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Oil/fat liposome and preparation method thereof

A liposome and oil technology, which is applied in the direction of liposome delivery, edible oil/fat, and oil-containing food ingredients, etc., can solve the problems of poor comprehensive usability of oil liposomes and cannot meet the use requirements, and achieve increased stability , inhibit cholesterol absorption, the effect of compact structure

Active Publication Date: 2020-02-14
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, what prepare lipid liposome majority use is soybean phospholipid, egg phospholipid and synthetic phospholipid (as DPPC; Palmitoyl-lecithin) etc., but never use the polar lipid from milk to prepare lipid liposome, And adopt soybean phospholipids, egg phospholipids and synthetic phospholipids to prepare lipid liposomes with poor comprehensive usability, can not meet the use requirements

Method used

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  • Oil/fat liposome and preparation method thereof
  • Oil/fat liposome and preparation method thereof
  • Oil/fat liposome and preparation method thereof

Examples

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preparation example Construction

[0037] The embodiment of the present invention also provides a kind of preparation method of lipid liposome as above, specifically comprises the following steps:

[0038] (1) Grease, fat globule membrane phospholipids, organic solvent, surfactant and PBS buffer are prepared as required for subsequent use; surfactant includes Tween-80, pH of PBS buffer=7.0~7.4, pH of PBS buffer The concentration is 0.005~0.01mol / L; the organic solvent includes anhydrous ether or chloroform; the mass ratio of oil and fat globule membrane phospholipid is 2:8; the oil, fat globule membrane phospholipid, anhydrous ether, surfactant and PBS buffer The mass-volume ratio is (100-200) mg: (800-900) mg: (16.7-25) mL: (200-240) mg: 20 mL;

[0039] (2) Mix the fat and fat globule membrane phospholipids evenly to obtain mixture A, add an organic solvent to mixture A, shake and dissolve, and mix ultrasonically to obtain a mixed solution; the ultrasonic power is 70-90W, the ultrasonic temperature is 25°C, an...

Embodiment 1

[0047] Preparation of lipid liposomes by thin film ultrasonic dispersion

[0048] Weigh 900 mg of fat globular membrane phospholipids and 100 mg of fat for wall material, add 20 mL of anhydrous ether to fully shake and dissolve the mixture, and ultrasonically mix the solution for 1 min; use a rotary evaporator to remove the organic solvent of the mixed solution by rotary evaporation at 45 °C. Until a pale yellow liposome film is formed on the wall, then dry in a drying oven for 4 hours to completely remove the organic solvent; add 20 mL of PBS buffer solution of pH = 7.4, 0.005 mol / L containing 240 mg Tween 80, at 45 °C Rotate and hydrate at normal pressure for 45 minutes to wash the membrane to form a milky white uniform liposome solution;

[0049] Then the swirling solution of the lipid liposome is ultrasonically 20min in a water bath under 80W power, so that the film is swollen and hydrated, and finally, the premixed crude emulsion is homogenized with a high-pressure micro-...

Embodiment 2

[0050] Embodiment 2 film ultrasonic dispersion method prepares grease liposome

[0051] Weigh 800 mg of fat globular membrane phospholipids and mix them with 200 mg of oil, add 20 mL of anhydrous ether to fully shake and dissolve the mixture, and ultrasonically mix the solution for 1 min; use a rotary evaporator to remove the organic solvent of the mixed solution at 45 ° C until the wall A light yellow liposome film was formed on the liposome, and then dried in a drying oven for 4 hours to completely remove the organic solvent; add 20 mL of PBS buffer solution containing 240 mg Tween 80, pH = 7.0, 0.005 mol / L, at 45 °C under normal pressure Rotate and hydrate for 45 minutes to wash the membrane to form a milky white uniform liposome solution;

[0052] Then the swirling liquid of lipid liposomes is ultrasonicated in a water bath under 70W power for 30min, so that the film is swollen and hydrated, and finally, the premixed crude emulsion is homogenized with a high-pressure micro...

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Abstract

The invention discloses an oil / fat liposome and a preparation method thereof. The oil / fat liposome is characterized in that the oil / fat liposome comprises oil / fat and fat globule membrane phospholipidcoated on the surface of the oil / fat; the oil / fat liposome is prepared by a mutual coordination between polar lipid from milk (fat globule membrane phospholipid) and the oil / fat, thus, insurmountableproblems of traditional processes of the oil / fat in pharmaceutical preparations or food processing can be effectively solved, application quality of the oil / fat is greatly improved, and application value of the milk polar lipid is realized.

Description

technical field [0001] The invention relates to the technical field of liposome preparation, in particular to a lipid liposome and a preparation method thereof. Background technique [0002] Oils and fats are esters formed by various higher fatty acids (such as stearic acid, palmitic acid, oleic acid and linoleic acid, etc.) 6(n-6) and other polyunsaturated fatty acids (PUFA). At present, it is widely believed that oil plays an important role in reducing the risk of disease (prevention of cardiovascular disease, hypertension, diabetes) and improving consumer health (promoting brain and eye development during infant growth), so it is widely used in the food industry and The field of medical engineering has high application value. [0003] However, due to the poor compatibility of oils and fats in water-based products, they are prone to degradation and oxidation reactions, and are prone to isomerization reactions under the catalysis of light and trace metals, resulting in ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/115A23D9/04A23L5/30A23P10/30A61K9/127A61K47/24
CPCA23L33/115A23D9/04A23L5/32A23P10/30A61K9/127A61K47/24A23V2002/00A23V2200/308A23V2200/3262A23V2250/18A23V2300/48
Inventor 李晓东张宇刘璐朱启鹏常淤浩
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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