A kind of preparation method of spirulina bioluminescence metal organic framework compound

Abstract

The invention discloses a method for preparing a spirulina bioluminescence metal organic framework compound, the method comprising preparation of a bioluminescence component and preparation of a spirulina biofluorescence γ-CD-MOFs solution, and a spirulina biofluorescence γ-CD-MOFs solution The preparation is to stir and dissolve the mixed aqueous solution of γ-cyclodextrin and metal ligand until clear, filter the filtrate, add the filtrate and bioluminescent components into a small beaker to obtain a mixed solution, and then place the small beaker in the center of the large beaker place, inject methanol or absolute ethanol into the large beaker and the level of the injected methanol or absolute ethanol is higher than the liquid level of the mixture in the small beaker and seal it with a fresh-keeping bag, and place it at room temperature or in an oven at 60°C for 2 to 7 days The spirulina bioluminescent metal-organic framework compound is obtained. The obtained spirulina bioluminescent metal organic framework compound has blue and red fluorescence, is easy to operate, non-toxic, and can be produced in large quantities.

Description

technical field [0001] The invention relates to a preparation method of a spirulina bioluminescence metal organic framework compound. It is prepared by introducing spirulina bioluminescent substances into the incubation mother solution of MOFs, and is prepared by a common solution crystallization method. The prepared bioluminescent MOFs have a definite crystal structure, and the maximum chromogenic wavelength is 620nm, showing strong blue fluorescence or red fluorescence. Background technique [0002] Fluorescent imaging, detection, and treatment technologies based on sensors have promoted the rapid development of biomedicine. People hope to observe the growth of tumor cells in living animals and their responses to drugs with the help of technologies such as fluorescently labeled peptides, small molecules, and antibodies. The development of metal-organic frameworks (MOFs) (Metal-Organic Frameworks) that can be used for biomedical imaging is of great significance for expandi...

AI Technical Summary

Problems solved by technology

However, the further functionalization of γ-CD-MOFs, such as improving biocompatibility and endowing bioluminescent properties, has not been reported.

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