Low Nitrogen Dependent High Efficiency Calcium Mineralizing Bacillus and Its Application in Concrete Repair
A Bacillus-dependent technology for bacteria, building repairs, microbe-based methods, etc., to address issues such as diminished durability, micro-cracks, and concrete integrity damage
Active Publication Date: 2022-06-21
SHENZHEN UNIV
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Problems solved by technology
However, with the increase of the service life and various environmental conditions, such as the influence of physical or chemical erosion such as atmosphere and water, micro-cracks will begin to appear on the surface and inside of the concrete
If effective treatment measures are not taken in time, once the cracks expand, the internal steel structure will be corroded, resulting in damage to the integrity of the concrete, and the durability will be weakened (environmental erosion of reinforced concrete)
Method used
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Embodiment Construction
[0012] The acquisition process and the characteristics of the strains involved in the present invention will be described in detail below with reference to the accompanying drawings.
[0013] Isolation, Purification and Preservation of Bacillus B8
[0014] The calcium ion mineralizing bacteria are obtained through enrichment, screening and purification, and the specific steps are as follows:
[0015] Enrichment culture: 1 g each of saline-alkali lake sediment and mangrove sediment soil samples were added to nitrogen-free LN-seawater-sodium carbonate culture solution, and cultured with shaking on a shaker at 30°C and 150 rpm for 7 days.
[0016] Separation and culture: Pipette 200 μL of the enriched culture solution and add it to the surface of the nitrogen-free LN-seawater-sodium carbonate solid medium plate, spread it evenly with a sterile coating rod, and then continue to use the coating rod to spread 3 nitrogen-free LN- Seawater-sodium carbonate solid medium plates were in...
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Abstract
The present invention is a low-nitrogen-dependent high-efficiency calcium-mineralizing bacillus and its application in concrete repair. The high-efficiency calcium-mineralizing bacillus strain has a Latin name of Bacillus sp.B8, which was identified as Bacillus and was released in 2019 On July 17, it was preserved in the General Microorganism Center of China Culture Collection Management Committee, and the preservation number is: CGMCC NO.18239; the bacteria still has high-efficiency calcium ion deposition activity under the condition of nitrogen source concentration as low as 0.5g / L, and the initial cell The concentration is 1×10 8 pc / ml, after 30 days of cultivation, the output of calcium carbonate on the glass test tube wall is as high as 38mmol / L. It has good reproduction ability and the ability to induce calcium carbonate deposition in a strong alkaline environment, and can be used for the repair of concrete cracks .
Description
[Technical field] [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-efficiency calcium-mineralizing alkalophilic bacillus and its application in concrete repair. [Background technique] [0002] As one of the most widely used civil engineering materials in the world, concrete is widely used in modern construction projects because of its firm structure, strong durability, abundant raw materials and low price. However, with the increase of service life and various environmental conditions, such as the influence of physical or chemical corrosion such as atmosphere and water, micro-cracks will begin to appear on the surface and inside of concrete. If effective treatment measures are not taken in time, once the cracks expand, the internal reinforced structure will be corroded, resulting in the destruction of concrete integrity and the weakening of its durability (environmental erosion of reinforced concrete). However, some scholars ha...
Claims
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IPC IPC(8): C12N1/20C12P3/00E04G23/02C12R1/07
CPCC12P3/00E04G23/0203C12R2001/07C12N1/205
Inventor 张金龙邓旭邢锋刘冰韩宁旭黄兴桃冯咏谢乐梁智勇罗顺
Owner SHENZHEN UNIV