Purpose of porcine interleukin 11 in resisting porcine epidemic diarrhea virus infection

An interleukin, cell technology, applied in the field of biotechnology genetic engineering
CN110746501AActive Publication Date: 2020-02-04NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2020-02-04

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Abstract

The invention relates to a porcine interleukin 11, and a purpose of the porcine interleukin 11 in resisting porcine epidemic diarrhea virus infection. After porcine epidemic diarrhea virus infection happens, a piglet intestinal tract secretes cell factor porcine pIL-11 which is obviously improved, and the expression of the cell factor exhibits high correlation with a virus infection level. The invention discloses a prokaryotic expression plasmid for constructing a recombinant pIL-11, a transformed escherichia coli BL21, and a specific method for obtaining a high-purity recombinant pIL-11 through purification. The recombinant pIL-11 can inhibit the infection of the PEDV (Porcine epidemic diarrhea virus) for a host cell, and has obvious dose dependency. The recombinant pIL-11 activates a STAT3 (signal transducer and activator of transcription 3) signal to inhibit host cell apoptosis induced after PEDV infection happens so as to perform a function of resisting virus infection. The porcinesource IL-11 has a function of resisting virus infection and alleviating piglet intestinal tract injury, and is expected to become an effective medicine for resisting PEDV intestinal tract infectionin the future.
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Description

technical field

[0001] The invention belongs to the field of biotechnology genetic engineering, and relates to the antiviral use of porcine interleukin-11, in particular to the use of porcine interleukin-11 in resisting porcine epidemic diarrhea virus infection. Background technique

[0002] 1. Research progress on porcine interleukin-11

[0003] Interleukin IL-11 (Interleukin-11, IL-11) is a member of the IL-6 family, which can be produced by various cells in the body, such as immune cells, fibroblasts, smooth muscle cells, endothelial cells, epithelial cells and bone marrow stromal cells Secreted. The initial research on the physiological role of IL-11 mainly focused on its regulation of hematopoietic function, T and B lymphocyte immune function, and nervous system development. Some studies in recent years have shown that IL-11 also plays an important role in the maintenance of the intestinal mucosal barrier. It can play anti-apoptosis, promote proliferation, inhibit inf...

Claims

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