Preparation method of fermented rice and bean clear liquid composite beverage
A fermented, bean clear liquid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low content of aglycon-type isoflavones, easy acidity of wine liquid, and low utilization rate of bean clear liquid And other issues
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Embodiment 1
[0025] This embodiment includes the following steps:
[0026] (1) Preparation of Rhizopus oligosporus strain: firstly activate and cultivate Rhizopus oligosporus on the PDA slope until the spores mature, pick 2 loops of the activated Rhizopus oligosporus with an inoculation loop, and inoculate it into the PD liquid medium , 30 ℃, 160 rpm conditions for 4 days, to obtain Rhizopus oligosporus strain;
[0027] In step (1), the Rhizopus oligosporus is isolated from Tempe, a commercially available fermented soybean product. The separation method is: put 5g of fresh Tempe into 10mL sterile water, shake for 5min, use a sterile pipette to draw 0.1mL suspension and apply it on a PDA medium plate, cultivate at 30℃ for 7 days, and wait until the Rhizopus oligosporus spores mature Afterwards, the conidia were picked and inoculated on the PDA medium slant, cultivated at 30°C for 7 days, and the Rhizopus oligosporus slant strain was obtained.
[0028] (2) Pretreatment and first fermentation of r...
Embodiment 2
[0034] This embodiment includes the following steps:
[0035] (1) Preparation of Rhizopus oligosporus: Activate and cultivate the Rhizopus oligosporus on the PDA slope until the spores mature. Use an inoculating loop to pick 3 loops of the activated Rhizopus oligosporum and inoculate it into the PD liquid medium. Cultivate for 3 days at 30°C and 160 rpm to obtain Rhizopus oligosporus;
[0036] In step (1), the Rhizopus oligosporus is obtained through commercial channels;
[0037] (2) Pretreatment and first fermentation of raw rice: mainly including washing, soaking, steaming, cooling, mixing and fermentation, to obtain the product after the first fermentation;
[0038] The specific operation is as follows: Take 500g of commercially available indica rice, wash and soak in water for 2h, remove and drain the water; then steam the rice. During the process of steaming, start timing after the water boils. When timing is 15 minutes, spray evenly on the surface of the rice at a temperature of...
Embodiment 3
[0043] This embodiment includes the following steps:
[0044] (1) Preparation of Rhizopus oligosporus strain: Activate and culture Rhizopus oligosporus on the PDA slope until the spores mature, pick up 2 loops of activated Rhizopus oligosporus with an inoculation loop, and inoculate it into PD liquid medium. Cultivate for 5 days at 30°C and 160 rpm to obtain Rhizopus oligosporum solution;
[0045] In step (1), the Rhizopus oligosporus is isolated from Tempe, a commercially available fermented soybean product. The separation method is: put 5g of fresh Tempe into 15mL sterile water, shake for 5min, use a sterile pipette to draw 0.1mL suspension and spread it on the PDA medium plate, cultivate at 30℃ for 7d, at least the meristem of Rhizopus The spores are mature; the conidia are picked and inoculated on the PDA medium slope, and cultivated at 30°C for 7 days to obtain the Rhizopus oligosporus slope.
[0046] (2) Pretreatment and first fermentation of raw rice: mainly including washin...
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