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A method for extracting DHA and EPA in fish oil by combining supercritical fluid

A technology of supercritical fluid and fish oil, applied in chemical instruments and methods, preparation of organic compounds, separation/purification of carboxylic acid esters, etc., can solve degumming, decolorization, deodorization, cumbersome deacidification process, excessive solvent residue, Solve problems such as low separation purity, achieve the effect of reducing the amount of solvent used, avoiding solvent recovery, and reducing production costs

Active Publication Date: 2022-02-15
青岛利和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction and separation of DHA and EPA usually adopts urea synthesis method, silver nitrate complex method, lipase catalysis method, fish oil esterification method, vacuum distillation method, etc. Too many residues, low separation purity, and high temperature can easily lead to oxidative decomposition of substances. Therefore, how to provide a method for extracting DHA and EPA with less solvent consumption and high separation purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of method combining supercritical fluid to extract DHA and EPA in fish oil, comprises the steps:

[0036] S01. Degumming:

[0037] Weigh 1500g fish oil, and measure its acid value according to the hot ethanol method to be 6.83mgKOH / g; add phosphoric acid in an amount of 1.33% by weight of fish oil, heat to 80°C and continuously stir vigorously for 30min to flocculate the colloids in the fish oil Precipitated, and filtered to remove the colloid to obtain degummed fish oil (1329g).

[0038] S02. Decolorization and deodorization:

[0039] Carry out supercritical carbon dioxide extraction to degummed fish oil (1329g), extraction pressure 11MPa, extraction temperature 37 ℃, separation temperature 40 ℃, separation pressure 7MPa, carbon dioxide flow rate 40L / h, extraction time 3h, obtain decolorization and deodorization fish oil (1215.5g), measure Its acid value is 2.53 mgKOH / g.

[0040] S03. Deacidification:

[0041] Take by weighing S0 The decolorized and deodoriz...

Embodiment 2

[0054] A kind of method combining supercritical fluid to extract DHA and EPA in fish oil, comprises the steps:

[0055] S01. Degumming:

[0056] Weigh 1500g fish oil, and measure its acid value according to the hot ethanol method to be 6.83mgKOH / g; add phosphoric acid in an amount of 2% by weight of fish oil, heat to 60°C and continuously stir vigorously for 45min to flocculate the colloids in the fish oil Precipitated, and filtered to remove colloids to obtain degummed fish oil (1215g).

[0057] S02. Decolorization and deodorization:

[0058] Degummed fish oil (1215g) was subjected to supercritical carbon dioxide extraction at an extraction pressure of 15MPa, an extraction temperature of 40°C, a separation temperature of 50°C, a separation pressure of 8.5MPa, a carbon dioxide flow rate of 55L / h, and an extraction time of 3.5h to obtain decolorized and deodorized fish oil (972.8g) , and its acid value was determined to be 2.08mgKOH / g.

[0059] S03. Deacidification:

[0060...

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Abstract

The invention discloses a method for extracting DHA and EPA in fish oil combined with a supercritical fluid, which comprises the steps of degumming, decolorization and deodorization, deacidification, methylation reaction, low-temperature freezing separation, refining and glycerinization. The method for extracting DHA and EPA has the advantages of less solvent consumption, low production cost, and high separation purity of DHA and EPA.

Description

technical field [0001] The invention belongs to the technical field of supercritical extraction, and in particular relates to a method for extracting DHA and EPA in fish oil by combining supercritical fluid. Background technique [0002] Fish oil, derived from large marine fish, is a general term for all oily substances in fish, rich in DHA and EPA. Both DHA and EPA are essential fatty acids that cannot be synthesized by the human body, have important physiological activities, and have been widely used in health foods and medicines. DHA and EPA are chemically and thermally unstable substances, which are prone to polymerization, degradation, oxidation and other reactions, and the boiling point of high-purity DHA and EPA is quite high, making the conditions for separation and purification of high-purity DHA and EPA very harsh. [0003] At present, the extraction and separation of DHA and EPA usually adopts urea synthesis method, silver nitrate complex method, lipase catalysis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C67/60C07C67/58C07C67/52C07C67/54C07C67/48C07C69/587C07C51/41C07C57/03
CPCC07C67/60C07C67/58C07C67/52C07C67/54C07C67/48C07C51/412C07C69/587C07C57/03Y02P20/54
Inventor 张永昌赵楠张立华王斌孙西丽
Owner 青岛利和生物科技有限公司