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Tuna red meat ACE inhibitory peptide and preparation method thereof

A tuna and inhibitory peptide technology, applied in the field of tuna red meat ACE inhibitory peptide and preparation thereof, can solve the problems of high cost of blood pressure-lowering peptide, unsuitable for industrial production, low blood pressure lowering activity, etc., and achieves good inhibitory activity and low preparation cost. , the effect of high efficiency

Active Publication Date: 2020-02-07
舟山领鲜海洋生物科技有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to overcome the problems of high cost, low efficiency, inapplicability to industrial production, and low antihypertensive activity of existing antihypertensive peptides, the present invention proposes a tuna red meat ACE inhibitory peptide and a preparation method thereof

Method used

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  • Tuna red meat ACE inhibitory peptide and preparation method thereof
  • Tuna red meat ACE inhibitory peptide and preparation method thereof
  • Tuna red meat ACE inhibitory peptide and preparation method thereof

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Embodiment 1-15

[0041] Embodiment 1-15: a preparation method of tuna red meat ACE inhibitory peptide, comprising the following steps:

[0042] 1) Thaw the tuna red meat, dry it to remove water, then add ethyl acetate to the tuna, immerse it to degrease, rotate it, dry it, and crush it for later use;

[0043] 2) adding distilled water to the pretreated red meat powder, adjusting the pH value to 6-8, adding 1-3 wt% alkaline protease, and enzymatically hydrolyzing at 45-65°C for 4 hours to obtain an enzymolyzed solution;

[0044] 3) Heat the enzymatic hydrolysis solution in a water bath at 100°C for 10 minutes to inactivate the enzyme, then centrifuge at 4000r for 20 minutes, take the supernatant to obtain the enzymatic hydrolysis solution inactivating the enzyme, then freeze-dry to obtain the polypeptide powder, and measure the ACE inhibitory activity;

[0045]4) Use a 3.5KDa ultrafiltration membrane to carry out ultrafiltration classification on the enzymatic hydrolyzate of tuna red meat, and ...

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Abstract

The invention provides tuna red meat ACE inhibitory peptide and a preparation method thereof, and relates to the field of preparation of ACE inhibitory peptide. The peptide fragment amino acid sequences of the tuna red meat ACE inhibitory peptide are Met-Trp-Asn, Met-Glu-Lys-Ser, Met-Lys-Lys-Ser or Leu-Pro-Arg-Ser. The preparation method comprises the following steps of (1) unfreezing the tuna redmeat, performing drying and performing degreasing for standby application; (2) performing enzymolysis on red meat powder after pretreatment to obtain enzymatic hydrolysate; (3) performing enzyme deactivation treatment on the enzymatic hydrolysate to obtain enzyme deactivation enzymatic hydrolysate, performing freeze drying to obtain polypeptide powder, and testing ACE inhibitory activity; and (4)subsequently, performing ultrafiltration, performing column chromatography, performing high efficiency liquid phase chromatographic purification, and performing amino acid sequencing to prepare the tuna red meat ACE inhibitory peptide. The prepared tuna red meat ACE inhibitory peptide is good in inhibitory activity, low in preparation cost, high in efficiency and suitable for industrial production.

Description

technical field [0001] The invention relates to the field of preparation of ACE inhibitory peptides, in particular to a tuna red meat ACE inhibitory peptide and a preparation method thereof. Background technique [0002] The occurrence of hypertension is closely related to the disorder of renin-angiotensin system (RAS) and kallikrein-kinin system (KKS). Angiotensin-converting enzyme plays a role in regulating blood pressure by converting angiotensin Ⅰ (Ang Ⅰ) into the octapeptide angiotensin Ⅱ (Ang Ⅱ ), which has a potent vasoconstrictive effect, and by degrading bradykinin, which has a vasodilation effect. critical use. Antihypertensive peptides can form competitive inhibition on the substrate of angiotensin-converting enzyme, and affect the generation of Ang II, which has a strong vasoconstrictive effect, so as to achieve the purpose of lowering blood pressure. Therefore, angiotensin-converting enzyme inhibition plays an important role in antihypertensive drugs target. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/083C07K5/103A61P9/12C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16
CPCA61P9/12C07K5/081C07K5/101C07K5/1013C12P21/06
Inventor 赵玉勤
Owner 舟山领鲜海洋生物科技有限公司
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