Device for capturing fetal nucleated red blood cells (NRBCs) from parent bodies in vivo and in situ and application of device

Abstract

The invention discloses a device for capturing fetal nucleated red blood cells (NRBCs) from parent bodies in vivo and in situ and application of the device. The device comprises a push-pull device, ahollow tube container, a puncture head and a polymer guide wire, wherein two ends of the hollow tube container are open, one end is connected with the puncture head, the push-pull device is placed atthe other end, the push-pull device makes seal contact with the inner wall of the hollow tube container and can slide along the inner wall of the hollow tube container, and the polymer guide wire penetrates through the puncture head and is connected with the bottom of the push-pull device. The device of the invention can make contact with peripheral blood of parent bodies in a shorter time, capture rare fetal NRBCs and capture the fetal NRBCs in vivo and in situ, the problems that a large amount of peripheral blood of parent bodies is drawn, low-efficiency methods such as centrifugation are used for enriching and separating the fetal NRBCs, which are time-consuming and expensive are solved, and the method is low in cost, not time-consuming and high in capture efficiency.

Description

Technical field [0001] The invention relates to a device for capturing fetal eukaryotic red blood cells in situ from a mother body and an application thereof, and belongs to the technical field of medical devices for capturing fetal nucleated red blood cells. Background technique [0002] Cells with less than 100 cells per milliliter of blood are called rare cells. Many types of rare cells have important research value. Among them, circulating tumor cells (CTCs) and fetal nucleated red blood cells (NRBCs) ) Has broad prospects in clinical diagnosis and treatment, with extensive research. Fetal nucleated red blood cells contain complete genetic information of the fetus, which is convenient for genetic and molecular biological analysis. The content of maternal peripheral blood in the early pregnancy is more abundant than other fetal cells. Therefore, NRBCs have important research significance in the field of fetal prenatal diagnosis. NRBCs genes can be tested to diagnose whether t...

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Problems solved by technology

[0004] At present, most of the methods for separating and enriching NRBCs are in vitro capture, which requires a lot of peripheral blood, which is difficult to achieve in situ capture, the sample volume is small, and the amount of blood that can effectively contact the mother's periphery is small; and because NRBCs in the mother's peripheral blood The content is small (less than 100 cells per milliliter), and the uncertainty of in vitro capture is higher than that of in situ capture in vivo; moreover, in vitro capture needs to be carried out in a specific experimental site, and it is difficult to carry out non-destructive processing of captured cells. Preservation or transportation is not conducive to downstream genetic testing and analysis

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