Large-scale cultivation method of all-female Nibea albiflora
A breeding method and technology of yellow croaker, applied in fish farming, climate change adaptation, additional food elements, etc., can solve the problems of inapplicability to large-scale production of all-female populations and low survival rate of seedlings, so as to promote gender control The effects of research and application, easy operation, and easy scale production
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[0034] The large-scale cultivation method of whole female yellow croaker of the present invention comprises the following steps:
[0035] 1. Induction of gynogenetic diploid in yellow croaker
[0036] (1) Inactivation of genetic material in the sperm of the sea bream: using 10 female croakers and 5 males (collected from the sea area of Zhoushan) cultivated by the Key Laboratory of Seawater Aquaculture in Zhejiang Province, after artificial induction Sperm and eggs are collected.
[0037] Dilute the semen of the stone seabream with sperm dilution (by mass ratio, NaCl 0.75%, KCl 0.02%, CaCl 2 0.04%, the balance is water, pH value 8.2) diluted 30 times, spread it in a petri dish, adjust the thickness of the dilution, and use 45 mJ / cm 2 The intensity of ultraviolet light was irradiated for 45 s to inactivate the genetic material of sperm.
[0038] (2) Fertilization of unfertilized eggs and inactivated sperm: Mix the inactivated crocodile semen irradiated by ultraviolet rays w...
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