Positive plasmid for identifying transgenic rice transformant as well as construction method and application thereof

Abstract

The invention discloses a positive plasmid for identifying a transgenic rice transformant as well as a construction method and an application thereof. The positive plasmid comprises a vector skeletonand a screening element, the screening element is composed of 11 rice transformant flanking sequences and two rice internal standard genes, and the two rice internal standard genes are respectively anSPS gene and a PLD gene. The positive plasmid constructed by the invention can be applied to screening and detection of transgenic rice; the method comprises the following steps: taking the positiveplasmid as a positive control plasmid or a positive standard substance for transgenic rice detection; and carrying out common PCR or real-time fluorescent PCR screening detection on transgenic components of a rice sample to-be-detected; the positive plasmid can accurately detect various transgenic rice, meets the requirements of safety supervision and detection work of the transgenic rice, avoidsthe problem that a plurality of positive controls need to be prepared for a plurality of detection targets in the existing detection and identification process, and has the characteristics of wide coverage area, easiness in obtaining a large amount of positive controls, simplicity in preparation and the like.

Description

technical field [0001] The present invention relates to a positive plasmid for identification of transgenic plant transformants, in particular to a positive plasmid for identification of transgenic rice transformants and its construction method. The present invention further relates to the application of the positive plasmid in identifying 11 kinds of transgenic rice transformants, belonging to transgenic Identification field of rice. Background technique [0002] As the staple food of more than half of the world's population, rice is one of the most important food crops. As a new breeding method, transgenic technology has been widely used in crop quality improvement. So far, many studies on transgenic rice such as insect resistance, disease resistance, and herbicide tolerance have been reported. In China, only the genetically modified insect-resistant rice variety Huahui 1 and its hybrid Bt Shanyou 63 have obtained the safety certificate for production and application iss...

AI Technical Summary

Problems solved by technology

These public multi-target positive plasmid molecules can only cover three transgenic lines, and have defects such as narrow coverage, poor applicability and specificity, and need to be improved

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