Preparation method of lovastatin-rich functional red yeast rice

A lovastatin and functional technology, which is applied in the field of preparation of lovastatin-rich functional red yeast rice, can solve the problem of low lovastatin content, achieve increased lovastatin content, simple process operation, and favorable growth metabolic effect

Pending Publication Date: 2019-12-31
湖南微著生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the content of lovastatin in functional red yeast rice is generally low in the production of functional red yeast rice by traditional solid-state fermentation technology with bulk grain rice as the substrate, the present invention provides a preparation technology for preparing functional red yeast rice rich in lovastatin

Method used

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  • Preparation method of lovastatin-rich functional red yeast rice
  • Preparation method of lovastatin-rich functional red yeast rice

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Experimental program
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Effect test

Embodiment 1

[0029] A preparation method rich in lovastatin functional red yeast rice, which comprises the steps of:

[0030] 1) Rice soaking: using indica rice as raw material, soak the indica rice in glacial acetic acid aqueous solution with a mass concentration of 0.2% for 4 hours, then pick up and drain, and collect for later use;

[0031] 2) High temperature sterilization: put 30g of indica rice treated in step (1) into a 250mL triangular flask, then add water, the weight ratio of the added water to rice is 0.4:1kg / kg, and then sterilize at 121°C under high temperature Bacteria, the sterilization time is 20min, and then placed to cool to room temperature, that is, prepared into a solid fermentation medium and set aside;

[0032] 3) Seed liquid preparation: After the Monascus test tube is activated, transfer it to a 90mm PDA plate medium for cultivation. The cultivation time is 4 days and the cultivation temperature is 32°C. , scraping the hyphae to obtain the Monascus suspension;

...

Embodiment 2

[0037] A preparation method rich in lovastatin functional red yeast rice, which comprises the steps of:

[0038] 1) Rice soaking: using indica rice as raw material, soak the indica rice in glacial acetic acid aqueous solution with a mass concentration of 0.2% for 4 hours, then pick up and drain, and collect for later use;

[0039] 2) High temperature sterilization: put 30g of indica rice treated in step (1) into a 250mL triangular flask, then add water, the weight ratio of the added water to rice is 0.4:1kg / kg, and then sterilize at 121°C under high temperature Bacteria, the sterilization time is 20min, and then placed to cool to room temperature, that is, prepared into a solid fermentation medium and set aside;

[0040]3) Seed liquid preparation: After the Monascus test tube is activated, transfer it to a 90mm PDA plate medium for cultivation. The cultivation time is 4 days and the cultivation temperature is 32°C. , scraping the hyphae to obtain the Monascus suspension;

[...

Embodiment 3

[0045] Detection of lovastatin in functional red yeast rice: Lovastatin standard product is Lovastatin (L0790000, sigma), according to the "People's Republic of China Light Industry Standards" QB / T 2487-2007 Annex A functional red yeast rice The content detection of lovastatin in the present invention is carried out by means of determination of Monacolin K content.

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Abstract

The invention discloses a preparation method of lovastatin-rich functional red yeast rice. The preparation method comprises the steps of rice soaking, high-temperature sterilization, seed solution preparation, inoculation, fermentation and drying. The preparation method is characterized in that in the fermentation step, the fermentation culture time is controlled at 18-24 d, cooling fermentation is adopted, the culture temperature is controlled at 30-35 DEG C on the 0-5th d during fermentation culture, and the culture temperature is controlled at 22-27 DEG C from the 6th d of fermentation culture to the end of fermentation; during fermentation culture, first yeast turning is performed on the 3d d of fermentation culture, and water replenishment and humidity control are performed by fermentation supernatant; second yeast turning is performed on the 5th d of fermentation culture, and water replenishment and humidity control are performed by fermentation supernatant; yeast turning is performed once every day from 5th d to 16th d of fermentation culture, and water replenishment and humidity control are performed; water replenishment and humidity control are performed by fermentation supernatant on the 12th d and 16th d, and water replenishment and humidity control are performed by sterile water for the rest of time; and yeast turning is performed once every two days after the 16thd of fermentation culture until the end of fermentation. The preparation method can increase the content of lovastatin in functional red yeast rice and enhance the fermentation effect of the functional red yeast rice and product quality.

Description

technical field [0001] The invention belongs to the technical field of microbial solid-state fermentation, and in particular relates to a preparation method of lovastatin-rich functional red yeast rice. Background technique [0002] Red yeast rice / powder, a medicinal and food product fermented from red yeast rice, is a traditional color symbol in my country with a history of more than one thousand years. The production of red yeast rice is mainly concentrated in Southeast Asian countries. It has the functions of regulating blood lipids, blood pressure, enhancing immunity, and increasing antibodies. It has been widely used in the food industry. The provinces that eat red yeast rice in my country are mainly concentrated in Jiangsu, Shanghai, Fujian, Jiangxi, Zhejiang, Shandong and other provinces and cities, and the per capita consumption of red yeast rice is 14-80g / d. Red yeast rice products on the market are mainly concentrated in "pigmented red yeast rice" (containing pigm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L7/104A23L33/00A61K36/899A61K36/062A61P3/06
CPCA23L7/104A23L33/00A61K36/899A61K36/062A61P3/06A61K2236/19
Inventor 刘俊阳晓代林亲录白婕
Owner 湖南微著生物科技有限公司
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