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Large-scale cultivation method of all-female Nibea albiflora

A breeding method and technology of yellow croaker, applied in fish farming, climate change adaptation, additional food elements, etc., can solve the problems of inapplicability to large-scale production of all-female populations and low survival rate of seedlings, so as to promote gender control The effects of research and application, easy operation, and easy scale production

Active Publication Date: 2020-02-11
MARINE FISHERIES RES INST OF ZHEJIANG
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Problems solved by technology

However, there is no report on the large-scale cultivation technology of all-female croaker at home and abroad.
[0004] The croaker is a fish with an XY sex determination system, and the generation of an all-female population can be achieved in two different ways: the first method is to use estrogen treatment to induce all-female fish, but this method is due to the use of steroids. Therefore, hormones cannot be promoted in production; the second method is to induce gynogenetic seedlings, but this technology requires external shocks during embryogenesis, and the survival rate of seedlings is low, so it is not suitable for large-scale all-female populations Production

Method used

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  • Large-scale cultivation method of all-female Nibea albiflora

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Embodiment 1

[0034] The large-scale cultivation method of whole female yellow croaker of the present invention comprises the following steps:

[0035] 1. Induction of gynogenetic diploid in yellow croaker

[0036] (1) Inactivation of genetic material in the sperm of the sea bream: using 10 female croakers and 5 males (collected from the sea area of ​​Zhoushan) cultivated by the Key Laboratory of Seawater Aquaculture in Zhejiang Province, after artificial induction Sperm and eggs are collected.

[0037] Dilute the semen of the stone seabream with sperm dilution (by mass ratio, NaCl 0.75%, KCl 0.02%, CaCl 2 0.04%, the balance is water, pH value 8.2) diluted 30 times, spread it in a petri dish, adjust the thickness of the dilution, and use 45 mJ / cm 2 The intensity of ultraviolet light was irradiated for 45 s to inactivate the genetic material of sperm.

[0038] (2) Fertilization of unfertilized eggs and inactivated sperm: Mix the inactivated crocodile semen irradiated by ultraviolet rays w...

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Abstract

The invention provides a large-scale cultivation method of all-female Nibea albiflora. The method mainly includes the steps of firstly, inducing the gynogenesis diploid of Nibea albiflora; inducing and identifying pseudo-male Nibea albiflora; thirdly, allowing the pseudo-male Nibea albiflora to mate with normal female Nibea albiflora to reproduce all-female Nibea albiflora. The method has the advantages that aromatase inhibitor letrozole is used to induce pseudo-male Nibea albiflora reproduction on the basis that the Nibea albiflora gynogenesis technology is built, the mating of the pseudo-male Nibea albiflora and the normal female Nibea albiflora is utilized to obtain the all-female Nibea albiflora for the first time; the built large-scale all-female Nibea albiflora cultivation method issimple to operate, safe, efficient and the like, the cultivated all-female Nibea albiflora is fast in growth, stable in character and the like, and strong technical support is provided for the realizing of Nibea albiflora unisexual cultivation.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a large-scale cultivation method of whole female yellow croaker. Background technique [0002] In aquaculture, because the females of many fish grow faster and have larger individuals, it is of great significance to cultivate all-female populations to increase aquaculture production, shorten the aquaculture cycle, and increase economic benefits. The breeding of all-female fish has received great attention worldwide. Japan has bred all-female fish by combining gynogenesis and hormone-changing sex, which has been successful in salmon, rainbow trout, flounder and other fish. my country has also made some achievements in fish sex control, and has established methods for gynogenesis and pseudo-male fish induction in various fish species, such as the realization of parthenogenesis in tilapia, flounder and other fish , greatly improving the breeding efficiency. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A23K50/80A23K20/137
CPCA23K20/137A23K50/80A01K61/10Y02A40/81
Inventor 徐冬冬陈睿毅陶勇秦泽倩田璐竺奇慧王立改谭朋汪波
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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