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Preparation method and application of a dual-ratio aptasensor based on electrochemistry and photoelectrochemistry

An aptamer sensor, photoelectrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve the effects of good stability, improved selectivity, and high precision

Active Publication Date: 2022-02-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combining the dual detection method with the ratiometric method to construct a sensor for detection has not been reported

Method used

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  • Preparation method and application of a dual-ratio aptasensor based on electrochemistry and photoelectrochemistry
  • Preparation method and application of a dual-ratio aptasensor based on electrochemistry and photoelectrochemistry
  • Preparation method and application of a dual-ratio aptasensor based on electrochemistry and photoelectrochemistry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] according to figure 1 Described preparation process:

[0041] (1) Preparation of Au NPs

[0042] First, prepare 0.1mol·L -1 Chlorauric acid solution and 100mg·mL -1 Trisodium Citrate Solution. Take 200μL 0.1mol L -1 Chlorauric acid solution and 25mL ultrapure water were placed in a three-necked flask, heated in an oil bath until it boiled completely. Then add 0.25mL 100mg·mL -1 The trisodium citrate solution was continued to react for 15 minutes, cooled to room temperature naturally, and kept in a refrigerator at 4°C away from light for later use.

[0043] (2) Pretreatment of indium tin oxide glass electrode: Boil the indium tin oxide glass electrode in 1mol / L NaOH solution for 10 minutes, and then dry it in air after ultrasonication in absolute ethanol and ultrapure water. The diameter of the glass electrode is 6 mm (d = 6 mm ITO).

[0044] (3) The design of the bases of each nucleotide chain in the hybridization chain reaction, the 5' end of sDNA is marked wit...

Embodiment 2

[0058] (1) Preparation of Au NPs

[0059] First, prepare 0.1mol·L -1 Chlorauric acid solution and 100mg·mL -1 Trisodium Citrate Solution. Take 200μL 0.1mol L -1 Chlorauric acid solution and 25mL ultrapure water were placed in a three-necked flask, heated in an oil bath until it boiled completely. Then add 0.25mL 100mg·mL -1 The trisodium citrate solution was continued to react for 15 minutes, cooled to room temperature naturally, and kept in a refrigerator at 4°C away from light for later use.

[0060] (2) Pretreatment of indium tin oxide glass electrode: Boil the indium tin oxide glass electrode in 1mol / L NaOH solution for 15 minutes, and then dry it in air after ultrasonication in absolute ethanol and ultrapure water. The diameter of the glass electrode is 6 mm (d = 6 mm ITO).

[0061] (3) The design of the bases of each nucleotide chain in the hybridization chain reaction, the 5' end of sDNA is marked with thiol (-SH) to combine with AuNPs to achieve the immobilizatio...

Embodiment 3

[0075] (1) Preparation of Au NPs

[0076] First, prepare 0.1mol·L -1 Chlorauric acid solution and 100mg·mL -1 Trisodium Citrate Solution. Take 200μL 0.1mol L -1 Chlorauric acid solution and 25mL ultrapure water were placed in a three-necked flask, heated in an oil bath until it boiled completely. Then add 0.25mL 100mg·mL -1 The trisodium citrate solution was continued to react for 15 minutes, cooled to room temperature naturally, and kept in a refrigerator at 4°C away from light for later use.

[0077] (2) Pretreatment of indium tin oxide glass electrode: Boil the indium tin oxide glass electrode in 1mol / L NaOH solution for 20 minutes, and then dry it in air after ultrasonication in absolute ethanol and ultrapure water. The diameter of the glass electrode is 6 mm (d = 6 mm ITO).

[0078] (3) The design of the bases of each nucleotide chain in the hybridization chain reaction, the 5' end of sDNA is marked with thiol (-SH) to combine with AuNPs to achieve the immobilizatio...

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Abstract

The invention belongs to the technical field of biosensing and detection, and relates to a preparation method and application of a double-ratio aptamer sensor based on electrochemistry and photoelectrochemistry. Specifically, a novel dual-ratio aptasensor based on electrochemical and photoelectrochemical methods for the specific detection of streptomycin (STR) was proposed for the first time. The steps are as follows: step 1, preparation of gold nanoparticles (Au NPs) and design of each nucleotide chain base in the hybridization chain reaction; step 2, construction of aptasensor based on electrochemical and photoelectrochemical dual ratios. The double-ratio aptamer sensor based on electrochemical and photoelectrochemical methods constructed by the invention provides an effective way for reducing the influence of the external environment and accurate and wide-range detection of streptomycin.

Description

technical field [0001] The invention belongs to the technical field of biosensing and detection, and relates to a preparation method and application based on an electrochemical (EC) and photoelectrochemical (PEC) dual-ratio sensor, in particular to a sensor based on ferrocene (Fc) and methylene blue (MB). The preparation method and application of the streptomycin dual ratio sensor of the system. Background technique [0002] Streptomycin (STR) is an aminoglycoside antibiotic. Due to its good bactericidal effect and low price, the abuse problems such as frequent drug use and excessive drug use have become increasingly prominent, resulting in drug residues in crops, water bodies and soil. Long-term intake of fruits, vegetables, and agricultural products containing streptomycin residues will directly or indirectly threaten human and animal health, and studies have also shown that agricultural streptomycin has potential teratogenic effects. Therefore, the detection of streptomy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/3276G01N27/3278G01N27/305
Inventor 由天艳申秀丽刘东朱成喜李玉叶
Owner JIANGSU UNIV