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A kind of tiger fish ptscd1 gene and its application

A tiger skin and gene sequence technology, applied in the tiger skin fish PtScd1 gene and its application field, can solve problems such as energy waste, achieve the effect of improving low temperature tolerance and improving low temperature survival rate

Active Publication Date: 2021-01-22
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production and breeding process, fish farms usually rely on geothermal or coal combustion to maintain a suitable water temperature; in the end consumer market, ornamental fish lovers usually use heating rods to keep the water temperature of tiger fish constant, resulting in a huge waste of energy

Method used

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  • A kind of tiger fish ptscd1 gene and its application
  • A kind of tiger fish ptscd1 gene and its application
  • A kind of tiger fish ptscd1 gene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0014] 1. Materials

[0015] 1. Tiger fish farming

[0016] Tiger skin fish has been cultured for a long time after domestication. The breeding conditions are: water temperature 25-27°C, pH 6.5-7.4, kH 6-7. Feed 2 times, and absorb the feed residue after feeding for 10 minutes.

[0017] 2. Gradient low temperature stress test

[0018] A total of 150 healthy and lively 8-month-old tiger fish with similar body size (no distinction between male and female) were selected for the gradient low temperature stress test. 150 tiger skin fishes are randomly divided into 5 culture tanks, each culture tank is 30L, containing 30 tiger skin fishes. The water temperature was maintained at 27°C on the first day of the test, and the temperature was lowered every 24 hours from the next day as follows: △T1=4°C / d, △T2=4°C / d, △T3=4°C / d, △T4=2°C / d, Other conditions remain unchanged. After each temperature gradient was maintained for 24h, 3 tiger skin fishes ( figure 1 ), ethyl m-aminobenzoate ...

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Abstract

The invention discloses a puntius tetrazona PtScd1 gene and application thereof, and belongs to the technical fields of biochemistry and molecular biology. According to the puntius tetrazona PtScd1 gene and application thereof, through RACE cloning and sequence assembly, a puntius tetrazona PtScd1 cDNA sequence is obtained, and it is predicted that an ORF sequence contains 981 nucleotides, and codes for 326 animo acid; and the animo acid sequence contains a highly-conserved fatty acid desaturase structural domain, double iron ion binding sites exist in a protein three-dimensional structure, and can bind substrates ST9 and MPG. A qPCR technology detects that PtScd1 mRNA is expressed in the brain, the gills, the liver and muscle tissues of puntius tetrazona, wherein the expression level of the liver is the highest; and rising of the level of the PtScd1 mRNA of puntius tetrazona is induced by low temperature, and a relative expression level of the PtScd1 mRNA of brain tissues and the temperature present significant negative correlation. The PtScd1 is screened out as a low temperature-resistant gene of puntius tetrazona.

Description

technical field [0001] The invention belongs to the technical field of biochemistry and molecular biology, and specifically relates to a tiger skin fish PtScd1 gene and an application thereof. Background technique [0002] Stearoyl-CoA desaturase (stearoyl-CoA desaturase, SCD) is a kind of acyl-CoA, which is the rate-limiting enzyme of monounsaturated fatty acid synthesis pathway. play an important role in the process. SCD is widely found in unicellular organisms, mammals and humans, and the enzyme activity and gene expression of SCD have also been reported in many aquatic animals, including carp (Cyprinus carpio), zebrafish (Danio rerio), tilapia (Oreochromis mossambicus ) Grass carp (Ctenopharyngodon idella, Megalobrama amblycephala), turbot (Scophthalmus maximus), yellow catfish (Pelteobagrus fulvidraco), octopus (Octopus vulgaris), large yellow croaker (Larimichthys crocea), etc. The fatty acid composition and SCD of fish (Chanos chanos) hepatic cell membranes changed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12N15/53C12Q1/6888
CPCC12N9/0071C12Q1/6888C12Q2600/124C12Q2600/158C12Y114/19001
Inventor 刘丽丽朱华张蓉王晓雯
Owner BEIJING FISHERIES RES INST
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