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Enzyme composition and its preparation method

An enzyme composition and technology for active substances, applied in the field of enzyme activity, can solve problems such as affecting enzyme activity, a large number of experiments, and complicated operations.

Active Publication Date: 2022-04-29
JIANGSU VOCATIONAL COLLEGE OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The inventors found in the process of realizing the present invention that the disadvantage of the first method for improving the thermostability of enzymes is that naturally occurring enzymes with thermostability cannot be mass-produced on a large scale and do not have economic benefits
The disadvantage of the second method for improving the thermostability of enzymes is that the enzymes improved by means of molecular biotechnology need to mutate themselves, and then a large number of mutated enzymes are screened to obtain enzymes with high thermostability. A large number of experiments are required, the operation is cumbersome, and the activity of the enzyme obtained by screening is relatively low
Not only that, mutations can also affect the specificity of the enzyme
The disadvantage of the third method to improve the thermostability of the enzyme is that the existing additives usually have high salt concentration, which affects the activity of the enzyme
In addition, improving the thermal stability of enzymes through immobilization methods will also affect the activity of enzymes

Method used

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  • Enzyme composition and its preparation method
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  • Enzyme composition and its preparation method

Examples

Experimental program
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Effect test

preparation example Construction

[0035] The preparation of embodiment 1 cicada flower active substance (CAF)

[0036] Take the cicadae mycelium and inoculate it on the plate medium, cultivate it at 25°C for 18 days, then inoculate it into the Erlenmeyer flask, and cultivate it at 25°C for 7 days, and finally inoculate the cultured cicadae mycelium into the fermenter, and cultivate it at 25°C 3d, finally forming a fermentation liquid containing the active substance of cicadae, which is the fermentation liquid of cicadae mycelium.

[0037] The distilled fermented liquid of the active substance of cicada japonica was dried at 60° C. for 24 hours, then ground, and finally sieved through a 0.5 cm sieve to obtain CAF powder. Add 10mL, 100mmol / L sodium acetate buffer solution to every gram of CAF powder to obtain CAF suitable solution.

Embodiment 2

[0038] The test of embodiment 2 phytase thermostability

[0039] 1) Preparation and treatment of phytase CAF composition

[0040] Add 1 g of phytase to 4 mL of 100 mmol / L sodium acetate buffer to obtain a phytase solution. Get three parts of 1mL phytase solution, respectively add 2g, 5g, 10g of CAF powder prepared in Example 1 to obtain 1:2, 1:5, 1:10 phytase CAF composition, place it in the oven At 60°C, dry for 1 hour to obtain 1:2, 1:5, 1:10 phytase CAF composition test samples.

[0041] 2) Preparation and processing of the control composition

[0042] Add 1g of phytase to 4mL, 100mmol / L sodium acetate buffer to obtain a phytase solution, take 1mL of the phytase solution, place it in an oven, and dry it for 1 hour at 60°C to obtain a phytase control group sample .

[0043] 3) Phytase activity test

[0044] Take 1g of the phytase CAF composition test sample and the phytase control group sample and add 4mL, 100mmol / L sodium acetate buffer solution to make the test soluti...

Embodiment 3

[0047] The test of embodiment 3 cellulase thermostability

[0048] 1) Preparation and treatment of cellulase CAF composition

[0049]Add 1 g of cellulase to 4 mL of 100 mmol / L sodium acetate buffer to obtain a cellulase solution. Take 1mL of cellulase solution, add 2g of CAF powder prepared in Example 1 to obtain a 1:2 cellulase CAF composition, place it in an oven, and dry it for 1 hour at 60°C to obtain a 1:2 Test samples of the cellulase CAF composition.

[0050] 2) Preparation and processing of the control composition

[0051] Add 1 g of cellulase to 4 mL of 100 mmol / L sodium acetate buffer to obtain a cellulase solution. Take 1mL of cellulase solution, place it in an oven, and dry it at 60°C for 1 hour to obtain the sample of cellulase control group.

[0052] 3) Cellulase activity test

[0053] Take 1g of the cellulase CAF composition test sample and the cellulase control group sample and add 4mL, 100mmol / L sodium acetate buffer solution to make the test solution, an...

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Abstract

The invention belongs to the technical field of enzyme activity, and relates to an enzyme composition and a preparation method thereof. The enzyme composition comprises: enzymes and cicadae active substances. The enzyme composition provided by the invention can improve the thermostability of the enzyme without affecting the activity of the enzyme.

Description

technical field [0001] The invention belongs to the technical field of enzyme activity, and more specifically relates to an enzyme composition and a preparation method thereof. Background technique [0002] Enzymes are widely used as catalysts in various production processes. In the application process, catalysts are usually accompanied by high-temperature treatment, especially in industrial manufacturing, but high-temperature treatment will destroy the structure of the enzyme and cause the enzyme to lose its catalytic activity. For example, in the feed preparation process, when the temperature reaches 90 ° C, 2 ~ The enzyme will be inactivated in 5 minutes. [0003] Existing methods for improving the thermostability of enzymes mainly include: the first is to screen enzymes with natural thermostability; the second is to use molecular technology to improve the thermostability of enzymes; the third is to use additives. [0004] In the process of realizing the present inventi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96C12N1/14C12P1/02C12N9/16C12N9/42C12N9/26C12R1/645
CPCC12N9/96C12N1/14C12P1/02C12N9/16C12N9/2437C12N9/2414C12Y301/03008C12Y301/03026C12R2001/645C12N1/145
Inventor 张小蒙张莉杨恒月周敏
Owner JIANGSU VOCATIONAL COLLEGE OF MEDICINE
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