Method for increasing old tree cutting survival rate

A technology of survival rate and ancient trees, applied in the biological field, can solve the problems of low survival rate of cuttings of ancient trees, achieve the effect of improving the rooting ability of cuttings, accelerating cell division, and promoting rooting

Active Publication Date: 2020-02-18
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the embodiment of the present invention provides a method for improving the survival rate of cuttings of ancient trees to solve the problem of low survival rate of cuttings of ancient trees in the prior art

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  • Method for increasing old tree cutting survival rate
  • Method for increasing old tree cutting survival rate
  • Method for increasing old tree cutting survival rate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the cutting condition of the ancient tree of orientalis arborvitae

[0026] 1. Screening matrix ratio

[0027] Different tree species are suitable for different substrates. Fast-growing tree species require a substrate with a large amount of water, while tree species with a long rooting time require a substrate with less water.

[0028] In this example, peat soil: perlite = 3:7 (ck); peat soil: perlite = 3:7 (add slow-release fertilizer); peat soil: perlite = 3:7 (add slow-release fertilizer later) ; Peat soil: perlite = 2:8 (ck); peat soil: perlite = 2:8 (add slow-release fertilizer); peat soil: perlite = 2:8 (add slow-release fertilizer later).

[0029] Taking the 5-year-old Arborvitae as a contrast, the 100-year-old Arborvitae of the Chinese Academy of Forestry was used for the following experiments: After disinfecting the cuttings of ancient Arborvitae trees of different ages, they were used in aqueous solutions containing different hormones. The aque...

Embodiment 2

[0033] Embodiment 2, the influence of plant growth regulator on cutting rooting rate

[0034] When the cuttings of ancient trees take root, they need certain nutrients, mainly carbohydrates and nitrogen compounds. Treatment of cuttings with sucrose aqueous solution has a better rooting effect. In addition, sodium ligninate (ASL), as a plant growth regulator, has the functions of promoting plant wound healing, rooting of cuttings, increasing seed germination rate and accelerating seedling growth, and can improve the stress resistance of forest tree seedlings. Pre-soaking the cuttings with a certain concentration of ASL can significantly promote rooting within a certain concentration range. The mixed use of ASL and NAA:IBA=1:1 can not only promote wound healing but also promote adventitious root formation. Therefore, an experiment was designed to explore the optimal ASL concentration suitable for cutting propagation of Arborvitae arborvitae, and the rooting situation after tre...

Embodiment 3

[0038] Embodiment 3, ancient tree cuttings are each treated with plant growth regulators

[0039] Carbohydrates are plant energy stores, carbon sources for secondary substances such as flavonoids, and participate in stress responses. The soluble sugar and starch contents of cuttings of Arborvitae arborvitae of different tree ages were not significantly different, but the results were consistent with the results that the initial carbohydrate content of cuttings could meet the requirement of adventitious root formation. In this example, the soluble sugar and starch content of the cuttings of Arborvitae of all ages increased to varying degrees during the callus formation stage, so adding exogenous nutrients during the cutting process of ancient Arborvitae trees may help to improve rooting. Rate. The rooting time of arborvitae is long, and there is a lot of rain in summer, and the substrate is easy to store water, causing the cuttings to rot prematurely. Therefore, timely supple...

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Abstract

An embodiment of the invention discloses a method for increasing old tree cutting survival rate. The method includes: cultivating old tree cutting after being treated by a plant growth regulator untilcallus comes up, vertically carving the callus, and sticking the old tree cutting into a matrix added with slow-release fertilizer for cultivating and rooting, wherein the plant growth regulator comprises NAA, IBA and sodium lignocerate, the matrix comprises turf and a water retention matrix according to a mass ratio of 2:8, and the water retention matrix comprises more than one of vermiculite, perlite, pumice stone and ceramsite. By experimenting with differently proportioned matrixes, tree seeds long in rooting time need matrixes low in water storage volume, so that proper matrix proportionis selected to increase old tree cutting survival rate and shorten old tree cutting rooting time.

Description

technical field [0001] The embodiment of the present invention relates to the field of biotechnology, in particular to a method for improving the survival rate of ancient tree cuttings. Background technique [0002] Cutting propagation is very important to preserve the excellent genetic resources of ancient trees, such as precious longevity and stress resistance genes. It is difficult for cypress trees to root cuttings, but it is even more difficult for ancient trees. As the tree age increases, the rooting rate of cuttings drops sharply. This is mainly because the cutting of ancient trees takes a long time, and the requirements for the substrate need to be moisturized and very permeable. It is easy to be invaded by pathogens in the matrix for a long time, and the nutrients in the body are also exhausted during the long rooting process, thereby affecting the reduction of auxin and other substances that promote the occurrence of root primordia. In the past, the cuttings were...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01G24/28A01G24/15A01G24/10A01G13/00A01N43/38A01N37/10A01N41/04A01P21/00
CPCA01G2/10A01G24/28A01G24/15A01G24/10A01G13/00A01N43/38A01N37/10A01N41/04
Inventor 常二梅江泽平张进赵秀莲刘建锋
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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