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Biochar-based aflatoxin prevention and control slow release fertilizer and preparation method thereof

A technology of aflatoxin and biochar, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as environmental threats, damage to soil physical and chemical properties, and decline in soil fertility, so as to reduce infestation and enhance Immunity, effect of increasing soil organic matter content

Pending Publication Date: 2020-01-14
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Long-term application of common chemical fertilizers will seriously damage the physical and chemical properties of the soil, resulting in a significant decline in soil fertility and a certain threat to the environment

Method used

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  • Biochar-based aflatoxin prevention and control slow release fertilizer and preparation method thereof
  • Biochar-based aflatoxin prevention and control slow release fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The waste corn stalks were air-dried in the natural state and ground to 3mm, carbonized in a tube-type high-temperature sintering furnace at 500°C with a heating rate of 10°C / min for 2-3 hours to obtain biochar, and the obtained biochar was passed through 100 Mesh sieve;

[0056] Heat the modified starch and sodium alginate with a mass ratio of 3:1 to about 50°C with a magnetic stirrer and stir for 1 hour to prepare a composite starch binder with a concentration of 6%.

[0057] Inoculate the non-toxin-producing Aspergillus flavus on the solid medium, and culture at a temperature of 28°C to 32°C for 3 to 5 days to obtain activated bacteria;

[0058] Gained activated bacteria are inoculated into the seed culture medium and shaken for 8 to 24 hours to obtain non-toxic Aspergillus flavus bacteria liquid;

[0059] Picking Bacillus subtilis and inoculating it on a liquid medium for cultivation to obtain a Bacillus subtilis bacteria liquid;

[0060] The biochar of 60 parts b...

Embodiment 2

[0062] The waste corn stalks were air-dried in the natural state and ground to 3mm, carbonized in a tube-type high-temperature sintering furnace at 500°C with a heating rate of 10°C / min for 2-3 hours to obtain biochar, and the obtained biochar was passed through 100 Mesh sieve;

[0063] Heat the modified starch and sodium alginate with a mass ratio of 3:1 to about 50°C with a magnetic stirrer and stir for 1 hour to prepare a composite starch binder with a concentration of 6%.

[0064] Inoculate the non-toxin-producing Aspergillus flavus on the solid medium, and culture at a temperature of 28°C to 32°C for 3 to 5 days to obtain activated bacteria;

[0065] Gained activated bacteria are inoculated into the seed culture medium and shaken for 8 to 24 hours to obtain non-toxic Aspergillus flavus bacteria liquid;

[0066] Picking Bacillus subtilis and inoculating it on a liquid medium for cultivation to obtain a Bacillus subtilis bacteria liquid;

[0067] The biochar of 65 parts b...

Embodiment 3

[0069] The waste corn stalks were air-dried in the natural state and ground to 3mm, carbonized in a tube-type high-temperature sintering furnace at 500°C with a heating rate of 10°C / min for 2-3 hours to obtain biochar, and the obtained biochar was passed through 100 Mesh sieve;

[0070] Heat the modified starch and sodium alginate with a mass ratio of 3:1 to about 50°C with a magnetic stirrer and stir for 1 hour to prepare a composite starch binder with a concentration of 6%.

[0071] Inoculate the non-toxin-producing Aspergillus flavus on the solid medium, and culture at a temperature of 28°C to 32°C for 3 to 5 days to obtain activated bacteria;

[0072] Gained activated bacteria are inoculated into the seed culture medium and shaken for 8 to 24 hours to obtain non-toxic Aspergillus flavus bacteria liquid;

[0073] Picking Bacillus subtilis and inoculating it on a liquid medium for cultivation to obtain a Bacillus subtilis bacteria liquid;

[0074] The biochar of 70 parts b...

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Abstract

The invention discloses a biochar-based aflatoxin prevention and control slow release fertilizer, which comprises, by weight, 60-70 parts of biochar, 10-15 parts of a nitrogen-phosphorus-potassium compound fertilizer, 8-10 parts of a compound starch binder, 4-6 parts of a non-toxic Aspergillus flavus liquid, 2-4 parts of a bacillus subtilis liquid, 1-3 parts of acetic acid and 1-2 parts of hydroxymethyl chitosan. According to the invention, biochar increases the content of the organic matters in soil and maintains the activity of the bacterial agent, the non-toxic strain inhibits the growth oftoxic strains so as to reduce the production of aflatoxins, and bacillus subtilis and chitosan can enhance the immunity of plants. The invention further provides a preparation method of the biochar-based aflatoxin prevention and control slow release fertilizer, wherein the concentration of the composite starch binder is controlled, and the activity and the effect of the biochar-based aflatoxin prevention and control slow release fertilizer are improved.

Description

technical field [0001] The invention relates to the field of biological control and planting of aflatoxins in crops, in particular to a biochar-based slow-release fertilizer for the prevention and control of aflatoxins and a preparation method thereof. Background technique [0002] Long-term application of common chemical fertilizers will seriously damage the physical and chemical properties of the soil, resulting in a significant decline in soil fertility and a certain threat to the environment. Biochar is a high-temperature treatment of biomass under anaerobic conditions. The oil and gas in the biomass are burned, and the rest is biochar. Biochar is rich in micropores. This pore structure is very easy to gather nutrients and beneficial microorganisms. Therefore, biochar can not only supplement the organic matter content of the soil, but also effectively preserve water and nutrients, thereby making the soil fertile and conducive to plant growth. , to increase production wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/60C05G3/40C12N1/14C12N1/20C12R1/67C12R1/125
CPCC05B1/02C05G3/00C12N1/14C12N1/20C05C9/00C05D1/02C05D9/00C05F11/08
Inventor 吴文福冯家畅窦建鹏孙长坡徐岩张由真尹冬雪李子林吴子丹
Owner JILIN UNIV