Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

JE vaccine soluble microneedle patch and preparation method thereof

A soluble, micro-needle sticking technology, applied in biochemical equipment and methods, microorganisms, pharmaceutical formulations, etc., can solve the problems of decreased mechanical strength of soluble micro-needles, and achieve the best immune enhancement effect, high mechanical strength of the needle body, immune good effect

Pending Publication Date: 2020-01-14
LIAONING CHENGDA BIOTECH
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to references (Park J H, Allen M G, Prausnitz MR. Polymer microneedles for controlled-release drug delivery [J]. Pharmaceutical research, 2006, 23(5): 1008-1019.), it can be seen that as drugs account for As the ratio increases, the mechanical strength of the prepared soluble microneedles decreases accordingly.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • JE vaccine soluble microneedle patch and preparation method thereof
  • JE vaccine soluble microneedle patch and preparation method thereof
  • JE vaccine soluble microneedle patch and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The negative mold preparation of embodiment 1 Japanese encephalitis vaccine microneedles

[0033] Such as figure 1 , the present invention adopts mold-inverting method to prepare the female mold of Japanese encephalitis vaccine microneedle, and the steps are as follows:

[0034] Place the metal male mold microneedle in the cuboid container with the needle tip facing upward. The polydimethoxysiloxane (PDMS) and the curing agent are configured according to the mass ratio of 10:1 and then poured into a rectangular parallelepiped container; the container is placed in a vacuum drying oven, and the vacuum degree of the parameters is respectively set to 0.07MPa, The time is 5 minutes to remove the air bubbles in the mixed solution; then put the container into an oven, set the temperature parameter to 50°C, take it out after 5-8 hours, and demould to obtain a PDMS microneedle female mold.

Embodiment 2

[0035] The preparation of embodiment 2 JE vaccine microneedles

[0036] Such as figure 2 , the present invention adopts secondary centrifugation into mold method to prepare Japanese encephalitis vaccine microneedle, and the steps are as follows:

[0037] 1) After mixing the various ingredients in the prescription in proportion, dissolve it with an appropriate amount of solvent (deionized water) to form a uniform mixture (i.e. needle body fluid), take an appropriate amount of needle body fluid into a centrifuge tube, place it in a centrifugal precipitator, and centrifuge Remove the air bubbles in the needle body fluid and let it stand for later use.

[0038] 2) Take the needle body fluid obtained after centrifugation in step 1), pour it on the PDMS mold prepared in Embodiment 1 of the present invention, then place the PDMS mold in a desktop low-speed centrifuge, and centrifuge at a speed of 3000 rpm for 5 minutes, so that the needle body fluid is injected into the hole of the...

Embodiment 3

[0041] Example 3 Effect of the use of different immune adjuvants on the immune effect of Japanese encephalitis vaccine soluble microneedles

[0042] Assuming that the proportion of the immune adjuvant in the total mass of the needle is a certain value, only the components of the immune adjuvant are changed, and the influence of different combinations of the immune adjuvant as shown in Table 1 on the immune effect of the microneedle is investigated. This example For the related microneedle and negative mold, its preparation method refers to Example 1 and Example 2 of the present invention.

[0043] Table 1 Effect of different immune adjuvants on the immune effect of JE vaccine soluble microneedles

[0044]

[0045]

[0046] For 11 microneedle prescriptions in Table 1, after making soluble microneedles with reference to the method of embodiment 1 and embodiment 2 of the present invention, detect the immune effect of soluble microneedles as follows:

[0047] Eighteen solub...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a JE (Japanese encephalitis) vaccine soluble microneedle patch, which comprises a needle body and a backing. The needle body is composed of a JE vaccine, a matrix material and an adjuvant, wherein the adjuvant is a mixture of MDP and GM-CSF; and the content ratio of the adjuvant to the Japanese encephalitis vaccine is (1: 1) to (1: 3). The JE vaccine soluble microneedle prepared in the invention has good immune effect and high mechanical strength of the needle body, and is expected to replace a JE vaccine injection and realize painless minimally invasive administration.

Description

technical field [0001] The invention relates to the technical field of vaccine microneedle administration, and provides a JE vaccine soluble microneedle patch and a preparation method thereof. Background technique [0002] Japanese encephalitis is an acute infectious disease caused by the Japanese encephalitis virus that invades the central nervous system, often causing death or neurological sequelae. Japanese encephalitis vaccine is an effective measure to prevent epidemic Japanese encephalitis. The current JE vaccine is generally administered through needle injection, which has poor patient compliance. Therefore, a new route of vaccine administration is urgently needed. In addition, protein drugs are easily decomposed by proteases in the gastrointestinal tract or the first-pass effect of the liver after oral administration, resulting in a decrease in activity, thereby affecting their bioavailability. Transdermal drug delivery system (TDDS) is a popular new technology for ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/12A61K39/39A61K9/00A61K47/36A61P31/14
CPCA61K39/12A61K39/39A61K9/0021A61K47/36A61P31/14C12N2770/36034A61K2039/55522A61K2039/5555Y02A50/30
Inventor 张庶民马凤森周荔葆廖辉陈中秋刘苗苗吴铮辛小韵
Owner LIAONING CHENGDA BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com