Method for microwave extraction of ursolic acid and oleanolic acid in loquat leaves

A technology of oleanolic acid and ursolic acid, applied in the direction of steroids, organic chemistry, etc., can solve the problems of long extraction time and large amount of solvent, and achieve the effect of improving purity, increasing extraction rate, and ensuring accuracy

Inactive Publication Date: 2019-12-13
广东科贸职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The methods for extracting ursolic acid and oleanolic acid from loquat leaves mainly include cold soaking method, reflux extraction method, ultrasonic-assisted extraction method, Soxhlet extraction method, etc., but the extraction rate is generally 92%-93.56%, and the extraction time Long, large amount of solvent

Method used

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  • Method for microwave extraction of ursolic acid and oleanolic acid in loquat leaves
  • Method for microwave extraction of ursolic acid and oleanolic acid in loquat leaves
  • Method for microwave extraction of ursolic acid and oleanolic acid in loquat leaves

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Experimental program
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Effect test

Embodiment 1

[0053] Step 1, dry and pulverize loquat leaves, pass through a 30-mesh sieve, place in a microwave reflux extraction device, add an ethanol solution with a mass concentration of 50%, and extract the first extract and the first filtrate through microwave-assisted reflux extraction; microwave extraction The temperature is 40°C, the microwave extraction time is 60min; the microwave power is 200w.

[0054] Step 2, take the first filtrate and add ethanol solution with a mass concentration of 50% again, and carry out reflux extraction to obtain a second extract; the extraction temperature is 50° C., and the extraction time is 50 min.

[0055] Step 3. Combine the first extract and the second extract to obtain a mixed extract, add a sodium hydroxide solution with a mass concentration of 5%, adjust the pH value of the mixed extract to 8, heat in a water bath for 10 minutes, and filter to obtain the second extract. a filtrate.

[0056] Step 4. Add sulfuric acid solution with a mass con...

Embodiment 2

[0060] Step 1, dry and pulverize loquat leaves, pass through a 40-mesh sieve, place in a microwave reflux extraction device, add an ethanol solution with a mass concentration of 85%, and obtain the first extract and the first filtrate through microwave-assisted reflux extraction; microwave extraction The temperature is 90°C, the microwave extraction time is 15min; the microwave power is 500w.

[0061] Step 2, taking the first filtrate and adding an ethanol solution with a mass concentration of 85% to carry out reflux extraction to obtain a second extract; the extraction temperature is 95° C., and the extraction time is 20 minutes.

[0062] Step 3. Combine the first extract and the second extract to obtain a mixed extract, add a sodium hydroxide solution with a mass concentration of 5%, adjust the pH value of the mixed extract to 9, heat in a water bath for 12 minutes, and filter to obtain the second extract. a filtrate.

[0063] Step 4. Add sulfuric acid solution with a mass ...

Embodiment 3

[0067] Step 1, dry and pulverize loquat leaves, pass through a 50-mesh sieve, place in a microwave reflux extraction device, add an ethanol solution with a mass concentration of 95%, and obtain the first extract and the first filtrate through microwave-assisted reflux extraction; microwave extraction The temperature is 60°C, the microwave extraction time is 15min; the microwave power is 300w.

[0068] Step 2, take the first filtrate and add the ethanol solution with a mass concentration of 95% again, and carry out reflux extraction to obtain the second extraction solution; the extraction temperature is 70° C., and the extraction time is 40 minutes.

[0069] Step 3. Combine the first extract and the second extract to obtain a mixed extract, add a sodium hydroxide solution with a mass concentration of 5%, adjust the pH value of the mixed extract to 11, heat in a water bath for 15 minutes, and filter to obtain the second extract. a filtrate.

[0070] Step 4. Add sulfuric acid so...

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Abstract

The invention discloses a method for microwave extraction of ursolic acid and oleanolic acid in loquat leaves. The method comprises the following steps: step 1, putting loquat powder into a microwavereflux extraction device, adding an ethanol solution, and carrying out microwave-assisted reflux extraction; step 2, carrying out secondary reflux extraction to obtain a second extract; 3, mixing first extract and the second extract, adding a sodium hydroxide solution, adjusting a pH value, carrying out heating in a water bath, and performing filtering to obtain a first filtrate; step 4, adding asulfuric acid solution into the first filtrate, adjusting the pH value, performing diluting with an acidic aqueous solution with an equal volume and an identical pH value, and carrying out heating ina water bath to obtain crude extract of ursolic acid and oleanolic acid; step 5, performing separating to obtain a high-purity ursolic acid sample and a high-purity oleanolic acid sample; and 6, performing drying, and carrying out content determination by using high-performance liquid chromatography to obtain ursolic acid and oleanolic acid both with purity of 96% or above, wherein the yield of ursolic acid is 3.1 mg / g or above and the yield of oleanolic acid is 0.48 mg / g or above. According to the method, the purity and the extraction rate of ursolic acid and oleanolic acid are effectively improved, operation is time-saving, and cost consumption is reduced.

Description

technical field [0001] The invention relates to the technical field of extraction of effective components of plants, in particular to a method for microwave extraction of ursolic acid and oleanolic acid in loquat leaves. Background technique [0002] Loquat leaf is the leaf of Rosaceae plant Loquat. It is cultivated in most areas of my country and can be harvested throughout the year. It has the functions of clearing lung and relieving cough, reducing adverse reactions and relieving vomiting. It is a traditional Chinese medicine commonly used. The active ingredients in loquat leaves include volatile oil, triterpene acids, flavonoids, saponins, organic acids, polyphenols, etc. Pharmacological research shows that its main active ingredient is triterpene acid compound, and the triterpene acid compound obtained from Loquat Leaf is mainly ursolic acid, oleanolic acid and parent derivatives thereof at present. [0003] Ursolic acid is a triterpenoid compound present in natural pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 黄百祺吴巨贤何敏戴远威朱华
Owner 广东科贸职业学院
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