Bacillus aryabhattai as well as metabolite and application thereof
A technology of Bacillus arborii and its metabolites, applied in the fields of application, bacteria, and microorganisms, can solve the problems of environmental friendliness and unsatisfactory control effects of plant wilt prevention and control, so as to ensure the healthy and sustainable development of agriculture, Inhibition effect, effect of broad application prospects
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Embodiment 1
[0027] Example 1: Screening and Identification of Biocontrol Strains
[0028] (1) screening
[0029] In December 2018, Wang Enzhao collected the healthy rice rhizosphere soil in the pot experiment in the greenhouse of the Chinese Academy of Agricultural Sciences, that is, removed about 5 cm of the covering soil on the root surface from the rice, and randomly collected the loose soil combined with the rice root system in an S shape.
[0030] The control group was sterile water, and the test group was soil sample suspension with gradient dilution.
[0031] The specific operation method of the test group is as follows:
[0032] Use the gradient dilution coating method to apply the suspension of the soil sample diluted step by step on the beef extract peptone plate, pick a single colony into the liquid beef extract peptone medium to obtain the bacterial liquid, and take each bacterial liquid and spread it evenly on the beef extract peptone plate. Cultivate on paste peptone mediu...
Embodiment 2
[0046] Embodiment 2: the identification of Bacillus arborii antibacterial metabolites
[0047] Volatile organic compounds are solids or liquids with carbon as the basic element, which can quickly volatilize at 20°C and 0.01 kPa and enter the gas phase. Based on the inventor's long-term accumulated research experience in the early stage, studies have confirmed that such microorganisms can produce a large number of volatile organic compounds with rich types and diverse functions. The main functions of these substances are: as signal substances between communities and within communities; Signaling substances among them; possible carbon release channels; growth-promoting and growth-inhibiting factors. If they function as inhibitors and the phytopathogenic microorganisms are inhibited in the interaction, then the microorganisms releasing volatile organic compounds have the potential to be applied to the biological control of plant diseases.
[0048] (1) Collection of volatile organi...
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