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Preparation method and application of artificial exosome complex

A compound and exosome technology, applied in the field of biochemistry, can solve the problems of limited durability of skin care, large molecular weight of hyaluronic acid, ineffective deep skin cell absorption, etc., to achieve the effect of promoting absorption and controllable operation steps

Active Publication Date: 2020-01-03
SHANGHAI CHEERMORE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hyaluronic acid, also known as hyaluronic acid, is a natural moisturizing lubricant. Due to the large molecular weight of hyaluronic acid, it cannot be effectively absorbed by deep skin cells, and the durability of skin care is limited.

Method used

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  • Preparation method and application of artificial exosome complex
  • Preparation method and application of artificial exosome complex
  • Preparation method and application of artificial exosome complex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Detection of skin irritation by artificial exosome complex

[0037] Detection method:

[0038] Recruit 10 volunteers, use the newly prepared artificial exosomes with a concentration of 1mg / mL to evenly smear on the inner side of the left wrist, and apply ddH2O on the inner side of the right wrist as a control group, and let it stand for 20 minutes after application, and continue to observe the changes on the inner side of the wrist , and recorded whether the volunteers had irritating sensations such as pain and itching.

[0039] Experimental results such as figure 1 shown.

[0040] Table 1. Statistical results of artificial exosomes on skin test

[0041]

[0042] Experimental results:

[0043] The 10 volunteers did not observe redness and swelling after skin irritation within 20 minutes after applying the artificial exosomes, and none of the 10 volunteers felt irritating pain or itching.

Embodiment 2

[0044] Example 2. Cytotoxicity detection of artificial exosomes

[0045] Detection method:

[0046] Human immortalized epidermal cells Hacat were cultured, cells were digested and plated in 96-well plates, 5000 cells / well. Set the gradient concentration of artificial exosomes to incubate Hacat cells, continue to culture for 48 hours, observe the morphology of the cells during this period, and finally use the MTT method to detect the effect on cell proliferation.

[0047] Experimental results such as figure 2 , 3 shown.

[0048] Experimental results:

[0049] Artificial exosomes did not show obvious cytotoxicity at the cellular level, and had no effect on cell proliferation.

Embodiment 3

[0050] Example 3. Detection of the delivery efficiency of active substances by artificial exosomes

[0051] Detection method:

[0052] Using artificial exosomes as carriers, the activity of green fluorescent plasmid pCDNA3.1-eGFP delivered into cells was detected on Hacat cells.

[0053] Experimental results such as Figure 4 shown.

[0054] Experimental results:

[0055] Artificial exosomes have a good effect of coating active substances, and can effectively deliver active substances into cells.

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Abstract

The present invention belongs to the technical field of biochemistry and particularly relates to a preparation method and an application of an artificial exosome complex. Raw materials for preparing the complex are linear polyethyleneimines (PEI) and hyaluronic acid (HA). In preparation processes of HA-PEI, the linear polyethyleneimines can be obtained, while most polyethyleneimines are currentlybranched; an HA-PEI preparation scheme can quantitatively prepare the HA-PEI with a specific molecular weight; operation steps are controllable and the artificial exosome complex can be produced on alarge scale; and the HA-PEI can promote absorption of encapsulated active substances by cells in deep skin through ligand activity of the hyaluronic acid and membrane permeability of the linear polyethyleneimines.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a preparation method and application of an artificial exosome complex. Background technique [0002] The effectiveness of skin care products mainly lies in the absorption efficiency of active substances. Currently, there are few effective active substance delivery media on the market. Because linear polyacetylimide has both hydrophilic groups and lipophilic groups, it can form a bimolecular layer through the form of hydrophilic groups inside and outside lipophilic groups, and wrap substances to form microcapsules. Microcapsules It can use its own membrane fusion to fuse with the cell membrane and deliver inclusions into the cell. However, since the microcapsules utilize membrane fusion properties, they do not have cell selectivity. Hyaluronic acid, also known as hyaluronic acid, is a natural moisturizing lubricant. Due to the large molecular weight of hyaluroni...

Claims

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Application Information

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IPC IPC(8): A61K8/84A61K8/73A61Q19/00
CPCA61K8/84A61K8/735A61Q19/00A61K2800/592A61K8/14A61K2800/57A61K9/0014A61K9/5192A61K9/5107A61K9/1275A61K47/36A61K47/34A61K31/785A61K31/728C08G73/0206A61K2300/00
Inventor 李俊翔朱才彬
Owner SHANGHAI CHEERMORE BIOLOGICAL TECH CO LTD
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