Kit for detecting 1, 5-AG with good stability and method

A detection kit, 5-AG technology, applied in 1 field, can solve the problems of weakened ability to eliminate glucose, poor stability of HK enzyme, poor stability of 1,5-AG detection kit, etc., to achieve good stability, The effect of high accuracy and precision and strong anti-glucose interference ability

Inactive Publication Date: 2020-01-17
CO HEALTH BEIJING LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor stability of the HK enzyme, the stability of the 1,5-AG detection kit is not good
And as the storage time prolongs, its ability to eliminate glucose weakens

Method used

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  • Kit for detecting 1, 5-AG with good stability and method
  • Kit for detecting 1, 5-AG with good stability and method
  • Kit for detecting 1, 5-AG with good stability and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation process of 1,5-AG detection kit in serum:

[0031] Composition and concentration of reagent R1:

[0032] MES (pH6.0) 50mmol / L

[0033] Hexokinase 15KU / L

[0034] Pyruvate kinase 10KU / L

[0035] ATP 5mmol / L

[0036] 4-Aminoantipyridine 3mmol / L

[0037] Phosphoenolpyruvate 3mmol / L

[0038] Triton X-100 5g / L

[0039] WD-001 5g / L

[0040] Magnesium chloride 6mmol / L

[0041] Proclin300 5g / L;

[0042] Composition and concentration of reagent R1:

[0043] HEPES (pH7.5) 50mmol / L

[0044] Pyranose oxidase 50KU / L

[0045] Peroxidase 20KU / L

[0046] p-Hydroxybenzoic acid 10mmol / L

[0047] Proclin300 5g / L

[0048] Triton X-100 5g / L.

[0049] Application of 1,5-AG detection kit in serum on automatic biochemical analyzer:

[0050] Analysis method: two-point endpoint method

[0051] Reaction Direction: Rising Reaction

[0052] Calibration method: line

[0053] Determination of dominant wavelength: 546nm

[0054] Determination of sub-wavelength: 700nm

[...

Embodiment 2

[0060] 1,5-AG assay kit performance evaluation

[0061] 1. Analytical sensitivity

[0062] The kit B of the present invention prepared in Example 1 and the commercially available 1,5-AG assay kit A were calibrated simultaneously with their respective calibrators, and the results are shown in Table 1.

[0063] Table 1 1,5-AG assay kits on sale and calibration results of the kits of the present invention

[0064]

[0065] As can be seen from Table 1, when the 1,5-AG content of the kit B of the present invention is 150 μmol / L, the absorbance change rate is 0.0572, and when the 1,5-AG content of the kit A on sale is 150 μmol / L, the absorbance The rate of change is 0.0330, which shows that the analytical sensitivity of the kit B of the present invention is significantly higher than that of the commercially available kit A.

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Abstract

The invention discloses a kit for detecting 1, 5-AG with good stability and a method in the field of medical detection. According to the kit disclosed by the invention, a reagent R1 and a reagent R2 are kept stable within an expiry date under the interference of high glucose concentration by selecting a proper method and a proper stabilizer. According to the kit, the content of 1, 5-AG in a humanserum sample is determined by adopting a pyranose oxidase method. The test result shows that the kit for detecting 1, 5-AG is high in glucose interference resistance compared with other kits sold on the market, can eliminate 1-30 mM/L of endogenous glucose interference, can be stored for 7 days stably under the 37 DEG C acceleration condition, has better acceleration stability than other 1,5-AG measurement kits on the market, has the advantages of high accuracy, high precision, wide linear range, good stability and the like, and can be widely used in the diagnosis and treatment of diabetes.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents and biochemical reagents, in particular to a stable 1,5-AG assay kit and a detection method thereof. Background technique [0002] The main goal of treating diabetes is to prevent the occurrence of its chronic complications, delay the progress of its chronic complications, reduce the disability rate and mortality rate and improve the quality of life of patients. The study found that serum I 5-AG can accurately detect slight changes in blood sugar, while fructosamine and HbA1c cannot. 1,5-AG reflects the changes in blood sugar in diabetic patients in the past week, the level of glycosylated hemoglobin mainly reflects the changes in blood sugar in the past 2-3 months, and the level of fructosamine reflects the changes in blood sugar in the past 10-14 days. Although this change can be found by measuring FPG, it is not as sensitive as 1,5-AG. The half-life of 1,5-AG is shorter w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N21/01
CPCG01N21/78G01N21/3103G01N21/01
Inventor 孟琛靳晓黎孙小芳
Owner CO HEALTH BEIJING LAB
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