Kit for detecting cancer prognosis

A detection kit and detection reagent technology, applied in the field of medicine and health, can solve the problems of limited kidney cancer and the need to enhance the prediction effect of the model, and achieve the effect of high predictive value

Active Publication Date: 2020-01-21
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the prior art discloses a large number of genes related to renal cancer, the practical application of renal cancer-related genes to assess the prognosis of renal cancer is limited.
The predictive effect of the existing models still needs to be enhanced, and a model that can more accurately predict the risk of renal cancer needs to be established from a large number of renal cancer genes

Method used

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  • Kit for detecting cancer prognosis
  • Kit for detecting cancer prognosis
  • Kit for detecting cancer prognosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Establishment of an assessment model for recurrence and / or survival consequences or prognosis of renal cell carcinoma

[0042] By screening 80 patients with kidney cancer from Fudan University Cancer Hospital as a discovery set, the corresponding gene expression data were extracted by RNA-seq (transcriptome sequencing technology), and the Lasso Cox regression model was used to predict the biochemical recurrence of these patients. related genes. Finally, a genetic decision-making model based on 5 genes was determined and constructed (see figure 1 ). And according to the risk of the decision model, the following scoring formula was used to obtain the risk value, and the patients were divided into high-risk group and low-risk group of renal cancer.

[0043] Risk value=(1.6631×ASPA expression level)+(0.2026×TMEM38B expression level)+(0.1667×PTGR2 expression level)+(-2.3969×TXLNB expression level)+(-2.0618×ADGRE2 expression level).

[0044] The mRNA expression v...

Embodiment 2

[0046] Embodiment 2 is used for the multigene test kit of kidney cancer prognosis

[0047] The multigene kit used for renal cancer prognosis in this embodiment includes:

[0048] Total RNA extraction reagent Trizol;

[0049] Chloroform (chloroform);

[0050] isoamyl alcohol;

[0051] Anhydrous ethanol;

[0052] DEPC water (DD1005);

[0053] Ethylene glycol pyrophosphate (DEPC);

[0054] Anti-RNase solution (RNaseZap);

[0055] Reverse transcription kit;

[0056] iQ SYBR Green Supermix;

[0057] The polynucleotide primer whose sequence is SEQ ID NO: 1-10.

[0058] After using the kit to perform real-time quantitative PCR (qRT-PCR) detection of each gene, the initial data result is represented by the Ct value (cycle threshold), that is, the number of cycles required for the fluorescent signal in each reaction system to reach the set threshold. The Ct value of each sample has a linear relationship with the logarithm of the initial copy number of the sample, and the highe...

Embodiment 3

[0061] Preparation and pretreatment of embodiment 3 sample RNA

[0062] (1) For the tissue RNA extraction experiment, soak the tip and tweezers in DEPC water overnight, then sterilize under high temperature and high pressure, pre-cool the centrifuge at 4°C, and wipe the test bench, pipette gun, gloves, etc. with anti-RNase Zap.

[0063] (2) Tissue homogenate Take 50-100mg tissue sample, mince it slightly with a sterile scalpel blade, put it into a 1.5mL EP tube, add 500μL Trizol reagent, fully homogenize with an electric tissue grinder, and then add 500μL Trizol reagent .

[0064] (3) Add 0.2mL chloroform to every 1mL Trizol reagent homogenate sample, and tightly cap the EP tube. Shake vigorously for 15 seconds, and place at room temperature for 3 minutes. Centrifuge at 12,000 rpm at 4°C for 15 minutes (pre-cool the centrifuge). After centrifugation, the mixed system will be divided into a colorless aqueous phase in the upper layer, a protein in the middle layer and a red p...

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Abstract

The present invention discloses a kit for detecting cancer prognosis. The kit comprises detection reagents capable of detecting expression levels of ASPA, TMEM38B, PTGR2, TXLNB and ADGRE2. The presentinvention provides the kit capable of highly predicting the cancer prognosis.

Description

technical field [0001] The invention relates to the field of medicine and health, in particular to a cancer prognosis detection kit. Background technique [0002] Renal carcinoma is a malignant tumor originating from the urinary tubular epithelial system of the renal parenchyma, and its full academic term is renal cell carcinoma. Renal cancer accounts for about 2% to 3% of adult malignant tumors and 80% to 90% of adult renal malignant tumors. The incidence rate varies from country to country or region in the world. In general, the incidence rate in developed countries is higher than that in developing countries, and in urban areas is higher than in rural areas. There are more men than women. The ratio of male to female patients is about 2:1. The age of onset can vary. Seen in all age groups, the highest incidence age is 50 to 70 years old. According to the statistics of tumor incidence and death in pilot cities and counties in my country by the National Cancer Prevention a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/118
Inventor 叶定伟
Owner FUDAN UNIV SHANGHAI CANCER CENT
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