Method for pretreating caragana microphylla with ethylene diamine
A pretreatment, ethylenediamine technology, applied in fermentation and other directions, can solve problems such as strong biological resistance
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Embodiment example 1
[0014] Implementation Case 1: The method of ethylenediamine pretreatment Caragana and enzymatic hydrolysis of the present invention
[0015] (1) Pretreatment method
[0016] Mechanically crush the dried caragana raw material through a 40-mesh sieve, weigh 20 g of the obtained caragana material and put it into a 250 mL stainless steel reaction kettle with a sealed cover, add 30 mL of ethylenediamine, stir evenly, and tighten the sealed cover. Put the reaction kettle containing the solid-liquid mixture into an oil bath at 120°C for 10 minutes to react. After taking out the reaction kettle, put it into cold water to cool rapidly, open the reaction kettle, take out the material and wash it with deionized water (or recover ethylenediamine by heating), filter and dry to obtain the pretreated caragana bar.
[0017] (2) Enzymatic hydrolysis method
[0018] Take a certain amount of material pretreated by ethylenediamine and put it into a 100mL narrow-necked Erlenmeyer flask, add cell...
Embodiment example 2
[0019] Implementation Case 2: The method of ethylenediamine pretreatment Caragana and enzymatic hydrolysis of the present invention
[0020] (1) Pretreatment method
[0021] Mechanically crush the dried caragana raw material through a 60-mesh sieve, weigh 20 g of the obtained caragana material and put it into a 250 mL stainless steel reaction kettle with a sealed cover, add 70 mL of ethylenediamine, stir evenly, and tighten the sealed cover. Put the above-mentioned reactor containing the solid-liquid mixture into an oil bath at 180° C. to react for 30 minutes. After taking out the reaction kettle, put it into cold water to cool rapidly, open the reaction kettle, take out the material and wash it with deionized water (or recover ethylenediamine by heating), filter and dry to obtain the pretreated caragana bar.
[0022] (2) Enzymatic hydrolysis method
[0023] Take a certain amount of material pretreated by ethylenediamine and put it into a 100ml narrow-mouth Erlenmeyer flask,...
Embodiment example 3
[0024] Implementation Case 3: The method of ethylenediamine pretreatment of caragana and enzymatic hydrolysis of the present invention
[0025] (1) Pretreatment method
[0026] Mechanically pulverize the dried caragana raw material through an 80-mesh sieve, weigh 20 g of the obtained caragana material and put it into a 250 mL stainless steel reaction kettle with a sealed cover, add 100 mL of ethylenediamine, stir evenly, and tighten the sealed cover. Put the reaction kettle containing the solid-liquid mixture into an oil bath at 230°C for 60 minutes to react. After taking out the reaction kettle, put it into cold water to cool rapidly, open the reaction kettle, take out the material and wash it with deionized water (or recover ethylenediamine by heating), filter and dry to obtain the pretreated caragana bar.
[0027] (2) Enzymatic hydrolysis method
[0028] Take a certain amount of material pretreated by ethylenediamine and put it into a 100mL narrow-necked Erlenmeyer flask,...
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