A kind of culture medium group and its application of the tissue culture rapid propagation of the plant

Abstract

The invention relates to the technical field of plant cell induction and tissue culture, and discloses a culture medium group for tissue culture and rapid propagation of plants of the genus Cymbidium, including an induction medium, a differentiation medium and a rooting medium, and the induction medium includes 0.8 ~1.5MS medium, 6‑BA 0.5~1.0mg / L, IAA 0.1~0.3mg / L, 2,4‑D 0.1~0.2mg / L, Vc 0.2~0.4mg / L, nitrosoferricyanide Sodium 2.0~6.0mg / L, sucrose 25~30g / L and agar 5~7g / L, pH=5.6~6.2; the differentiation medium includes 0.8~1.5MS medium, 6-BA 0.5~1.0mg / L , NAA 0.05~0.1mg / L, IAA 0.05~0.1mg / L, sucrose 25~30g / L and agar 5~7g / L, pH=5.6~6.2; The rooting medium includes 0.5~1.0MS medium, NAA 0.1~0.2mg / L, IBA 0~0.05mg / L, sucrose 15~25g / L, agar 5~7g / L and potato juice 1.0~3.0mL / L; the invention also discloses the application of the medium group , the medium group achieves the effect of high-efficiency breeding of cultivated seedlings of the genus Cymbidium through the targeted limitation of the medium components and ratios of the multi-phytohormone combination.

Description

technical field [0001] The invention relates to the technical field of plant cell induction and tissue culture, in particular to a culture medium group for tissue culture and rapid propagation of plants of the genus Cymbidium and its application. Background technique [0002] Dracocephalum belongs to Labiatae, herbaceous, perennial, with woody rhizomes, and rarely annual. Stems often arise from rhizomes, erect, sparsely spread, often unbranched or with few branches, sparsely numerous branches, quadrangular. Leaves opposite, basal leaves with long stalks, cauline leaves with short stalks or sessiles, often heart-shaped ovate or oblong, or lanceolate, margins crenate or serrate, or entire, usually not Divided or pinnately nearly palmately parted. Verticels densely clustered into heads or spikes or sparsely arranged; flowers usually blue-purple, rarely white; bracts often obovate, often with sharp teeth or spines, rarely entire. The phytochemical composition of the genus Qin...

AI Technical Summary

Problems solved by technology

[0017] However, at present, when using the phytohormone ratio in the conventional plant tissue culture medium to breed the plants of the genus Cymbidium, the efficiency is very low, even not as high as the seed propagation efficiency

In addition, due to the small size of Maojiancao seeds, it is not easy to collect, and the seed germination rate is not high in the natural environment, which makes it difficult to reproduce the seeds; at the same time, because of its rich secondary metabolites, it contains a variety of enzymes and various small Molecular substances have seriously affected the effect of the phytohormone combination of conventional plant tissue culture, so that the conventional plant tissue culture method can not effectively achieve the efficiency of tissue culture for the breeding of Maojiancao

For example, the commonly used media for tobacco tissue culture cannot complete the regeneration of plants of the genus Pseudomonas genus, such as Maojiancao, Minshan Maojiancao, and Songye Qinglan. The induction medium cannot induce enough callus tissue. During the induction process, the tissue The browning of the material is also obvious

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