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A kind of immobilized metal ion-magnetic liposome and its preparation method and application

A technology for magnetic liposomes and immobilized metals, applied in the field of liposomes, can solve problems such as low separation efficiency, and achieve the effects of improving separation efficiency, high selectivity and good stability

Active Publication Date: 2021-01-01
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biomagnetic separation technology of active protein requires complex pretreatment operations such as filtration and centrifugation of the sample solution, and the separation efficiency is low.

Method used

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  • A kind of immobilized metal ion-magnetic liposome and its preparation method and application
  • A kind of immobilized metal ion-magnetic liposome and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] An immobilized metal ion-magnetic liposome, composed of phospholipids and N-alkyliminodiacetic acid wrapped magnetic nano ferric iron tetroxide to form a macromolecular liposome with a phospholipid bilayer membrane structure, and behind the macromolecular lipid Loading and immobilizing transition metal ions on the surface of the plastid.

[0026] The preparation method of described immobilized metal ion-magnetic liposome, comprises the steps:

[0027] Weigh 1.3661g FeCl respectively 3 ·6H 2 O and 0.5334 g FeCl 2 4H 2 O(Fe 3+ with Fe 2+ The molar ratio is 2:1), dissolved in 100ml deionized water, stirred thoroughly to mix, and transferred to a 500ml three-neck flask. Then place it in a constant temperature water bath at 30°C, under nitrogen protection, with strong mechanical stirring, and add 10 mL of NH 3 ·H 2 O, make the pH of the solution about 8.5, add 50 mL of 0.5% (w / v) lecithin-ethanol solution after reacting for 30 min. At this time, a large number of bl...

Embodiment 2

[0031] A kind of preparation method of immobilized metal ion-magnetic liposome, comprises the steps:

[0032] Weigh 1.3661g FeCl respectively 3 ·6H 2 O and 0.5334 g FeCl 2 4H 2 O(Fe 3+ with Fe 2+ The molar ratio is 2:1), dissolved in 100ml deionized water, fully stirred to mix, and transferred to a 500ml three-neck flask. Then placed in a constant temperature water bath at 30°C, under nitrogen protection, with strong mechanical stirring, 10 mL of NH 3 ·H 2O, make the pH of the solution about 8.5, add 70 mL of 1% (w / v) lecithin-ethanol solution after reacting for 30 min. At this time, a large number of black particles were formed in the solution, and the temperature was raised to 90°C. Add 1 mol / L NaOH dropwise, adjust the pH to 12, and stir for 30 min. After the end, under the action of an external magnetic field, the prepared black particles are separated and washed with a large amount of deionized water, that is, the magnetic liposome is ready for use.

[0033] Wei...

Embodiment 3

[0035] A kind of preparation method of immobilized metal ion-magnetic liposome, comprises the steps:

[0036] Weigh 1.3661g FeCl respectively 3 ·6H 2 O and 0.5334 g FeCl 2 4H 2 O(Fe 3+ with Fe 2+ The molar ratio is 2:1), dissolved in 100ml deionized water, fully stirred to mix, and transferred to a 500ml three-neck flask. Then placed in a constant temperature water bath at 30°C, under nitrogen protection, with strong mechanical stirring, 10 mL of NH 3 ·H 2 O, make the pH of the solution about 8.5, add 60 mL of 1.5% (w / v) lecithin-ethanol solution after reacting for 30 min. At this time, a large number of black particles were formed in the solution, and the temperature was raised to 90°C. Add 1 mol / L NaOH dropwise, adjust the pH to 12, and stir for 30 min. After the end, under the action of an external magnetic field, the prepared black particles are separated and washed with a large amount of deionized water, that is, the magnetic liposome is ready for use.

[0037] ...

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Abstract

The invention discloses an immobilized metal ion-magnetic liposome as well as its preparation method and application. Metal ion-magnetic liposome of the present invention is wrapped magnetic nano iron ferric oxide by phospholipid, N-hexadecyl iminodiacetic acid, after forming the macromolecule liposome with phospholipid bilayer membrane structure, and on its surface Loading and immobilization of transition metal ions. Different from the traditional magnetic liposome preparation method, the present invention adopts in situ reaction method to prepare magnetic liposome, the main method is to make FeCl 3 ·6H 2 O and FeCl 2 4H 2 The O solution is reacted by adding ammonia water dropwise under a certain temperature, high-speed stirring, and nitrogen protection, and then adding phospholipid-ethanol solution to obtain magnetic liposomes, and then coupling transition metal ions. The immobilized metal ion-magnetic liposome of the present invention has dual functions of imitating cell membrane affinity adsorption and metal ion affinity adsorption, and has a simple preparation method and good stability.

Description

technical field [0001] The invention relates to the technical field of liposomes, in particular to an immobilized metal ion-magnetic liposome and its preparation method and application. Background technique [0002] In the 21st century, with the rapid development of life sciences, the rapid and efficient separation of biological macromolecules using new technologies has attracted more and more attention. Biomagnetic separation technology is a new separation method developed on the basis of traditional magnetic separation technology. It is an efficient separation technology for organisms such as cells, bacteria, nucleic acids and proteins. It has the advantages of high efficiency, rapidity and simplicity; It uses the characteristic of magnetic or magnetically labeled organisms to move directionally under the action of an external magnetic field to realize the extraction, enrichment, separation and purification of target organisms. [0003] At present, the immobilized metal i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01F1/00H01F41/00B01D15/38C07K1/22
CPCB01D15/3804C07K1/22H01F1/0054H01F41/00
Inventor 伍善广陆苑冯学珍蒙华琳陆玉婷韦啟球廖娜
Owner GUANGXI UNIVERSITY OF TECHNOLOGY