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Bactrocera cucurbitae solid culture medium and preparation method thereof

A solid medium and medium technology, applied in the field of bioengineering, can solve the problems of difficult detection of fruit fly resistance, uneven nutrient distribution, low hatching rate of offspring, etc., to solve the technical problems of artificial mass breeding, Significant economic and social benefits, the effect of promoting research and development

Pending Publication Date: 2019-12-27
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few studies on the solid culture medium of the fruit fly larvae. The existing solid culture medium is a feed mixed with solid powder, and mostly contains toilet paper, which makes the feed difficult to mix evenly, takes a lot of work and time, and is easily contaminated by microorganisms. The cost is high and cannot be promoted
And the inhomogeneous distribution of nutrients in the feed is also one of the factors that cause the hatching rate of offspring of B.
At the same time, the feed is mixed with solid powder and the feed is not evenly mixed, which also makes it difficult to detect the resistance to B.

Method used

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  • Bactrocera cucurbitae solid culture medium and preparation method thereof
  • Bactrocera cucurbitae solid culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The solid medium for fruit fly larvae includes 5-25wt% pumpkin, 5-20wt% yeast powder, 0.25-1wt% yeast extract, 1.5-6wt% soybean meal, 3.5-15wt% corn meal, 1 -5wt% glucose, 2-10wt% white sugar, 0.025-0.1wt% calcium chloride, 0.25-1wt% agar, 0.3-1.2% propionic acid, and appropriate amount of water.

[0032] According to an embodiment of the present application, the solid medium for fruit fly larvae includes 8 wt% pumpkin, 8 wt% yeast powder, 0.4 wt% yeast extract, 2.4 wt% soybean meal, 5.2 wt% corn meal, 2wt% glucose, 4.8wt% white sugar, 0.04wt% calcium chloride, 0.44wt% agar, 0.48% by volume of propionic acid, and appropriate amount of water.

[0033] For example, according to an embodiment of the present application, to make 250 mL of fruit fly larvae solid culture medium, 20g pumpkin, 13g corn meal, 20g yeast powder, 12g white sugar, 5g glucose, 6g soybean meal, 1g yeast extract, and chlorinated Calcium 0.1g, agar 1.1g, propionic acid 1.2mL, add water, dilute the solution ...

Embodiment 2

[0035] The preparation method of solid culture medium for fruit fly larvae includes the following steps:

[0036] 1. Weigh the pumpkin according to the amount of medium required. The preparation of the medium for this application does not limit the variety and size of the added pumpkin. According to an embodiment of the present application, a mature pumpkin can be used, which has richer nutrients. According to an embodiment of the present application, the pumpkin can be boiled in boiling water for 5-20 minutes and then removed. This process can not only sterilize the pumpkin, but also reduce the hardness of the pumpkin, which is convenient for subsequent medium preparation. According to another embodiment of the present application, untreated pumpkin can also be used directly.

[0037] Make pumpkin puree and place in a container for later use. According to an embodiment of the present application, a food processor, a juicer, a wall breaker, etc. can be used to prepare pumpkin p...

Embodiment 3

[0045] The fruit fly solid medium prepared by the preparation method of the present invention is used for larval rearing, the newly hatched fruit fly larvae are connected to the solid medium for fruit fly, and the larvae are reared for about 7 days.

[0046] As shown in Table 1 and Table 2, Table 1 is the formula table of the solid medium for fruit fly larvae; Table 2 is the comparison of the effect of feeding larvae on the solid medium for fruit fly larvae of different formulas. Among them, the first line is the materials or solutions that may be used when configuring the culture medium. The experimental groups A, B, and C are according to the examples of this application, the amount of each substance added is fine-tuned, and the formula of the medium is configured. CK1 is the control group. For the fruit fly larva medium formula used before the transformation of this application, CK2 is the control group, and pumpkin is the natural food for fruit fly. This application combines ...

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Abstract

The invention relates to a bactrocera cucurbitae solid culture medium. The bactrocera cucurbitae solid culture medium is prepared from the following components in percentage by weight: 5-25% of a pumpkin, 1-15% of a carbon source, 10-46% of a nitrogen source, 0.025-0.1% of a calcium source, 0.25-1% of a curing agent, and the balance of water, wherein the carbon source comprises one or more of monosaccharide, disaccharide or polysaccharide, the nitrogen source comprises yeast powder and / or cereal flour, and the curing agent is agar and / or agarose. The bactrocera cucurbitae larva solid culture medium is simple, efficient, economic, applicable, easy to operate, and capable of saving labor, and meanwhile, can be effectively quantified, the technical problem of indoor manual mass rearing of bactrocera cucurbitae is thoroughly solved, the research and development of the bactrocera cucurbitae solid culture medium in China is promoted extremely significantly, a large number of insect sources are provided for various scientific research of the bactrocera cucurbitae in the future, and the significant economic and social benefits are achieved.

Description

Technical field [0001] The invention relates to the field of bioengineering, in particular to a solid culture medium for fruit fly larvae and a preparation method thereof. Background technique [0002] Bactrocera (Zeugodacus) cucurbitae (Coquillett), belongs to Diptera (Diptera), Tephritidae (Tephritidae), Zeugodacus (Zeugodacus). Fruit fly is an important pest of vegetables and fruits. It has a wide range of hosts and is highly harmful, including more than 100 kinds of vegetables and fruits such as pumpkin, loofah, bitter gourd, citrus, mango and guava, which can cause up to 30%-100% loss. [0003] Bactrocera cucurbita is listed as an entry plant quarantine pest and an invasive alien species under key management. It originated in India and is widely distributed in tropical and subtropical countries and regions. Bactrocera cucurbitae can safely survive the summer in all parts of China. The northern boundary of the distribution of Bactrocera cucurbitae that cannot overwinter in C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/90A23K10/30A23K20/163A23K10/16A23K20/24A23K20/105
CPCA23K10/16A23K10/30A23K20/105A23K20/163A23K20/24A23K50/90
Inventor 颜日辉刘向蕊张积李周容刘中更刘焱晖马思雅李静姜迦荣姜迦淞颜新华任玉林冯舒文郉增珠
Owner HAINAN UNIVERSITY
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