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Pig sperm malformation rate related molecular genetic marker and application thereof

A molecular genetic marker and sperm deformity technology, applied in the field of molecular genetic biology, can solve the problems of overestimating the marker effect, high computational intensity, unreasonable setting of the significance threshold, etc., so as to reduce the sperm deformity rate and improve the utilization efficiency. Effect

Active Publication Date: 2020-01-24
FOSHAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

Such methods often face problems such as high computational intensity, overestimation of marker effects, and unreasonable setting of significance thresholds.

Method used

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  • Pig sperm malformation rate related molecular genetic marker and application thereof
  • Pig sperm malformation rate related molecular genetic marker and application thereof
  • Pig sperm malformation rate related molecular genetic marker and application thereof

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Experimental program
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Embodiment 1

[0044] Example 1 A screening method for molecular genetic markers related to porcine sperm deformity rate

[0045] 1. Acquisition of Pig Phenotype-Pedigree Data

[0046] The pedigree of the breeding pigs was sorted out, mainly including boar individual number, father, mother and date of birth, and the UltiMateTM CASA (Hamilton Thorne Inc., Beverly, MA, USA) system was used to analyze the fresh semen to obtain the phenotypic data of the sperm deformity rate. For phenotype-genotype association analysis; 2. Genotyping and quality control

[0047] (1) Collect ear tissue samples or blood samples of boars, extract total DNA, and carry out quality inspection on DNA, use GGP50k SNP (GeneSeek, US) chip for genotyping, and obtain SNP marker genotypes covering the whole genome;

[0048] (2) According to the latest version of the pig reference genome (Sscrofa11.1), the physical positions of all SNP markers were updated using the NCBI genome alignment program (https: / / www.ncbi.nlm.nih.gov...

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Abstract

The invention provides a pig sperm malformation rate related molecular genetic marker. The molecular genetic marker site is H3GA0014627, wherein the H3GA0014627 marker site is the 123567821th bp position of the No.4 chromosome of boar, the 123567821th bp position is a mutation site with C being greater than T, and the pig reference genome is Sscrofa11.1. The molecular genetic marker is used for marking boars of different genotypes. The sperm malformation rate has significant difference, T allele significantly reduces the sperm malformation rate, can assist the breeding of boars by detecting the genotype of the H3GA0014627 molecular genetic marker site, selects homozygous boars with the low sperm malformation rate, and reduces the sperm malformation rate, thereby effectively improves the utilization efficiency of the boars.

Description

technical field [0001] The disclosure belongs to the field of molecular genetic biotechnology, and in particular relates to a molecular genetic marker related to the malformation rate of pig sperm and its application. Background technique [0002] Artificial insemination (AI) has been applied in my country since 1954. After decades of technical improvement and integration and national support, artificial insemination technology is becoming more and more mature in my country, and its application in large and medium-sized farms is also basically popular. The advantage of artificial insemination is that it not only improves the utilization rate of good boars, but also timely detects and records the quality of boar semen. At the same time, the health status and reproductive potential of boars can be monitored through the evaluation of semen quality, so as to optimize the individual genetic potential and maximize the reproductive capacity. [0003] In the process of artificial i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 李智丽张楠黄淑坚袁生池仕红喻维维邝伟健
Owner FOSHAN UNIVERSITY
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