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Method for tissue culture and rapid propagation of traditional Chinese medicine Daphne odora Thunb.var.atrocaulis Rehd.

A technology for tissue culture and fast propagation of Rhizoma rhizoma, applied in the field of plant tissue culture, can solve the problems of no patent for tissue culture and rapid propagation of Rhizoma rhododendron, and few researches on the breeding technology of Rhizoma rhizoma Good quality seedlings

Inactive Publication Date: 2019-12-03
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Judging from the published literature, the research on Daphne maudensis mainly focuses on the chemical components, identification of crude drugs and formulation of quality standards, etc. There is very little research on the breeding technology of Daphne mauraceae, and there is no application for a patent for tissue culture and rapid propagation of Daphne maudensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Collection of explants: In the wild in Fangchenggang City, Guangxi, select the middle and upper parts of the plants with strong growth and no obvious diseases, and the sectioned stems with sufficient light as explants. .

[0020] (2) Explant induction: the explants collected in step (1) and returned to the laboratory were rinsed with tap water for 8 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 30 seconds, rinsed with sterile water After 7 times, dry the water with sterile filter paper, sterilize it in 0.5% sodium hypochlorite solution for 25 minutes, rinse it with sterile water for 10 times, dry it with sterile filter paper, and cut it into 2.0-2.5cm sectioned stems segment and inoculated into the induction medium, cultured at 25°C in total darkness for 50 days to induce the formation of adventitious buds, and the induction rate reached 94%. Described induction medium is: B 5 Medium+2.5mg / L KT+1mg / L GA+30g / L sucrose+4g / L aga...

Embodiment 2

[0026] (1) Collection of explants: In the field of Yulin City, Guangxi, the middle and upper parts of the plants with strong growth and no obvious diseases, and the sectioned stems with sufficient light were selected as explants. After collection, they were treated with water retention and moisturizing and brought back to the laboratory in time. .

[0027] (2) Explant induction: The explants collected in step (1) and returned to the laboratory were rinsed under tap water for 4 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 25 seconds, sterile water Rinse 5 times, blot dry with sterile filter paper, sterilize in 0.5% sodium hypochlorite solution for 20 minutes, rinse 7 times with sterile water, blot dry with sterile filter paper, cut into 2.0-2.5cm strips The stem segments are inoculated into the induction medium, placed at 25° C. and cultured in total darkness for 55 days to induce the formation of adventitious buds, and the induction rate...

Embodiment 3

[0033] (1) Collection of explants: In the field of Wuzhou City, Guangxi, select the middle and upper part of the plant of Daphne chrysantha with no obvious diseases, and the sectioned stems with sufficient light as explants. After collection, they should be treated with water retention and moisturizing and brought back to the laboratory .

[0034](2) Explant induction: The explants collected in step (1) and returned to the laboratory were rinsed under tap water for 4 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 20 seconds, sterile water Rinse 5 times, blot dry with sterile filter paper, sterilize in 0.5% sodium hypochlorite solution for 18 minutes, rinse 5 times with sterile water, blot dry with sterile filter paper, cut into 2.0-2.5cm strips The stem segments are inoculated into the induction medium, and cultured at 25° C. in total darkness for 60 days to induce the formation of adventitious buds, and the induction rate reaches 90%. De...

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PUM

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Abstract

The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of traditional Chinese medicine Daphne odora Thunb.var.atrocaulis Rehd.. The method sequentially includes the following steps of Daphne odora Thunb.var.atrocaulis Rehd. explant obtaining, explant induction culture, proliferation culture, rooting culture and transplanting; an explant induction culture medium for explant induction culture operation comprises a B5 medium, 1.5-2.5 mg / L of KT, 0.5-1.0 mg / L of GA, 20-30 g / L of sucrose, 3.5-4.0 g / L of agarophyte, 100-500 g / L of PVP and 10-20 ml / L of coconut milk, and the pH value is 5.6-5.9. According to the method, a Daphne odora Thunb.var.atrocaulis Rehd. section with canes is used as an explant, after the steps of induction culture, proliferation culture, rooting culture and transplanting, the inductivity reaches 90% or above, tissue culture and rapid propagation of Daphne odora Thunb.var.atrocaulis Rehd. are achieved, and thus the purpose of the method is achieved.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of the traditional Chinese medicine Rhizoma mauritius. Background technique [0002] Daphne odora Thunb.var.atrocaulis Rehd., also known as Daphne odora Thunb.var.atrocaulis Rehd., is a Daphne odora plant of Daphneaceae. It is a unique medicinal plant in my country. It is distributed in Jiangsu, Anhui, Zhejiang, Jiangxi, Taiwan, In Hubei, Hunan, Guangdong, Guangxi, Sichuan, Guizhou and other places, the stem bark and roots are used as medicine, which can be used for medicine. It has the effects of promoting blood circulation, reducing swelling and relieving sore throat. [0003] Judging from the published literature, the research on Daphne maudensis mainly focuses on the chemical components, identification of crude drugs and formulation of quality standards, etc. There is very little research on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 莫昭展
Owner YULIN NORMAL UNIVERSITY
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