A kind of dgt device and its application
A technology of filter membrane and diffusion phase, applied in the field of DGT, can solve the problem that the total amount of pollutants cannot truly and effectively reflect the biological effectiveness of pollutants, and achieve the effect of simple detection method and convenient use
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Embodiment 1
[0036] see figure 1 As shown, a DGT device includes a filter membrane 1, a diffusion phase 2, a binding phase 3 and a housing 4 for fixing the filter membrane 1, the diffusion phase 2 and the binding phase 3, and the filter membrane 1 It is a 0.45 μm polyvinylidene fluoride (PVDF) organic filter membrane, the diffusion phase 2 is a membrane made of agarose cross-linked acrylamide gel, and the binding phase 3 is a membrane made of divinylbenzene and N-ethylene The macroporous copolymer formed by polymerizing two monomers of pyrrolidone with a mass ratio of 1:1.2 was added to the agarose-crosslinked acrylamide solution to form a membrane.
[0037] The preparation method of described diffusion phase is as follows:
[0038] S101, preparing acrylamide with a mass concentration of 30%: accurately weigh 18.00 g of acrylamide and dissolve it in 60 mL of ultrapure water;
[0039] S102. Prepare an agarose cross-linking agent with a mass concentration of 2%. Take 2mL of 50×TAE and add ...
Embodiment 2
[0052] The difference with the foregoing embodiments is that the method for utilizing the DGT device of embodiment 1 to detect the free dissolved state concentration of oxytetracycline in the water body may further comprise the steps:
[0053] Step 1, prepare an antibiotic solution containing oxytetracycline, set the following concentration gradients: 0, 0.2, 0.5, 1.0, 2.5, 5.0 and 10.0mg / L, prepare 100mL for each concentration gradient, and put it in a constant temperature incubator Incubate at 25°C for 5 hours, and take samples to determine the concentration of oxytetracycline;
[0054] Step 2, add the solution prepared in step 1 into 50mL beakers in 3 parts, 30mL in each beaker. Put the pre-installed DGT device into the beaker, seal the mouth of the beaker with plastic wrap, put it in a constant temperature incubator at 25°C, and place it for 24 hours;
[0055] Step 3, take out the DGT device in step 2 from the beaker, rinse it 4 times with deionized water, remove the anti...
Embodiment 3
[0058] The difference with the foregoing embodiments is that the method for utilizing the DGT device of embodiment 1 to detect the free dissolved concentration of aureomycin in the water body may further comprise the steps:
[0059] Step 1, prepare an antibiotic solution containing chlortetracycline, set the following concentration gradients: 0, 0.2, 0.5, 1.0, 2.5, 5.0 and 10.0mg / L, prepare 100mL for each concentration gradient, and put it in a constant temperature incubator Incubate at 25°C for 5 hours, and take samples to determine the concentration of aureomycin;
[0060] Step 2, add the solution prepared in step 1 into 50mL beakers in 3 parts, 30mL in each beaker. Put the pre-installed DGT device into the beaker, seal the mouth of the beaker with plastic wrap, put it in a constant temperature incubator at 25°C, and place it for 24 hours;
[0061] Step 3, take the DGT device in step 2 out of the beaker, rinse it with deionized water 5 times to remove the antibiotic solutio...
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