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Extraction method and application of usnea longissima polysaccharide

An extraction method and technology for growing Usnea, which are applied in the directions of digestive system, drug combination, allergic diseases, etc., can solve the problems that active ingredients are not effectively and efficiently utilized, and the application degree of Usnea is low, and the extraction method is simple and efficient. , Simple and efficient operation, good use effect

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems that the existing usnea has a low application degree, most of which are simply added and utilized usnea, and its main active ingredients have not been effectively and efficiently utilized, the invention provides a kind of usnea long polysaccharide Extraction method and its application

Method used

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  • Extraction method and application of usnea longissima polysaccharide
  • Extraction method and application of usnea longissima polysaccharide
  • Extraction method and application of usnea longissima polysaccharide

Examples

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Effect test

Embodiment 1

[0046] A method for extracting Usnea longus polysaccharide, said method comprising the following steps:

[0047] 1) Use Usnea longissima as raw material, grind it, repeatedly degrease it with acetone for 12 hours, and dry it in an oven to obtain a powder, mix the powder and water at a mass ratio of 1:20 to form a dispersion, and make the dispersion After preheating to 50°C, add cellulase accounting for 1% of the total mass of the dispersion to the dispersion liquid for enzymatic hydrolysis for 4 hours, then raise the temperature to 80°C for leaching for 240 minutes, separate the filter residue and take the supernatant and concentrate it to 30% of the original volume obtain a concentrate;

[0048] 2) Mix the concentrated solution with 95% VOL ethanol aqueous solution at a volume ratio of 1:5 for alcohol precipitation for 12 hours, filter and separate the precipitate and dissolve the precipitate in water, perform 8000r / min centrifugation and dialysis for desalination, after dial...

Embodiment 2

[0053] A method for extracting Usnea longus polysaccharide, said method comprising the following steps:

[0054] 1) Use Usnea longissima as raw material, grind it, repeatedly degrease it with acetone for 12 hours, and dry it in an oven to obtain a powder, mix the powder and water with a mass ratio of 1:18 to form a dispersion, and make the dispersion After preheating to 45°C, add cellulase accounting for 1% of the total mass of the dispersion to the dispersion liquid for enzymatic hydrolysis for 3 hours, then raise the temperature to 75°C for leaching for 270 minutes, separate the filter residue and take the supernatant and concentrate it to 30% of the original volume obtain a concentrate;

[0055] 2) Mix the concentrated solution with 95% VOL ethanol aqueous solution at a volume ratio of 1:4.5 for alcohol precipitation for 12 hours, filter and separate the precipitate and dissolve the precipitate in water, carry out 8000r / min centrifugation and dialysis desalination, after di...

Embodiment 3

[0058] A method for extracting Usnea longus polysaccharide, said method comprising the following steps:

[0059] 1) Use Usnea longissima as raw material, grind it, repeatedly degrease it with acetone for 12 hours, and dry it in an oven to obtain a powder, mix the powder and water at a mass ratio of 1:22 to form a dispersion, and make the dispersion After preheating to 55°C, add cellulase accounting for 1% of the total mass of the dispersion to the dispersion liquid for enzymatic hydrolysis for 5 hours, then raise the temperature to 85°C for leaching for 210 minutes, separate the filter residue, take the supernatant and concentrate it to 30% of the original volume obtain a concentrate;

[0060] 2) Mix the concentrated solution with 95% VOL ethanol aqueous solution at a volume ratio of 1:5.5 for alcohol precipitation for 12 hours, filter and separate the precipitate and dissolve the precipitate in water, perform 8000r / min centrifugation and dialysis for desalination, after dialy...

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Abstract

The invention relates to the field of edible and medicinal processing, and in particular relates to an extraction method and application of usnea longissima polysaccharide. The extraction method comprises the following steps: by using usnea longissima as a raw material, performing grinding, performing degreasing, performing drying to obtain powder, mixing the powder and water to form a dispersionliquid, adding cellulase into the dispersion liquid, performing enzymolysis and performing heating extraction, separating filter residues, and concentrating the supernatant to obtain a concentrated solution; mixing the concentrated solution and an alcohol, performing alcohol precipitation, performing filtering to separate the precipitate, dissolving the precipitate into water, performing centrifugation, performing dialysis desalting, and performing freeze drying on the product obtained after dialysis desalting to obtain crude sugar; and performing deproteinization treatment on the crude sugarby using a Sevage method to obtain a deproteinized solution, performing adsorption on polysaccharide in the deproteinized solution by using an ion exchange column, performing elution on the polysaccharide, after the elution is completed, performing concentration, performing dialysis desalting, and performing freeze drying to obtain the usnea longissima polysaccharide. The method can extract the high-purity usnea longissima polysaccharide; and the obtained product can be directly used for activating intestinal immunity.

Description

technical field [0001] The invention relates to the field of food and drug processing, in particular to a method for extracting usnea polysaccharide and its application. Background technique [0002] Usnea longissima Ach, the English full name is Usnea longissima Ach, it is called Nvluo in "The Book of Songs", and it is called Songluo in "The Pharmacology of Traditional Chinese Medicine". At the same time, it is also called tree hanging ("Heilongjiang Traditional Chinese Medicine") , Asparagus ("Sichuan Traditional Chinese Medicine Annals"), Pine Parasite ("Compendium"), Canopy grass ("Shaanxi Chinese Herbal Medicine"), and dodder, which belong to the plants of the Usnea genus in the Usneaceae family. The growth environment is basically in coniferous and broad-leaved forests, attached to tree trunks or branches. At present, a variety of plants of the genus Lichen have been reported in the world, among which Usnea longus is a common species, and its distribution abroad is ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04A61P1/00
CPCA61P1/00A61P37/04C08B37/0003
Inventor 陈荫王腾董喆孙坤来赵玉勤王斌
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