MicroRNA328 for regulating expression of TERT gene and application of microRNA328

Abstract

The present invention discloses a microRNA328 for regulating expression of TERT gene and an application of the microRNA328, and provides uses of the microRNA328 or a substance inhibiting expression ofthe microRNA328. The miR328 is found to have a significant inhibitory effect on an expression level of TERT mRNA in Hela cells and an oligonucleotide inhibitor of the miR-328 has a significant up-regulation effect on the expression level of the TERT gene mRNA in the Hela cells. The inhibitor of the miR-328 significantly increases angiogenic capacity of EPC. The EPC transfected by the inhibitor ofthe miR-328 significantly inhibits differentiation of NSCs into glial cells. Studies show that using miRNAs as targets or tools to enhance telomerase activity provide theoretical basis for treatmentof neurological diseases of vascular dementia (VD), etc. and have potential clinical application value.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to microRNA328 regulating TERT gene expression and application thereof. Background technique

[0002] Vascular dementia (VD) refers to the clinical syndrome of cerebral intelligence and cognitive dysfunction caused by brain dysfunction caused by cerebrovascular disease, mainly manifested as learning, memory, thinking and other obstacles. It is the second leading cause of dementia after silent disease (AD). With the acceleration of the aging process of the world population, the number of patients with VD has increased significantly. According to epidemiological statistics, the prevalence rate of the elderly over 65 years old is 1%-4%, and the prevalence rate of the elderly aged 85 is as high as 14%- 16%. Alzheimer's disease, including VD, has become the fourth leading cause of death in the elderly after tumors, heart disease, and stroke. Therefore, finding anti-VD drugs an...

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