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Ulvan composition with anti-tumor activity and preparation method thereof

A technology of anti-tumor activity and Ulva polysaccharide, which is applied in the field of medicine, can solve the problems that there is no anti-tumor activity of compound Ulva polysaccharide, and achieve the effect of small side effects

Inactive Publication Date: 2020-01-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the currently published patents related to seaweed polysaccharides, there is no research report on compound Ulva polysaccharides and its anti-tumor activity.

Method used

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  • Ulvan composition with anti-tumor activity and preparation method thereof
  • Ulvan composition with anti-tumor activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Soak Ulva in clear water, desalinate, dry and pulverize, then add 60% ethanol solution to the obtained Ulva dry powder in an amount of 20mL / g, reflux for extraction for 1 hour, and dry after reflux to obtain dried Degreased Ulva powder, recovered ethanol can be recycled;

[0038] 2) Add distilled water to the Ulva powder prepared in step 1) in an amount of 30mL / g, and extract with 350W ultrasonic wave at an extraction temperature of 60°C for 120 minutes, and then leaching in hot water at 80°C for 2 hours, After the extraction, filter, repeat the extraction of the filter residue twice according to the above conditions, combine the filtrate, centrifuge at 4000rpm for 10 minutes, and concentrate the supernatant under reduced pressure at 65°C to 1 / 10 of the original volume. Ethanol makes the volume concentration of ethanol in the solution reach 80%, then stand at 4°C for 8 hours, filter the precipitate, wash with absolute ethanol, acetone, ether in turn, and dry under re...

Embodiment 2

[0055] 1) Soak Ulva in clear water, desalinate, dry and pulverize, then add ethanol solution with a volume concentration of 70% to the obtained Ulva dry powder in an amount of 25mL / g, reflux for extraction for 2 hours, and dry after reflux to obtain dried Degreased Ulva powder, recovered ethanol can be recycled;

[0056] 2) Add distilled water to the Ulva powder prepared in step 1) in an amount of 35mL / g, and extract with 400W ultrasonic wave at an extraction temperature of 65°C for 120 minutes, and then leaching in hot water at 85°C for 3 hours, After the extraction is completed, filter, repeat the extraction of the filter residue 3 times according to the above conditions, combine the filtrate, centrifuge at 4500rpm for 15 minutes, and concentrate the supernatant under reduced pressure at 65°C to 1 / 10 of the original volume, then add Ethanol makes the volume concentration of ethanol in the solution reach 80%, then stand at 4°C for 12 hours, filter the precipitate, wash with a...

Embodiment 3

[0073] 1) Soak Ulva in clear water, desalinate, dry and pulverize, then add an ethanol solution with a volume concentration of 80% to the obtained Ulva dry powder in an amount of 30mL / g, reflux for extraction for 2 hours, and dry after reflux to obtain dried Degreased Ulva powder, recovered ethanol can be recycled;

[0074] 2) Add distilled water to the Ulva powder prepared in step 1) in an amount of 40mL / g, and extract with 400W ultrasonic wave at an extraction temperature of 70°C for 120 minutes, and then leaching in hot water at 90°C for 3 hours, After the extraction, filter, repeat the extraction of the filter residue 3 times according to the above conditions, combine the filtrate, centrifuge at 5000rpm for 20 minutes, and concentrate the supernatant under reduced pressure at 70°C to 1 / 10 of the original volume, and then add Ethanol makes the volume concentration of ethanol in the solution reach 80%, then stand at 4°C for 12 hours, filter the precipitate, wash with absolut...

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Abstract

The invention discloses an ulvan composition with anti-tumor activity and a preparation method thereof. The preparation method comprises the following steps: obtaining a crude extract from green algaulva lactuca by utilizing ultrasonic assisted extraction and separation of a water-extraction and alcohol-precipitation method, and performing further purification to obtain active ulvan; and mixing the active ulvan with the prepared ganoderma extract, gynostemma extract, red yeast rice extract, selenium-enriched yeast and tea polyphenol in a proportion. Animal pharmacodynamics prove that the ulvan composition has remarkable killing capability on H22 liver cancer tumors in a KM mouse body, can still achieve an excellent tumor inhibiting effect under the situation of insufficient immune, does not have remarkable side effect, and is a safe bioactive material formula with no toxicity and small side effect, so that a new way of ulva is provided to further utilization of marine drug resources,and a new choice is provided to medicines for treating or preventing tumors.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a compound ulva polysaccharide with auxiliary antitumor effect and a preparation method thereof. Background technique [0002] As a malignant disease, tumor has a high fatality rate, and patients are also troubled by its incidental diseases, and its prevalence is increasing year by year, and the society's demand for anti-tumor drugs is also increasing year by year. Traditionally used anti-tumor drugs often have various side effects or are very expensive. Therefore, there is a considerable demand for new anti-tumor drugs with high efficiency, low side effects, low cost and wide applicability in the market. [0003] Natural high molecular compounds in seaweed, such as polysaccharides, have certain anti-tumor effects, and have become the key direction for the development of new anti-tumor drugs. Among them, Ulva, also known as sea cabbage, Yantouqing, etc., is widely d...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61P35/00C08B37/00A61K31/353A61K31/715A61K33/04
CPCA61K31/353A61K31/715A61K33/04A61K36/06A61K36/062A61K36/074A61K36/424A61K36/82A61K36/899A61P35/00C08B37/0003A61K2300/00
Inventor 赵超吴德胜林国鹏刘丹
Owner FUJIAN AGRI & FORESTRY UNIV
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