Production system for preparing phycocyanin through low-temperature alcohol extraction as well as process method

A technology for phycocyanin and production system, which is applied in the field of production system for preparing phycocyanin by low-temperature alcohol extraction, can solve the problems of difficult control of production process and poor stability of phycocyanin, and achieves the effect of inhibiting the growth of microorganisms

Active Publication Date: 2020-01-21
WUHAN GREEN FOOD BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that phycocyanin has poor stability and its finished product has strict requirements on microbial content

Method used

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  • Production system for preparing phycocyanin through low-temperature alcohol extraction as well as process method
  • Production system for preparing phycocyanin through low-temperature alcohol extraction as well as process method
  • Production system for preparing phycocyanin through low-temperature alcohol extraction as well as process method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] see figure 1 , figure 2 , a production system for preparing phycocyanin by low-temperature alcohol extraction in the present embodiment has an extraction tank 1, and after the extraction tank 1, a first-stage cold cycle device 4 and a cold cycle centrifuge are sequentially connected in series through a pipeline 2 and a valve 3 5. Two-stage cold circulation device 6, one-stage microfiltration membrane filter 7, three-stage cold circulation device 8, two-stage ultrafiltration membrane filter 9, three-stage concentration membrane filter 10, spray drying tower 11, the two Both the first-stage ultrafiltration membrane filter 9 and the third-stage concentration membrane filter 10 are located in a 10°C constant-temperature aseptic storehouse 12, and the spray drying tower 11 is located in a room-temperature aseptic storehouse 13.

[0060] In the present invention, there are 1 to 7 extraction tanks 1, and in this embodiment, there are 5 extraction tanks, which are connected i...

Embodiment 2

[0075] A production system for preparing phycocyanin by low-temperature alcohol extraction of the present invention has an extraction tank, and after the extraction tank, a primary cold cycle device, a cold cycle centrifuge, a secondary cold cycle device, One-stage microfiltration membrane filter, three-stage cold cycle device, two-stage ultrafiltration membrane filter, three-stage concentration membrane filter, spray drying tower, the two-stage ultrafiltration membrane filter and three-stage concentration membrane filter are all It is located in a 10°C constant temperature aseptic warehouse, and the spray drying tower is located in a normal temperature aseptic warehouse.

[0076] In the present invention, there are 1 to 7 extraction tanks, and in this embodiment, there are 7 extraction tanks, which are connected in parallel, and the batching time and timing of each extraction tank are adjusted according to the process requirements during use. During the discharge time, the ex...

Embodiment 3

[0090] A production system for preparing phycocyanin by low-temperature alcohol extraction in this embodiment has an extraction tank, and after the extraction tank, a primary cold cycle device, a cold cycle centrifuge, and a secondary cold cycle device are sequentially connected in series through pipelines and valves , one-stage microfiltration membrane filter, three-stage cold cycle device, two-stage ultrafiltration membrane filter, three-stage concentration membrane filter, spray drying tower, the two-stage ultrafiltration membrane filter and three-stage concentration membrane filter They are all located in a 10°C constant temperature aseptic warehouse, and the spray drying tower is located in a normal temperature aseptic warehouse.

[0091] In the present invention, there are 1 to 7 extraction tanks, and in this embodiment, one extraction tank is provided specifically, and the extraction tank is provided with a jacket and a stirring device.

[0092] The first-stage cold cyc...

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Abstract

The invention discloses a production system for preparing phycocyanin through low-temperature alcohol extraction as well as a process method. The production system provided by the invention comprisesan extraction tank; the extraction tank is connected in series with a first-stage cold circulation device, a cold circulation centrifugal machine, a second-stage cold circulation device, a first-stagemicro-filtration membrane filtering machine, a third-stage cold circulation device, a second-stage ultrafiltration membrane filtering machine, a third-stage concentration membrane filtering machine and a spray-drying tower sequentially through pipelines and valves; and the second-stage ultrafiltration membrane filtering machine and the third-stage concentration membrane filtering machine are positioned in a constant-temperature (10 DEG C) sterile library. According to the method provided by the invention, spirulina powder is extracted by a low-content alcohol-containing phosphate buffer solution, low-concentration ethanol helps to break walls of spirulina cells, the total number of microorganisms in material liquid is controlled, low-temperature separation and purification are conducted by controlling the temperature to be below 10 DEG C in the whole process after extraction, and the total number of microbial colonies in the phycocyanin can be controlled to be within 100 cfu/g under the condition of not influencing the yield of the phycocyanin, so the production process of the phycocyanin is greatly optimized and the quality of products is improved.

Description

technical field [0001] The invention belongs to a method for preparing natural pigments, and in particular relates to a production system and a process method for preparing phycocyanin by low-temperature alcohol extraction. Background technique [0002] Phycocyanin is a natural blue pigment isolated from spirulina. It is not only a protein, but also an excellent natural food coloring, and it is also a good health food. Phycocyanin can improve human immunity, promote animal cell regeneration, inhibit certain cancer cells, etc., and has important medical value. Phycocyanin also has strong fluorescence and is made into a fluorescent probe in molecular biology. Therefore, phycocyanin has a good development prospect, and its wide application has become a research hotspot. [0003] Spirulina is a low-level organism consisting of unicellular or multicellular, and phycocyanin exists in the cell fluid. The natural blue tone of phycocyanin is very good, but its stability is relativ...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/14C07K1/34
CPCC07K14/795
Inventor 胡银银梅元碧倪建辉
Owner WUHAN GREEN FOOD BIOLOGICAL ENG
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