Polypeptide, polypeptide derivative, and polypeptide-antibody complex and preparation and application of polypeptide

A technology of antibody complexes and peptide derivatives, applied in the direction of antibodies, drug combinations, antibody medical components, etc., can solve the problems of low targeting, cumbersome production process, high cost, etc., and achieve enhanced targeting and simple preparation process. , the effect of low cost

Inactive Publication Date: 2019-12-10
安徽瑞达健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Aiming at the problems of cumbersome production process, high cost and low targeting existing in existing methods for enhancing the targeting of T cells, the present invention provides a polypeptide, which can significantly enhance the targeting of immune cells, and at the same time The preparation process of the polypeptide is simple, and the preparation cost is lower than the existing cost of preparing CAR-T cells and bispecific antibody cells

Method used

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  • Polypeptide, polypeptide derivative, and polypeptide-antibody complex and preparation and application of polypeptide
  • Polypeptide, polypeptide derivative, and polypeptide-antibody complex and preparation and application of polypeptide
  • Polypeptide, polypeptide derivative, and polypeptide-antibody complex and preparation and application of polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1 Polypeptide Derivative Synthesis

[0083] The polypeptide derivative of the present invention utilizes the Fmoc solid-phase polypeptide synthesis technology to complete the peptide chain, and the purity is greater than 95% through HPLC detection.

[0084] Synthetic raw materials and related reagents:

[0085] Fmoc-L-Asp(Otbu)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0086] Fmoc-L-Lys(Boc)-OH, Jill Biochemical (Shanghai) Co., Ltd.

[0087] Fmoc-L-Gly-OH, Jill Biochemical (Shanghai) Co., Ltd.

[0088] Fmoc-Pro-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0089] Fmoc-Ser(Tbu)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0090] Fmoc-L-Lys(Dde)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0091] myristic acid,

[0092] Azidoacetic acid NHS active ester

[0093] HBTU (N, N, N', N'-tetramethyl-O-(1H-benzotriazole) urea hexafluorophosphate),

[0094] DIEA (N, N-diisopropylethylamine), DMF (N, N-dimethylformamide),

[0095] DCM (dichloromethane), acetonitril...

Embodiment 2

[0145] Example 2 Synthesis of Polypeptide Derivatives

[0146] The peptide chain is completed by Fmoc solid-phase peptide synthesis technology, and the purity is greater than 95% through HPLC detection.

[0147] Synthetic raw materials and related reagents are the same as in Example 1.

[0148] Fmoc-L-Asp(Otbu)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0149] Fmoc-L-Lys(Boc)-OH, Jill Biochemical (Shanghai) Co., Ltd.

[0150] Fmoc-L-Gly-OH, Jill Biochemical (Shanghai) Co., Ltd.

[0151] Fmoc-Pro-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0152] Fmoc-Ser(Tbu)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0153] Fmoc-L-Lys(Dde)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0154] Cyclooctyne NHS active ester:

[0155] Solid-phase peptide synthesis operation steps:

[0156] 1. Resin swelling

[0157] Weigh 1.0gAM Resin resin with a substitution degree of 0.4mmol / g, put the resin into a reaction tube, add DCM (18ml / g), and shake for 50min.

[0158] 2. Take the first amino...

Embodiment 3

[0202] Example 3 Polypeptide Derivative Synthesis

[0203] The peptide chain is completed by Fmoc solid-phase peptide synthesis technology, and the purity is greater than 95% through HPLC detection.

[0204] Synthetic raw materials and related reagents are the same as in Example 1.

[0205] Fmoc-L-Asp(Otbu)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0206] Fmoc-L-Lys(Boc)-OH, Jill Biochemical (Shanghai) Co., Ltd.

[0207] Fmoc-L-Gly-OH, Jill Biochemical (Shanghai) Co., Ltd.

[0208] Fmoc-Pro-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0209] Fmoc-Ser(Tbu)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0210] Fmoc-L-Lys(Dde)-OH, Gill Biochemical (Shanghai) Co., Ltd.

[0211] Solid-phase peptide synthesis operation steps:

[0212] 1. Resin swelling

[0213] Weigh 1.0gAM Resin resin with a substitution degree of 0.4mmol / g, put the resin into a reaction tube, add DCM (18ml / g), and shake for 50min.

[0214] 2. Take the first amino acid

[0215] Filter the solvent through the...

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Abstract

The invention discloses a polypeptide, and belongs to the field of biomedicine. In order to solve the problems of complicated production process, high cost and low targeting existing in existing methods for enhancing the targeting of T cells, the invention provides the polypeptide. The polypeptide can significantly enhance the targeting of immune cells. At the same time, the preparation process ofthe polypeptide is simple, and the cost is lower than the existing cost of preparing CAR-T cells or the existing cost of preparing bispecific antibody cells.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to polypeptides, polypeptide derivatives, polypeptide-antibody complexes and their preparation and application. Background technique [0002] At present, tumors have become one of the serious diseases that endanger human health. According to the 2017 China Cancer Registration Annual Report, about 10,000 people are diagnosed with tumors every day, and about 7 people suffer from cancer every minute. Compared with 2012, the number of new cancers In the same year, the number of new cancer cases in the world was 14.09 million, and China's new cancer cases accounted for about 1 / 4 of the world's total. At present, the treatment methods for tumors are mainly divided into surgical resection, radiotherapy, chemotherapy and immunotherapy, etc. Immunotherapy is getting more and more attention. There are currently two ways to enhance the targeting of T cells, as follows: [0003] Chimeric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K14/00C07K16/00C12N5/078A61K47/42A61K39/395A61K35/17A61K47/64A61P35/00A61P35/02
CPCA61K35/17A61K39/39558A61K47/42A61K47/64A61P35/00A61P35/02C07K7/08C07K14/00C07K16/00C07K19/00C12N5/0634
Inventor 吴疆刘丹邓蒙蒙王保如
Owner 安徽瑞达健康产业有限公司
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