Functional nanometer material-antigen composite nanoparticles and preparation method and application thereof

A technology of composite nanoparticles and nanomaterials, which is applied in the field of functionalized nanomaterial-antigen composite nanoparticles and its preparation, and material preparation, which can solve the problems that the ideal tumor immune effect cannot be achieved.

Inactive Publication Date: 2019-12-03
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] According to one aspect of the present application, a functionalized nanomaterial-antigen composite nanoparticle and its preparation method are provided, which avoids the shortcoming that the ideal tumor immune effect cannot be achieved caused by the weak immunogenicity of the free antigen and solves the problem of current imaging and Separate issues of tumor immunotherapy, achieving the dual purpose of fluorescence imaging or magnetic resonance imaging and tumor immunotherapy

Method used

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  • Functional nanometer material-antigen composite nanoparticles and preparation method and application thereof
  • Functional nanometer material-antigen composite nanoparticles and preparation method and application thereof
  • Functional nanometer material-antigen composite nanoparticles and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0088] First prepare fluorescent carbon quantum dots, take 1 g of citric acid, 1 g of PEG-1500, and 10 mL of triethanolamine, mix and stir, add 5 mL of ethylenediamine when heated to 160 ° C, stir thoroughly for 60 min, and wait for the mixture to cool to room temperature , add 50mL of deionized water, and transfer to a 2000Da dialysis bag, dialyze for 3 days, and freeze-dry to obtain carbon quantum dot powder with a size of 1-5nm.

[0089] Take 5 mg of the prepared carbon quantum dot powder and dissolve it in deionized water, add 10 mg / mL EDC 0.5 mL, stir at room temperature for 30 min, centrifuge at 3500 g for 60 min in a 3000Da ultrafiltration tube to remove excess EDC; add 2 mL of deionized water to resuspend carbon quantum dots, add 10 mg / mL NHS 0.5mL and 8mg / mL ovalbumin 1mL, react at room temperature for 16h, and finally obtain carbon quantum dots-antigen ovalbumin composite nanoparticles aqueous solution. The obtained carbon quantum dots-antigen ovalbumin composite na...

Embodiment 2

[0092] First prepare fluorescent carbon quantum dots, take 1 g of citric acid, 1 g of PEG-1500, and 10 mL of triethanolamine, mix and stir, add 5 mL of ethylenediamine when heated to 160 ° C, stir thoroughly for 60 min, and wait for the mixture to cool to room temperature , add 50mL of deionized water, and transfer to a 2000Da dialysis bag, dialyze for 3 days, and freeze-dry to obtain carbon quantum dot powder with a size of 1-5nm.

[0093] Take 5 mg of the prepared carbon quantum dot powder and dissolve it in deionized water, add 0.5 mL of 10 mg / mL EDC and stir at room temperature for 30 min, then centrifuge at 3500 g for 30 min in a 3000 Da ultrafiltration tube to remove excess EDC; add 2 mL of deionized water to resuspend the carbon quantum dots, add 10 mg / mL NHS 0.5mL and 0.8mg / mL ovalbumin polypeptide 1mL, react at room temperature for 18h, and finally obtain carbon quantum dots-antigen ovalbumin polypeptide composite nanoparticles aqueous solution. The obtained carbon q...

Embodiment 3

[0095]First prepare iron ferric oxide nanoparticles, 12.6mmol FeCl 3 ·6H 2 O was dissolved in 12 mL of deionized water, followed by the addition of 34.5 mmol of sodium oleate. 45mL of n-hexane and 25mL of ethanol mixture were added to the reaction mixture and added to 70°C for 4 hours. After the reaction, the top organic layer (ie ferric oleate) was obtained through a separatory funnel, and washed three times with deionized water. The n-hexane was removed by low-speed rotary evaporation to obtain iron oleate nanoparticles.

[0096] Fe3O4 nanoparticles were obtained by ferric oleate, oleyl alcohol and octadecenoic acid. Dissolve 2g of iron oleate complex in 20mL of oleyl alcohol and 0.3mL of octadecenoic acid mixture. After nitrogen degassing, reflux at 320° C. for 60 minutes under nitrogen protection. The solution turned from brown to black. After cooling to room temperature, 50 mL of acetone was added to stabilize the nanoparticles, and the nanoparticles were separated ...

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Abstract

The application discloses functional nanometer material-antigen composite nanoparticles and a preparation method and application thereof. The composite nanoparticles comprise a functional nanometer material and an antigen. The preparation method is simple, the defect that a free antigen is weak in immunogenicity to cause that ideal tumor immune efficacy cannot be achieved, is overcome, and the problem that at current, imaging and tumor immunotherapy are separate, can be solved. The dual purposes of fluorescent imaging or magnetic resonance imaging and tumor immunotherapy can be achieved.

Description

technical field [0001] The application relates to a functionalized nanomaterial-antigen composite nanoparticle and its preparation method and application, belonging to the field of material preparation method and application. Background technique [0002] Malignant tumor is the most threatening disease to human health and life after cardiovascular and cerebrovascular diseases, and its incidence is still increasing year by year. According to the forecast of the World Health Organization, there will be 20 million new cases of tumors in 2020, including 12 million deaths. Tumors will become the number one killer of human beings in the new century. [0003] Cancer treatment is still a difficult problem in the medical field. As the three conventional means of surgery, radiotherapy and chemotherapy cannot cure all tumors, and the specificity of the above three conventional treatment methods is insufficient, often causing varying degrees of damage to normal tissues and organs. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/18A61K39/00A61K39/39A61P35/00
CPCA61K39/00A61K39/39A61K49/0067A61K49/1824A61K47/52A61P35/00
Inventor 吴爱国罗利嘉祖柏儿
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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