Cosmetic material composition containing epipinephrine
A technology of cosmetic materials and compositions, applied in the direction of active ingredients of heterocyclic compounds, cosmetics, drug combinations, etc., can solve skin irritation and other problems, achieve the effects of preventing skin aging, promoting collagen biosynthesis, and promoting biosynthesis
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Embodiment 1
[0060] Example 1. Preparation of samples
[0061] Epirosinol used in this example was purchased from Coombo Research Chemicals, which was dissolved in DMSO as a solvent at concentrations of 1 μg / ml and 10 μg / ml, and used in the following experiments.
Embodiment 2
[0062] Example 2. Confirmation of the collagenase inhibitory effect of epipinephrine
[0063] The samples were dissolved in DMSO as a solvent at concentrations of 1 μg / ml and 10 μg / ml and used in the following experiments.
[0064] Specifically, a human fibroblast cell line (Human dermal fibroblast, Hs68, ATCC) was tested under the following conditions. First, Hs68 fibroblasts were divided into 2 × 104 Dispense into 96-well plates, then incubate at 37°C, 5% CO 2 conditioned incubator for 24 hours. Then, remove the medium, put it in DPBS, use 15mJ / cm 2 The remaining cell groups except the UVB non-irradiated group were UVB irradiated, and then epipinosinol was added at different concentrations and further cultured for 24 hours.
[0065] After RNA was isolated from the cells of each sample using tricyclazole (RNA iso, DAKARA, Japan), RNA was quantified at 260 nm with a nanodrop, and cDNA was synthesized in an amplifier using 2 μg of RNA (C1000 ThermalCycler, Bio- Rad, USA)....
Embodiment 3
[0070] Example 3. Confirmation of the IL-6 inhibitory effect of epipinephrine
[0071] In order to confirm the IL-6 inhibitory effect of epipinephrine, experiments were performed under the same conditions using the same cells as described in Example 1. Only the IL-6 primer was used instead of the MMP-1 primer during the final real-time polymerase chain reaction. Finally, the expression level of the gene is analyzed by correcting the β-actin gene, and the analysis results are as follows: figure 2 shown.
[0072] like figure 1 and figure 2 As shown, the IL-6 gene expression level was significantly decreased in a concentration-dependent manner in the epipinosinol-treated group in the same manner as the MMP-1 gene expression level.
[0073] From these results, it can be seen that epirosinol exhibits a wrinkle-improving effect and a skin irritation-relieving effect by reducing the expression of the inflammatory cytokine IL-6.
[0074] Table 2
[0075]
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