnetic iron oxide nanoparticles are widely used in targeted drug delivery, sensors, rapid bioseparation, magnetic resonance imaging, magnetic hyperthermia and other fields due to...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/61A61K47/62A61K47/54A61K41/00A61K45/00A61P35/00
CPCA61K47/6929A61K47/61A61K47/62A61K47/545A61K41/0052A61K45/00A61P35/00
Inventor 王秉徐城锋蒋鹏王翠彭志勤胡智文
Owner ZHEJIANG SCI-TECH UNIV
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