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Novel endophytic fungus LZ3 and application thereof

A technology of new species and strains, applied in application, fungi, biocides, etc., can solve the problems of chemical fertilizers, pesticides, disease resistance, high yield and quality are difficult to balance and break through, and threaten the safety of environmental agricultural products.

Active Publication Date: 2020-02-18
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent decades, the use of chemical fertilizers and pesticides has greatly promoted the increase and stability of grain production. However, due to the difficulty in balancing and breaking through disease resistance, high yield and quality in breeding, and the long-term unreasonable use of chemical fertilizers and pesticides, Serious threat to the environment and the safety of agricultural products

Method used

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  • Novel endophytic fungus LZ3 and application thereof
  • Novel endophytic fungus LZ3 and application thereof
  • Novel endophytic fungus LZ3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The new strain Acidomelania saccharicola LZ3 in this example has a deposit number of CGMCC NO.18805. Belongs to the kingdom Fungi, the genus Acidomelania. Preserved in the General Microorganism Center of China Microbial Culture Collection Management Committee, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18805, and the preservation date is October 25, 2019.

[0029] The above new strains were collected from the sugarcane rhizosphere soil (N24°32′12.92″, E109°20′43.32″, 99.2 meters above sea level) in the sugarcane field of Shibeiping Village, Shibeiping Town, Liubei District, Liuzhou City, Guangxi, and isolated by trapping .

[0030] The above-mentioned isolated crop growth-promoting endophytic fungi are detected as follows:

[0031] 1. Morphological characteristics of the strain

[0032] The colony diameter of this strain is about 28mm after being cultured in oat (OA) at 28°C for two weeks, the colony is ...

Embodiment 2

[0037] Control effect on banana wilt:

[0038] Test plant: Guijiao No. 1 tissue culture seedling (provided by Guangxi Plant Tissue Culture Seedling Co., Ltd.); test strain: strain LZ3, obtained from sugarcane rhizosphere soil in Liuzhou, Guangxi by trapping and isolation in 2016; pathogen of banana wilt: Fusarium Physiological race 4 (FOC4) highly pathogenic strains.

[0039] 1. The control effect of plate:

[0040] (1) Co-cultivation of LZ3 and banana tissue culture seedlings:

[0041] The bacterial strain LZ3 was activated and inoculated on the oat medium (oat flour 10g / L, agar 18g / L, MgSO 4 ·7H 2 O1g / L, KH 2 PO 4 1.5g / L, NaNO 3 1g / L), each plate was connected with 3 bacterial blocks, and after 10 days of cultivation, the banana tissue culture seedlings with the same growth were selected and transplanted to the colony, the root system was spread on the surface of the colony, and each colony was transplanted with one tissue culture seedling. Transplanted 3, and named...

Embodiment 3

[0097] Growth-promoting effect on sugarcane:

[0098] Test plants: sugarcane Guitang 49 variety tissue culture seedlings (provided by Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences); test strains: strain LZ3, obtained from sugarcane rhizosphere soil in Liuzhou, Guangxi by trapping in 2016.

[0099] The specific method includes the following steps:

[0100] (1) Inoculate the activated LZ3 in PDB medium, shake and culture in a shaker at 28°C with a rotation speed of 120rpm for 10-17d, filter and collect the mycelial mass with sterile gauze, rinse with sterile water several times, Break up the mycelium mass to prepare a concentration of 5×10 5 cfu / ml bacterial solution;

[0101] (2) After the sugarcane tissue culture seedlings are grown to 5-7 leaves in the sandy land, select sugarcane seedlings with consistent growth, and trim the leaves to be planted; the sugarcane seedlings are transplanted to a size of 12cm * 12cm (height * diameter) In the seedli...

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Abstract

The invention relates to the technical field of biologics and in particular relates to a novel strain Acidomelania saccharicola LZ3 and application thereof. The novel strain LZ3 is separated from rhizosphere soil of sugarcane, and the preservation number is CGMCC NO.18805; according to taxonomy methods, the strain is a novel variety of the Acidomelania family, the taxonomic status of the strain isAcidomelania sp., according to naming methods of the International Bacterium System Taxonomy Committee, the strain is named as Acidomelania saccharicola sp.nov., and the strain LZ3 is selected as a type strain of the variety. Discovery and utilization of the novel strain enrich available microbial resources of endophytic fungi, and the strain has functions of preventing and treating banana wilt and promoting crop growth, and is capable of providing excellent culture resources in fields of plant growth promotion and agricultural application for disease and insect prevention.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a new strain LZ3 and its application. 【Background technique】 [0002] In recent decades, the use of chemical fertilizers and pesticides has greatly promoted the increase and stability of grain production. However, due to the difficulty in balancing and breaking through disease resistance, high yield and quality in breeding, and the long-term unreasonable use of chemical fertilizers and pesticides, Serious threat to the environment and the safety of agricultural products. Therefore, it is an urgent need to find new agricultural methods and new technologies that are cost-effective and environmentally compatible to solve the current environmental pollution and food safety crisis. It is of great ecological and economic significance to make full use of the microorganisms in the ecosystem to promote the growth of crops and resist diseases and insect pests to improve the production capac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00A01P21/00C12R1/645
CPCC12N1/145C12R2001/645
Inventor 龙艳艳农倩谢玲覃丽萍张艳陈艳露廖仕同张雯龙
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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