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Technology for chromatographic extraction of glutamic acid after centrifugal thallus removal

A technology of centrifugal sterilization and glutamic acid, which is applied in the preparation of organic compounds, chemical instruments and methods, organic chemistry, etc., can solve the problems of large amount of acid and alkali, large amount of sewage, etc., achieve less waste water output and promote water saving , the effect of good industrial practical value

Inactive Publication Date: 2019-12-10
卢松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this process has defects such as large amount of sewage and large amount of acid and alkali.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A process for extracting glutamic acid by chromatography after centrifugation, comprising the steps of:

[0030] Filter 1000L of glutamic acid fermentation liquid (13.6g / 100ml) with a ceramic membrane with a membrane pore size of 0.08μm to remove bacterial protein, collect the filtrate, and then filter the filtrate with an average 5m 3 The flow rate of / h is injected into the sequential simulated moving bed chromatographic separation, and the temperature of the feed liquid is kept at 70°C, the extract after the chromatographic separation is collected, and then evaporated and concentrated by a plate evaporator, the concentration temperature is 70°C, and the vacuum degree is 0.05MPa , concentrated 2 times, and then enter the isoelectric crystallization tank for crystallization, adjust the pH to 3.2, collect 121.4kg of wet crystals and 390L of isoelectric supernatant (1.2g / 100ml);

[0031] The separation conditions and parameters of the simulated moving bed chromatography ...

Embodiment 2

[0035] A process for extracting glutamic acid by chromatography after centrifugation, comprising the steps of:

[0036] Filter 1000L of glutamic acid fermentation broth (14.7g / 100ml) with a ceramic membrane with a membrane pore size of 0.1μm to remove bacterial protein, collect the filtrate, and then filter the filtrate with an average 5m 3 The flow rate of / h is injected into the sequential simulated moving bed chromatographic separation, and the temperature of the feed liquid is kept at 70°C, the extract after the chromatographic separation is collected, and then evaporated and concentrated by a plate evaporator, the concentration temperature is 70°C, and the vacuum degree is 0.05MPa , concentrated 2 times, and then enter the isoelectric crystallization tank for crystallization, adjust the pH to 3.2, collect 127.3kg of wet crystals and 380L of isoelectric supernatant (1.3g / 100ml);

[0037] The separation conditions and parameters of the simulated moving bed chromatography ar...

Embodiment 3

[0041] A process for extracting glutamic acid by chromatography after centrifugation, comprising the steps of:

[0042] Filter 1000L (12.8g / 100ml) of glutamic acid fermentation broth with a ceramic membrane with a membrane pore size of 0.05μm to remove bacterial protein, collect the filtrate, and then filter the filtrate with an average of 4m 3 The flow rate of / h is injected into the sequential simulated moving bed chromatographic separation, and the temperature of the feed liquid is kept at 70°C, the extract after the chromatographic separation is collected, and then evaporated and concentrated by a plate evaporator, the concentration temperature is 70°C, and the vacuum degree is 0.05MPa , concentrated 2 times, and then enter the isoelectric crystallization tank for crystallization, adjust the pH to 3.2, collect 119.6kg of crystals and 395L of isoelectric supernatant (1.1g / 100ml);

[0043] The separation conditions and parameters of the simulated moving bed chromatography ar...

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Abstract

The invention belongs to the technical field of amino acid separation and purification, and discloses a process for chromatographic extraction of glutamic acid after centrifugal thallus removal, whichcomprises the following steps: step 1) filtration and sterilization, step 2) chromatographic separation, step 3) resin column adsorption, and step 4) concentration and crystallization. The method hasthe advantages of low wastewater yield, low environmental pressure and higher glutamic acid yield, and has favorable industrial practical value.

Description

technical field [0001] The invention belongs to the technical field of amino acid separation and purification, and in particular relates to a process for extracting glutamic acid by chromatography after centrifugation and bacteria removal. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid was discovered by Li Suxun in 1856. It is a colorless crystal with umami taste, slightly soluble in water, but soluble in hydrochloric acid solution, with an isoelectric point of 3.22. A large amount exists in cereal protein, and the content in animal brain is also more. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. MSG is prepared from glutamic acid. [0003] The existing technology of extracting glutamic acid from glutamic acid ferme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/40C07C229/24
CPCC07C227/40
Inventor 卢松田永红梁晓娟王文强高雷王斌郄子玥高启超
Owner 卢松
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