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Method for determining content of nicarbazin in feed

A nicarbazine and feed technology, applied in the field of nicarbazine detection, can solve problems such as complex operation, low recovery rate, low repeatability, and cumbersome column passing steps, and achieve the goal of optimizing inspection steps, optimizing detection standards, and improving inspection efficiency Effect

Inactive Publication Date: 2020-01-10
贵州省兽药饲料监察所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for determining nicarbazine content in feed with high sensitivity, good accuracy and fast analysis speed, and to solve the cumbersome column passing steps in the prior art through reasonable optimization conditions , complex operation, low recovery rate, low repeatability, etc.

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  • Method for determining content of nicarbazin in feed
  • Method for determining content of nicarbazin in feed
  • Method for determining content of nicarbazin in feed

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0029] Embodiment 1: the assay method of nicarbazine content in the feed,

[0030] The methods for determining the content of nicarbazine in feed include the following:

[0031] 1. Pretreatment

[0032] Accurately weigh 10g of the sample (accurate to 0.01g) into a stoppered Erlenmeyer flask, add 50mL of dimethylformamide (DMF), shake with an electric oscillator for 30min, dry filter with filter paper, discard the initial filtrate, and collect the filtrate Pass through a 0.45 μm filter membrane and set aside.

[0033] 2. Analysis by liquid chromatography

[0034] The test sample solution is analyzed by a liquid chromatograph, and the nicarbazine peak area is determined.

[0035] 3. Calculate the content of nicarbazine in the feed

[0036] The peak area of ​​nicarbazine was measured by multiple groups of nicarbazine solutions with known mass concentration, and the mass concentration of nicarbazine was taken as the abscissa, and the corresponding peak area was taken as the or...

Embodiment 2

[0058] Embodiment 2: repeatability, accuracy, sensitivity verification

[0059] (1) Repeatability verification

[0060] Get a test sample solution, repeat sample injection 6 times, measure the nicarbazine peak area, and calculate the RSD (relative standard deviation) of the nicarbazine peak area to be 0.12%. It can be seen that the repeatability of the method of the present invention is better.

[0061] (2) Accuracy verification

[0062] Add nicarbazine variable standard solution in the blank sample of feed, make the test solution that addition amount is 12.5mg / kg, 25.0mg / kg, 50.0mg / kg, each addition amount prepares 3 parts in parallel, according to embodiment 1 The pretreatment method is to prepare the test solution, and measure it on the machine, and measure each sample once. The measurement results are shown in Table 3.

[0063] Table 3 nicarbazine recovery rate determination result

[0064]

[0065] It can be seen from Table 3 that the average recovery rate of samp...

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Abstract

The invention discloses a method for measuring the content of nicarbazin in feed, and the method comprises the following steps: (1) weighing a to-be-measured feed sample, adding dimethylformamide, shaking the mixture, performing an extracting operation, and performing filtering to obtain a test sample solution; (2) analyzing the test sample solution on a liquid chromatograph, and determining the peak area of nicarbazin; (3) determining the nicarbazin peak area by utilizing multiple groups of nicarbazin solutions with known mass concentrations, performing linear regression by taking the nicarbazin mass concentrations as horizontal coordinates and the corresponding peak areas as vertical coordinates, and establishing a nicarbazin linear regression equation, calculating the mass concentrationof nicarbazin in the feed to be detected by utilizing the nicarbazin linear regression equation according to the nicarbazin peak area of the feed sample to be detected, and then calculating the content of nicarbazin in the feed to be detected. By referring to the original detection standard, the detection steps are optimized, the detection efficiency is improved, and the recovery rate and repeatability can meet the requirements.

Description

technical field [0001] The invention discloses a method for determining the content of nicarbazine in feed, belonging to the technical field of nicarbazine detection. Background technique [0002] Nicarbazine is a complex of dinitrodiphenylurea and hydroxydimethylpyrimidine. It is yellow or yellow-green powder; odorless, slightly peculiar smell. Slightly soluble in dimethylformamide, insoluble in water, ethanol, ethyl acetate, chloroform, ether. The two components of nicarbazine can be absorbed by the poultry digestive tract and widely distributed in tissues and body fluids, and are used to prevent coccidiosis in chickens. "Specifications for the Use of Feed Drug Additives" has different regulations on the use of different types of nicarbazine premixes, which stipulates that the content of nicarbazine per 1kg of feed ranges from 20-125mg. Because nicarbazine can reduce egg production rate, fertilization rate, and egg quality and lighten the color of brown eggshells, it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 孙播东章厉劼钱莘莘罗永成周艺林郭萍周泽晓廖欢杨强赵贵焦仁刚古海王培峰谢丽丽
Owner 贵州省兽药饲料监察所