Preparation method of bifunctional hybrid film for tumor exosome self-calibration detection

A tumor exosome, self-calibration technology, applied in biochemical equipment and methods, chemical instruments and methods, nanotechnology for sensing, etc. Low cost, convenient operation and good specificity

Active Publication Date: 2020-01-31
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As of the filing date of this patent application, domestic and foreign literature and patent reports on the preparation of BPNSs / Fc / ZIF-67 bifunctional hybrid thin film materials and the self-calibration detection of tumor exosomes based on this thin film material have not been retrieved.

Method used

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  • Preparation method of bifunctional hybrid film for tumor exosome self-calibration detection
  • Preparation method of bifunctional hybrid film for tumor exosome self-calibration detection

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The schematic diagram of the preparation method and detection principle of the self-assembled bifunctional hybrid thin film material based on black phosphorus nanosheets and ferrocene-doped cobalt-based metal-organic framework composites involved in this example is as follows figure 1 As shown, the specific preparation steps are as follows:

[0019] Preparation of Fc / ZIF-67 complex: Weigh a certain amount of Co(NO 3 ) 2 ·6H 2 O and 2-methylimidazole are placed in a mixed solvent of 47mL ethanol and 3mL deionized water, magnetically stirred to form a uniform mixed solution, Co(NO 3 ) 2 ·6H 2The concentrations of O and 2-methylimidazole were adjusted to 0.1 mol / L and 0.8 mol / L, respectively. Put this mixed solution into the electrolytic cell, with Ag / AgCl as the reference electrode, platinum wire as the counter electrode, indium tin oxide ITO as the working electrode, and a constant voltage of -5V, cyclic voltammetry scanning for 100s, the Fc / ZIF-67 The complex is e...

Embodiment 2

[0024] The self-assembled bifunctional hybrid film material based on black phosphorus nanosheets and ferrocene-doped cobalt-based metal-organic framework composites involved in this example, its preparation method and detection principle are the same as in Example 1, and other specific preparation steps are as follows :

[0025] Preparation of Fc / ZIF-67 complex: Weigh a certain amount of Co(NO 3 ) 2 ·6H 2 O and 2-methylimidazole are placed in a mixed solvent of 47mL ethanol and 3mL deionized water, magnetically stirred to form a uniform mixed solution, Co(NO 3 ) 2 ·6H 2 The concentrations of O and 2-methylimidazole were adjusted to 0.3mol / L and 1.2mol / L, respectively. Put this mixed solution into the electrolytic cell, with Ag / AgCl as the reference electrode, platinum wire as the counter electrode, and indium tin oxide ITO as the working electrode, with a constant voltage of -8V, cyclic voltammetry scanning for 200s, the Fc / ZIF-67 The complex is electrodeposited on the s...

Embodiment 3

[0030] The self-assembled bifunctional hybrid film material based on black phosphorus nanosheets and ferrocene-doped cobalt-based metal-organic framework composites involved in this example, its preparation method and detection principle are the same as in Example 1, and other specific preparation steps are as follows :

[0031] Preparation of Fc / ZIF-67 complex: Weigh a certain amount of Co(NO 3 ) 2 ·6H 2 O and 2-methylimidazole are placed in a mixed solvent of 47mL ethanol and 3mL deionized water, magnetically stirred to form a uniform mixed solution, Co(NO 3 ) 2 ·6H 2 The concentrations of O and 2-methylimidazole were adjusted to 0.5 mol / L and 1.5 mol / L, respectively. Put this mixed solution into the electrolytic cell, with Ag / AgCl as the reference electrode, platinum wire as the counter electrode, indium tin oxide ITO as the working electrode, and a constant voltage of -10V, cyclic voltammetry scanning for 400s, the Fc / ZIF-67 The complex is electrodeposited on the sur...

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Abstract

The invention discloses a preparation method of a bifunctional hybrid film for tumor exosome self-calibration detection. A bifunctional hybrid film, namely aptamer-BPNSs/Fc/ZIF-67/ITO, is constructedon the basis of simple self-assembly of black phosphorus nanosheets BPNSs, an aptamer and a ferrocene Fc doped cobalt-based metal organic framework ZIF-67 compound on an indium tin oxide ITO electrode. The aptamer marked by methylene blue MB is specifically combined with the CD63 protein to capture protein accurately. The protein is a specific biomolecule carried by breast cancer MCF-7 cell exosome, and realizes detection for the tumor cell exosome. MB is used as a response signal, Fc is used as a reference, and a self-calibration sensor for quantitative detection of the tumor exosome is constructed. Compared with the prior art, the exosome self-calibration detection method is convenient to operate, high in sensitivity, low in cost and high in specificity, can be used as a novel exosome self-calibration detection method, and is used for quantitative detection for exosomes in biomedical samples.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of functional hybrid thin film materials and tumor exosome sensors, in particular to a dual-functional hybrid thin film self-assembled based on black phosphorus nanosheets and ferrocene-doped cobalt-based metal-organic framework complexes The preparation method of the material, the film material prepared by it can be used for the precise capture of CD63 transmembrane protein and the self-calibration detection of breast cancer exosomes. Background technique: [0002] Exosomes are extracellular vesicles with a size of 50–100 nm released from polyvesicles through the endolysogenic pathway, which carry a large number of biopolymers from parent cells, including transmembrane and cytoplasmic proteins, mRNA, DNA and micro-RNA etc. Exosomes act as messengers mediating intercellular information and play an important role in detecting changes in physiological states associated with diseases, especiall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/327G01N27/31
CPCG01N27/31G01N27/3277G01N27/3278G01N27/4163B82Y15/00B82Y30/00B82Y40/00C07H21/00C09K11/58G01N33/5438G01N33/57415G01N33/57488G01N2333/70596B05D1/00C12N15/115C12N2310/16G01N33/57484
Inventor 桂日军孙玉娇金辉
Owner QINGDAO UNIV
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