Device and method for detecting nucleic acid by isothermal amplification technology

A nucleic acid and storage chamber technology, applied in the field of nucleic acid detection devices using constant temperature amplification technology, can solve the problems of harsh reagent storage conditions, easy attenuation, unstable fluorescent signals, etc. stable output

Inactive Publication Date: 2020-01-17
TDK CORPARATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, no matter for the existing PCR or isothermal amplification technology, fluorescence detection technology is still the main detection technology, and the fluorescence signal is unstable and easy to attenuate, and its reagent storage conditions are relatively harsh

Method used

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  • Device and method for detecting nucleic acid by isothermal amplification technology
  • Device and method for detecting nucleic acid by isothermal amplification technology

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Experimental program
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Effect test

Embodiment 1

[0038] A nucleic acid detection device according to an embodiment of the present invention includes a sample processor and a magnetic sensitive detector; the structure of the sample processor is as follows: figure 1 As shown, it includes a microfluidic channel 1, a thermostat 2, a capture chip storage chamber 301, a DNA modified magnetic bead storage chamber 302 and a cleaning solution storage chamber 303;

[0039] A reagent inlet and a reagent outlet are provided on the micro-flow channel 1; a temperature controller 2 is provided on the micro-flow channel 1;

[0040] The inlet of the capture chip storage chamber 301 communicates with the reagent outlet of the microfluidic cell 1 through the first microchannel 701, communicates with the DNA modification magnetic bead storage chamber 302 through the second microchannel 702, and stores it with the cleaning solution through the third microchannel 703. The chamber 303 communicates; the first microchannel 701 is provided with a fir...

Embodiment 2

[0061] A device for detecting nucleic acid according to an embodiment of the present invention differs from Embodiment 1 only in that the sample processor is different. The sample processor of the device for detecting nucleic acid in this embodiment is as follows: figure 2 As shown, the difference between it and the sample processor of Example 1 is: the sample processor of the device for detecting nucleic acid in this embodiment does not include a DNA extraction chamber 9 with a built-in DNA extraction solution, but the device for detecting nucleic acid in this embodiment The sample processor also includes an RNA extraction chamber 10 and a reverse transcription reagent storage chamber 11 with a built-in RNA extraction solution, and the reverse transcription reagent storage chamber 11 communicates with the reagent outlet of the RNA extraction chamber 10 and the reagent inlet of the DNA holding chamber 4 to be tested respectively; And in this embodiment, the pressurizer 8 is co...

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Abstract

The invention relates to a device and method for detecting nucleic acid by an isothermal amplification technology. The device comprises a sample processor and a magnet-sensitive detector, wherein thesample processor comprises a microflow groove, a temperature controller, a capture chip storage chamber, a DNA modification magnetic bead storage chamber and a cleaning fluid storage chamber; the temperature controller is arranged at the microflow groove; an inlet of the capture chip storage chamber communicates with a reagent outlet of the microflow groove through a first microchannel, communicates with the DNA modification magnetic bead storage chamber through a second microchannel and communicates with the cleaning fluid storage chamber through a third microchannel; valves are arranged on the first microchannel, the second microchannel and the third microchannel; and the magnet-sensitive detector comprises a magnetic sensor and a groove capable of accommodating the capture chip storagechamber, the capture chip storage chamber is inserted in the groove, and the magnetic sensor senses DNA modification magnetic beads in the capture chip storage chamber and converts magnetic signals ofDNA modification magnetic beads into electric signals. The device of the invention is high in response speed and stable in signal output when used for detecting the nucleic acid.

Description

technical field [0001] The invention relates to a device and method for detecting nucleic acid, in particular to a device and method for detecting nucleic acid using constant temperature amplification technology. Background technique [0002] Utilizing ordinary PCR (polymerase chain reaction) amplification and fluorescence detection is an existing common method for DNA detection. Conventional PCR machines currently in use have as many as dozens of PCR reaction tanks that can be precisely controlled, which allows the PCR machine to perform PCR reactions with different reaction conditions simultaneously and in large quantities. However, it is time-consuming and expensive for quick and simple DNA testing. Therefore, isothermal amplification technology (isothermal amplification) has been applied more and more. Isothermal amplification technology is to amplify nucleic acid in a short time under isothermal conditions. Compared with conventional PCR technology, it does not requir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6844
CPCC12Q1/6844C12Q1/6825B01L3/502738B01L3/502761B01L7/52B01L2200/025B01L2200/0621B01L2200/0631B01L2200/0668B01L2300/1805B01L2300/0636B01L2200/147B01L2400/0487B01L2400/043B01L2400/0666B01L2400/0638G01N27/745C12Q2563/143C12Q2565/501C12Q2521/30C12Q2527/101C12Q2565/607C12Q2565/629B01L3/502715C12Q1/686
Inventor 周飞冯先文万重军付瑜
Owner TDK CORPARATION
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