Preparation method of lovastatin-rich functional red yeast rice
A lovastatin and functional technology, which is applied in the field of preparation of lovastatin-rich functional red yeast rice, can solve the problem of low lovastatin content, achieve increased lovastatin content, simple process operation, and favorable growth metabolic effect
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Embodiment 1
[0029] A preparation method rich in lovastatin functional red yeast rice, which comprises the steps of:
[0030] 1) Rice soaking: using indica rice as raw material, soak the indica rice in glacial acetic acid aqueous solution with a mass concentration of 0.2% for 4 hours, then pick up and drain, and collect for later use;
[0031] 2) High temperature sterilization: put 30g of indica rice treated in step (1) into a 250mL triangular flask, then add water, the weight ratio of the added water to rice is 0.4:1kg / kg, and then sterilize at 121°C under high temperature Bacteria, the sterilization time is 20min, and then placed to cool to room temperature, that is, prepared into a solid fermentation medium and set aside;
[0032] 3) Seed liquid preparation: After the Monascus test tube is activated, transfer it to a 90mm PDA plate medium for cultivation. The cultivation time is 4 days and the cultivation temperature is 32°C. , scraping the hyphae to obtain the Monascus suspension;
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Embodiment 2
[0037] A preparation method rich in lovastatin functional red yeast rice, which comprises the steps of:
[0038] 1) Rice soaking: using indica rice as raw material, soak the indica rice in glacial acetic acid aqueous solution with a mass concentration of 0.2% for 4 hours, then pick up and drain, and collect for later use;
[0039] 2) High temperature sterilization: put 30g of indica rice treated in step (1) into a 250mL triangular flask, then add water, the weight ratio of the added water to rice is 0.4:1kg / kg, and then sterilize at 121°C under high temperature Bacteria, the sterilization time is 20min, and then placed to cool to room temperature, that is, prepared into a solid fermentation medium and set aside;
[0040]3) Seed liquid preparation: After the Monascus test tube is activated, transfer it to a 90mm PDA plate medium for cultivation. The cultivation time is 4 days and the cultivation temperature is 32°C. , scraping the hyphae to obtain the Monascus suspension;
[...
Embodiment 3
[0045] Detection of lovastatin in functional red yeast rice: Lovastatin standard product is Lovastatin (L0790000, sigma), according to the "People's Republic of China Light Industry Standards" QB / T 2487-2007 Annex A functional red yeast rice The content detection of lovastatin in the present invention is carried out by means of determination of Monacolin K content.
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