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Method for preparing polypeptide-coated anthocyanin microspheres based on microfluid technology and application of polypeptide-coated anthocyanin microspheres

An anthocyanin and microfluidic technology, which is applied in applications, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of anthocyanin denaturation, inability to effectively isolate air oxidation, etc., and achieve easy absorption, green production process, and biological activity high effect

Inactive Publication Date: 2019-12-13
扬州博科文化发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot effectively isolate air oxidation, and high temperature is more likely to cause denaturation of anthocyanins

Method used

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  • Method for preparing polypeptide-coated anthocyanin microspheres based on microfluid technology and application of polypeptide-coated anthocyanin microspheres
  • Method for preparing polypeptide-coated anthocyanin microspheres based on microfluid technology and application of polypeptide-coated anthocyanin microspheres
  • Method for preparing polypeptide-coated anthocyanin microspheres based on microfluid technology and application of polypeptide-coated anthocyanin microspheres

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Experimental program
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Effect test

Embodiment 1

[0045] A method for preparing polypeptide-wrapped anthocyanin microspheres based on microfluidic technology, the preparation steps are as follows:

[0046] (1) Drawing: use the engineering drawing software AutoCAD to design the microfluidic pipeline, which has a water phase inlet, an oil phase inlet, and a product collection outlet. The diameter of the inlet and outlet is 1 mm, the depth of the pipeline is 50 microns, and the width is 100 microns. ; The structure of the microfluidic pipeline is as Figure 5 shown;

[0047] (2) Mold making: The lithography template obtained in step (1) is projected and printed on the silicon wafer substrate in equal proportions using soft lithography technology, with a size ratio of 1:1. The pipeline is made of SU-8 2100 photoresist, the depth of the pipeline is 50 microns, and the width is controlled to 100 microns;

[0048] (3) Remove the mold: After mixing the dimethylsiloxane monomer and the curing agent in a ratio of 10:1, pour it on the...

Embodiment 2

[0054] A method for preparing polypeptide-wrapped anthocyanin microspheres based on microfluidic technology, the preparation steps are as follows:

[0055] (1) Tube making: We used a round glass capillary (CM Scientific) with a length of 2 cm, an inner diameter of 0.5 mm, and an outer diameter of 0.7 mm as the inner tube, and used a capillary stretching machine (P-97, Sutter Instrument Company ) was stretched at one end to form a streamlined constriction port with a port size of 0.1 mm; we used another square glass capillary (CM Scientific) with a length of 5 cm, an inner side of 0.7 mm and an outer side of 1 mm as the outer tube. The inner and outer tubes are fully cleaned and dried before use.

[0056] (2) Assembly: Carefully insert the inner tube obtained in step (1) into the outer tube by about 5 mm, and connect the distal end of the inner tube, the junction of the inner and outer tubes, and the distal end of the outer tube to the engineering plastic adapter (Nanoports), r...

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Abstract

The invention discloses a method for preparing polypeptide-coated anthocyanin microspheres based on a microfluid technology and an application of the polypeptide-coated anthocyanin microspheres. The method comprises the following steps: a microfluidic chip is prepared; an aqueous solution in which anthocyanin and polypeptide are co-dissolved and an ethyl acetate solution are respectively fed froman aqueous phase inlet and an oil phase inlet respectively, and by adjustment of the flow quantity ratio of a water phase flow quantity to an oil phase flow quantity, a uniform and controllable anthocyanin emulsion is obtained; the anthocyanin emulsion is injected into the microfluidic chip, and polypeptide plays a role of a surfactant and also is used as a wrapping layer to wrap the anthocyanin emulsion; a mixture of the obtained anthocyanin emulsion and ethyl acetate is collected, stood and separated, the ethyl acetate solution on the upper layer is skimmed, the anthocyanin emulsion is transferred into a freeze dryer, ultralow-temperature drying is carried out at the temperature of -80 DEG C, and thus the polypeptide-coated anthocyanin microspheres are obtained. The amphiphilic polypeptide is used as a wrapping material, the mulberry anthocyanin is wrapped by combining the microfluid emulsification technology, the stability of the obtained microspheres is high, and the application field of the obtained anthocyanin is wide.

Description

technical field [0001] The invention belongs to the technical field of biological material application, and in particular relates to a method for preparing polypeptide-wrapped anthocyanin microspheres based on microfluidic technology and its application. Background technique [0002] Anthocyanins are a kind of water-soluble natural pigments that widely exist in plants in nature, and are colored aglycones obtained by hydrolyzing anthocyanins. Anthocyanins have nutritional and multiple health functions, and have broad application prospects in the food, drug and cosmetic industries. However, anthocyanins have low stability and are easily degraded; they are greatly affected by factors such as temperature, pH, and light. These factors will accelerate the degradation of anthocyanins, limiting the application of anthocyanins in various industries. Therefore, how to improve the stability of anthocyanins is the bottleneck to expand its application. Methods to improve the stabili...

Claims

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Application Information

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IPC IPC(8): A61K8/11A61K8/49A61Q19/00A23L29/00A23L33/00A23L33/18
CPCA61K8/11A61K8/498A61Q19/00A23L33/00A23L33/18A23L29/045A23L29/035A61K2800/10A23V2002/00A23V2200/30A23V2250/2104A23V2250/55
Inventor 徐江张磊张沁榕桂仲争
Owner 扬州博科文化发展有限公司