Functional nanometer material-antigen composite nanoparticles and preparation method and application thereof
A technology of composite nanoparticles and nanomaterials, which is applied in the field of functionalized nanomaterial-antigen composite nanoparticles and its preparation, and material preparation, which can solve the problems that the ideal tumor immune effect cannot be achieved.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0088] First prepare fluorescent carbon quantum dots, take 1 g of citric acid, 1 g of PEG-1500, and 10 mL of triethanolamine, mix and stir, add 5 mL of ethylenediamine when heated to 160 ° C, stir thoroughly for 60 min, and wait for the mixture to cool to room temperature , add 50mL of deionized water, and transfer to a 2000Da dialysis bag, dialyze for 3 days, and freeze-dry to obtain carbon quantum dot powder with a size of 1-5nm.
[0089] Take 5 mg of the prepared carbon quantum dot powder and dissolve it in deionized water, add 10 mg / mL EDC 0.5 mL, stir at room temperature for 30 min, centrifuge at 3500 g for 60 min in a 3000Da ultrafiltration tube to remove excess EDC; add 2 mL of deionized water to resuspend carbon quantum dots, add 10 mg / mL NHS 0.5mL and 8mg / mL ovalbumin 1mL, react at room temperature for 16h, and finally obtain carbon quantum dots-antigen ovalbumin composite nanoparticles aqueous solution. The obtained carbon quantum dots-antigen ovalbumin composite na...
Embodiment 2
[0092] First prepare fluorescent carbon quantum dots, take 1 g of citric acid, 1 g of PEG-1500, and 10 mL of triethanolamine, mix and stir, add 5 mL of ethylenediamine when heated to 160 ° C, stir thoroughly for 60 min, and wait for the mixture to cool to room temperature , add 50mL of deionized water, and transfer to a 2000Da dialysis bag, dialyze for 3 days, and freeze-dry to obtain carbon quantum dot powder with a size of 1-5nm.
[0093] Take 5 mg of the prepared carbon quantum dot powder and dissolve it in deionized water, add 0.5 mL of 10 mg / mL EDC and stir at room temperature for 30 min, then centrifuge at 3500 g for 30 min in a 3000 Da ultrafiltration tube to remove excess EDC; add 2 mL of deionized water to resuspend the carbon quantum dots, add 10 mg / mL NHS 0.5mL and 0.8mg / mL ovalbumin polypeptide 1mL, react at room temperature for 18h, and finally obtain carbon quantum dots-antigen ovalbumin polypeptide composite nanoparticles aqueous solution. The obtained carbon q...
Embodiment 3
[0095]First prepare iron ferric oxide nanoparticles, 12.6mmol FeCl 3 ·6H 2 O was dissolved in 12 mL of deionized water, followed by the addition of 34.5 mmol of sodium oleate. 45mL of n-hexane and 25mL of ethanol mixture were added to the reaction mixture and added to 70°C for 4 hours. After the reaction, the top organic layer (ie ferric oleate) was obtained through a separatory funnel, and washed three times with deionized water. The n-hexane was removed by low-speed rotary evaporation to obtain iron oleate nanoparticles.
[0096] Fe3O4 nanoparticles were obtained by ferric oleate, oleyl alcohol and octadecenoic acid. Dissolve 2g of iron oleate complex in 20mL of oleyl alcohol and 0.3mL of octadecenoic acid mixture. After nitrogen degassing, reflux at 320° C. for 60 minutes under nitrogen protection. The solution turned from brown to black. After cooling to room temperature, 50 mL of acetone was added to stabilize the nanoparticles, and the nanoparticles were separated ...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com